Seven isoquinoline alkaloids isolated from the bark of Actinodaphne macrophylla in this study demonstrated in vitro antiplasmodial activities against Plasmodium falciparum 3D7 with IC50 values of 0.08 µM, 0.05 µM, 1.18 µM, 3.11 µM, 0.65 µM, 0.26 µM, and 1.38 µM for cycleanine, 10-demethylxylopinine,