Japanese
Albanian
Arabic
Armenian
Azerbaijani
Belarusian
Bengali
Bosnian
Catalan
Czech
Danish
Deutsch
Dutch
English
Estonian
Finnish
Français
Greek
Haitian Creole
Hebrew
Hindi
Hungarian
Icelandic
Indonesian
Irish
Italian
Japanese
Korean
Latvian
Lithuanian
Macedonian
Mongolian
Norwegian
Persian
Polish
Portuguese
Romanian
Russian
Serbian
Slovak
Slovenian
Spanish
Swahili
Swedish
Turkish
Ukrainian
Vietnamese
Български
中文(简体)
中文(繁體)
Chinese Journal of Chromatography (Se Pu) 2002-Mar

[Modification of the analytical method for rotenoids in plants].

登録ユーザーのみが記事を翻訳できます
ログインサインアップ
リンクがクリップボードに保存されます
Xin-Nian Zeng
Josep Coll
Shan-Xue Zhang
Xin-Qing Liu
Francisco Camps

キーワード

概要

Rotenoids are the active ingredients of some botanical insecticides and prospective candidates as anticancer agents. The proper isolation and determination of rotenoids in plants is of great importance for their further research and development. However, the HPLC method available for this purpose was developed particularly for the detection and determination of rotenone, so it appears to be unsuitable for the analysis of other rotenoids such as deguelin, elliptone and their analogues. By checking the UV spectra, it has been found that four types of UV absorption patterns occurred among the major rotenoids isolated from the roots of Derris elliptica and leaves of Tephrosia vogelii, and that the detection wavelength at 240 nm is more adequate for the analysis of a complex of rotenoids than at 280 nm-300 nm, which is used for rotenone detection. The extraction of rotenoids from plants is conveniently carried out by CHCl3-MeOH(9:1, V/V) and the purification can be accomplished by filtration of the crude residue through a C18 reversed-phase cartridge. Rotenoids can be isocratically eluted by MeOH-H2O(66:34, V/V). The results showed that rotenone, deguelin, elliptone, and their 12a-hydroxy- and 6a,12a-dehydro-analogs can be easily detected by the modified method, along with a satisfactory peak separation. The rotenoid components might be characterized by their retention times and relative retention times based on rotenone, which were at a range of 3.26 min-39.42 min and 40.4%-489.1% respectively.

Facebookページに参加する

科学に裏打ちされた最も完全な薬草データベース

  • 55の言語で動作します
  • 科学に裏打ちされたハーブ療法
  • 画像によるハーブの認識
  • インタラクティブGPSマップ-場所にハーブをタグ付け(近日公開)
  • 検索に関連する科学出版物を読む
  • それらの効果によって薬草を検索する
  • あなたの興味を整理し、ニュース研究、臨床試験、特許について最新情報を入手してください

症状や病気を入力し、役立つ可能性のあるハーブについて読み、ハーブを入力して、それが使用されている病気や症状を確認します。
*すべての情報は公開された科学的研究に基づいています

Google Play badgeApp Store badge