Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 2004-Dec
[Determination of sennoside A in rhubarb extracts by HPLC].
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概要
OBJECTIVE
To determine sennoside A in rhubarb extracts by solvent extraction and SFE-CO2.
METHODS
Sennoside A was extracted by ultrasonic vibration. The analytical column was Allitima RP C18 (250 x 4.6 mm, 5 microm). The mobile phase consisted of 0.1% TFA-HCN (44:56). The flow rate was 1.0 ml/min. The UV detector wavelength was 280 nm and the reference wavelength was 600 nm. The column temperature was 25 degrees C.
RESULTS
Considering the extracting rate of sennoside A, solvent extraction was better than SFE-CO2.
