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The Scientific World Journal 2012

Ethylene inhibitors enhance shoot organogenesis of gloxinia (Sinningia speciosa).

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Soo Cheon Chae
Haeng Hoon Kim
Sang Un Park

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概要

Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl₂), and silver thiosulphate (STS). The addition of AVG, CoCl₂, and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. The highest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performing highest regeneration frequency with highest number of shoots. This treatment (STS at 5 mg/L) produced 40% more shoots per explant compared to control followed by STS at 10 mg/L with increasing 37% more shoots compared to control. In the cases of AVG and CoCl₂ the highest shoot number per explant was found at 1 mg/L. Treated with AVG and CoCl₂ at 1 mg/L increased shoot number by 16 and 12%, respectively, compared to control. Ethylene inhibitors could be used as a possible micropropagation and plant transformation protocol in S. speciosa for plant regenerations.

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