Bioavailability of Carotenoids Added Into Processed Foods
キーワード
概要
説明
Carotenoids are a diverse group of natural-occurring fat-soluble pigments. Whilst over 600 different types have been characterised around 20 have been found in human tissue in the postprandial state, however, over 90% of these absorbed carotenoids are made up of 6 specific types (alpha-Carotene, beta-carotene, Lycopene, Lutein, Zeaxanthin and Cryptoxanthin)
There is good evidence to suggest that carotenoids play a notable role in human health both through their pro-vitamin activity and as biological antioxidants. Carotenoids are believed to be responsible for the beneficial effects of fruits and vegetables in preventing human chronic diseases such as cancer and cardiovascular disease. Increasing fruit and vegetables intakes in individuals can sometimes be difficult and unsustainable, therefore, increasing bioavailability may be an effective alternative approach to maximise health outcomes.
A notable number of vegetables are rich sources of carotenoids, however, the amounts available to the human body for metabolism are often small due to poor bioavailability. For instance, studies have shown that bioavailability of carotenoids from carrots and tomatoes can be as low as 1-3%. The bioavailability of carotenoids has been shown to depend on factors such as their source, degree of cooking, processing, mechanical breakdown, presence of fat and interactions between carotenoids. Whilst the presence of fat significantly increases carotenoid absorption, the oil type, quantity and form it is in all affect bioavailability.
Recent work suggests that carotenoid bioavailability improves when fat is present in the form of mayonnaise rather than as plain oil. This may be due to the emulsion properties of mayonnaise which allows better solubilisation of amphiphilic compounds such as carotenoids. Mayonnaise has also been shown to be more miscible in gastric juices (compared to plain oil) and this too may be contributing to the greater absorption of carotenoids.
The present study builds upon this previous work and studies carotenoid bioavailability in processed food systems. Using a bread-mayonnaise meal the study compares carotenoid bioavailability when it is incorporated either into the bread or the mayonnaise. Furthermore, the study investigates the impact of mayonnaise on the bioavailability of carotenoids incorporated into a processed food (bread). The study will focus on the bioavailability of three principle carotenoids found in foods, alpha-carotene, beta-carotene and lycopene. Carrot and tomato are used as the sources of carotenoids.
HYPOTHESIS Carotenoid bioavailability is influenced by the food matrix in which they are contained.
OBJECTIVES
1. To compare the acute-phase bioavailability (systemic and gut absorption) of targeted carotenoids (alpha-carotene, beta-carotene and lycopene) when they are incorporated into the different processed food matrices (bread and mayonnaise)
2. Study how mayonnaise affects the acute bioavailability of carotenoids incorporated into a processed food.
日付
最終確認済み: | 02/29/2016 |
最初に提出された: | 09/17/2014 |
提出された推定登録数: | 09/17/2014 |
最初の投稿: | 09/22/2014 |
最終更新が送信されました: | 02/23/2017 |
最終更新日: | 02/26/2017 |
実際の研究開始日: | 06/30/2014 |
一次完了予定日: | 02/29/2016 |
研究完了予定日: | 06/30/2016 |
状態または病気
介入/治療
Dietary Supplement: Plain bread with plain mayonnaise
Dietary Supplement: Vegetable bread only
Dietary Supplement: Plain bread with vegetable mayonnaise
Dietary Supplement: Vegetable bread with plain mayonnaise
段階
アームグループ
腕 | 介入/治療 |
---|---|
Experimental: Plain bread with plain mayonnaise Control meal. The bread and mayonnaise in this meal does not have vegetable powders incorporated into them, hence this meal does not contain carotenoids | Dietary Supplement: Plain bread with plain mayonnaise The bread and mayonnaise in this meal does not have vegetable powders incorporated into them. Hence this meal does not contain carotenoids. |
Experimental: Vegetable bread only A vegetable powder (carrot and tomato) containing bread portion will be served alone. The amount of vegetable powder in the bread will be standardised to contain a known amount of carotenoids | Dietary Supplement: Vegetable bread only A vegetable powder (carrot and tomato) containing bread portion will be served alone. The amount of vegetable powder in the bread portion will be standardised to contain a known amount of carotenoids. |
Experimental: Vegetable bread with plain mayonnaise A vegetable powder (carrot and tomato) containing bread portion will be served with plain mayonnaise. The amount of vegetable powder in the bread will be standardised to contain a known amount of carotenoids. | Dietary Supplement: Vegetable bread with plain mayonnaise A vegetable powder (Carrot and tomato) containing bread portion will be served with plain mayonnaise. The amount of vegetable powder in the bread portion will be standardised to contain a known amount of carotenoids |
Experimental: Plain bread with vegetable mayonnaise Plain bread will be served with a vegetable powder (carrot and tomato) containing mayonnaise. The amount of vegetable powder in the mayonnaise will be standardised to contain a known amount of carotenoids. | Dietary Supplement: Plain bread with vegetable mayonnaise Plain bread will be served with a vegetable powder (carrot and tomato) containing mayonnaise. The amount of vegetable powder in the mayonnaise will be standardised to contain a known amount of carotenoids. |
適格基準
研究の対象となる年齢 | 18 Years に 18 Years |
研究に適格な性別 | All |
健康なボランティアを受け入れる | はい |
基準 | Inclusion Criteria: - Healthy males and females Aged between 18 and 75 years. Body mass index between 18.5 - 40 kg/m2. Blood pressure equal to or less than 139/89 mmHG. HbA1C less than or equal to 6.5%. Total blood cholesterol less than 6.0mmol/l Exclusion Criteria: - Suffering from Diabetes, kidney disease, hepatic disease, gout, gastrointestinal disorder, thromboembolic or coagulation disease, hypertension, thyroid disorder or hypercholesterolaemia On prescription medications Orlistat, Digoxin, Anti-arrhythmics, tricyclic anti-depressants, neuroleptics, oral anti-diabetic medication, insulin, anti-inflammatories, anti-pyretics and Statins Allergic/Intolerant to any of the foods in the study Vegetarian or Vegan Restricted eating and/or eating disorders Alcohol and/or substance abuse Regularly take nutritional supplements (once a day) Smoking Poor venous access and veins difficult to cannulate |
結果
主な結果の測定
1. Change in Carotenoid concentrations in plasma and in chylomicron-rich fraction [At baseline, at every hour for up to 8 hours postprandial and at 24hours after test meal]
二次的な結果の測定
1. Change in carotenoid concentration in urine [at baseline and at every hour for up to 8 hours post-prandial and at 24hours after test meal]
その他の成果対策
1. Measurement of plasma retinol and retinyl ester concentrations [at baseline and at every hour for up to 8 hours post-prandial and at 24 hours after the test meal]
2. Plasma cholesterol concentrations [At baseline and at every hour for up to 8 hours post-prandial and at 24hours after the test meal]
3. Satiety and palatability of test meals [immediately before and after consumption of test meals]