Kahweol Reduces Food Intake of Caenorhabditis elegans

The coffee diterpene kahweol may contribute to the anti-obesity effects of coffee, but its physiological effects have yet to be elucidated. Caenorhabditis elegans is used as an animal model in obesity research because its lipid metabolism is conserved in humans. The goal was to investigate kahweol's effects on lipid metabolism in C. elegans. Kahweol at 120 µM reduced fat accumulation by 17% compared to the control, which was associated with a reduced food intake. Kahweol did not reduce fat in eat-2 mutants, which have a disrupted pharynx contraction rate, suggesting that the fat-lowering effects of kahweol were dependent on food intake. Lipid metabolism-related gene homologs of tubby protein (tub-1), enoyl-CoA hydratase (ech-1.1), adipose triglyceride lipase (atgl-1), insulin/insulin-like growth receptor (daf-2) and forkhead box O transcription factor (daf-16) were also associated with changes in food intake by kahweol. Therefore, kahweol's fat-lowering effects are due to a reduction of food intake in C. elegans.