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Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences 2014-Mar

[Purification of polyphenols from Sabina vulgaris antoine and its antioxidant properties].

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Long Li
Gui-da Xuan
Ping Chen

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概要

OBJECTIVE

To purify polyphenols from Sabina vulgaris and to investigate its antioxidant properties.

METHODS

Polyphenols were purified from Sabina vulgaris Antoine with macroporous resin HPD-700, and the quantity of polyphenols was determined by Folin-Ciocalteu colorimetry. The antioxidant properties of polyphenols were evaluated by total antioxidant capacity (T-AOC) and its activities of scavenging DPPH (1,1 diphenyl-2-picry-hydrazyl) radicals, superoxide anion (O2·-), hydroxyl free radicals (OH·) and ferric reducing antioxidant power (FRAP).

RESULTS

After purification, the purity of polyphenols increased from 0.053% to 0.995%.The antioxidant properties study showed that its inhibition rate of scavenging DPPH radicals and FRAP was 151.83 U/ml and 204.59 U/ml. Its scavenging capacity for superoxide anion (O2·-) and hydroxyl free radicals (OH·) was 151.83 U/ml and 204.59 U/ml. The total antioxidant capacity was 72.68 U/ml.

CONCLUSIONS

Polyphenols from Sabina vulgaris Antoine have high antioxidant properties, suggesting that it worth further study of its pharmacological effects.

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