The subject collects the unstimulated saliva in a sterile glass dish for the measurement of baseline pH of the saliva.The subject is then given 100 ml of test carbonated drink, Coco cola ® to drink. The subject will sip, swish and swallow the drink within 2 minutes.
Unstimulated saliva samples are collected from the subject to measure the pH of saliva after 5, 15, 30, 45 and 60 minutes of consumption of the test carbonated drink.

The subject collects the unstimulated saliva in a sterile glass dish for the measurement of baseline pH of the saliva.The subject is then given 100 ml of test carbonated drink, Coco cola ® to drink. The subject will sip, swish and swallow the drink within 2 minutes.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva after 5 and 15 minutes of consumption of the test carbonated drink.
The subject will use 10 ml of tap water as mouth rinse to swish for 60 seconds and spit. Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes the gargle as an intervention

The subject collects the unstimulated saliva in a sterile glass dish for the measurement of baseline pH of the saliva.The subject is then given 100 ml of test carbonated drink, Coco cola ® to drink. The subject will sip, swish and swallow the drink within 2 minutes.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva after 5 and 15 minutes of consumption of the test carbonated drink.
The subject will use 10 ml of 0.2% Chlorhexidine mouth rinse (Rexidine®, Indoco Remidies Ltd, Mumbai, India) to swish for 60 seconds and spit. Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes the gargle as an intervention.

The subject collects the unstimulated saliva in a sterile glass dish for the measurement of baseline pH of the saliva.The subject is then given 100 ml of test carbonated drink, Coco cola ® to drink. The subject will sip, swish and swallow the drink within 2 minutes.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva after 5 and 15 minutes of consumption of the test carbonated drink.
The subject will Brush with fluoridated toothpaste-(Colgate Total®, Colgate-Palmolive Company, Mumbai, India) for 2 minutes using soft brush.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes the tooth paste as an intervention.

The subject collects the unstimulated saliva in a sterile glass dish for the measurement of baseline pH of the saliva.The subject is then given 100 ml of test carbonated drink, Coco cola ® to drink. The subject will sip, swish and swallow the drink within 2 minutes.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva after 5 and 15 minutes of consumption of the test carbonated drink.
The subject will chew polyol containing gum (Orbit®, WrigleyCompany) for 5 minutes and spit.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes the chewing gum as an intervention.

The subject collects the unstimulated saliva in a sterile glass dish for the measurement of baseline pH of the saliva.The subject is then given 100 ml of test carbonated drink, Coco cola ® to drink. The subject will sip, swish and swallow the drink within 2 minutes.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva after 5 and 15 minutes of consumption of the test carbonated drink.
The subject will use 10 ml freshly prepared 1% sodium bicarbonate w/v solution to swish for 60 seconds and spit.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes the gargle as an intervention

Saliva Altered

Oral Hygiene

Tooth Structure; Disorder

Unstimulated saliva samples are collected from the subject to measure the pH of saliva after 5, 15, 30, 45 and 60 minutes of consumption of the test carbonated drink without any intervention

Group 1: no intervention

The subject will use 10 ml of tap water as mouth rinse to swish for 60 seconds and spit. Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes the gargle as an intervention.

Group 2: tap water gargle

The subject will use 10 ml of 0.2% Chlorhexidine mouth rinse (Rexidine®, Indoco Remidies Ltd, Mumbai, India) to swish for 60 seconds and spit. Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes the gargle as an intervention

Group 3: 0.2% Chlorhexidine

The subject will Brush with fluoridated toothpaste-(Colgate Total®, Colgate-Palmolive Company, Mumbai, India) for 2 minutes using soft brush.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes using tooth paste as an intervention.

Group 4: Fluoridated tooth paste

The subject will chew polyol containing gum (Orbit®, WrigleyCompany) for 5 minutes and spit.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes chewing gum as an intervention.

Group 5: Polyol containing gum

The subject will use 10 ml freshly prepared 1% sodium bicarbonate w/v solution to swish for 60 seconds and spit.
Unstimulated saliva samples are collected from the subject to measure and record the pH of saliva at 15, 30 and 45 minutes after the subject completes the gargle as an intervention.

Group 6: 1% sodium bicarbonate solution

Department of Oral Medicine & Radiology, Uttaranchal Dental & Medical Research Institute

Comparison of 5 Buffering Agents on Changes in Salivary pH in Individuals Previously Exposed to a Test Carbonated Drink

Comparison of Five Different Salivary Buffering Agents on the Changes in Salivary pH in a Cohort Previously Exposed to a Test Carbonated Drink: A Randomized Controlled Crossover Trial.

All subjects in all groups collects the unstimulated saliva in a sterile glass dish for the measurement of baseline pH of the saliva. The subject will give the saliva sample just before being exposed to the test carbonated drink by swishing 100ml of CocaCola in mouth for 2minutes and then drinking it The outcome accessor is blinded to origin of sample (from which group).

All subjects will be given 100 ml of test carbonated drink, Coco Cola ® to drink. The subject will sip, swish and swallow the drink within 2 minutes. Five minutes after this, each subject will give unstimulated saliva in a sterile dish for measurement of saliva -5minutes after exposure to test carbonated drink. The outcome accessor is blinded to the origin of sample (from which group).

All subjects will give unstimulated saliva in a sterile dish for measurement of saliva -15minutes after exposure to test carbonated drink. The outcome accessor is blinded to the origin of sample (from which group).

All subjects from all six (5test and 1comparator) groups will perform the assigned intervention as per schedule at time 15minutes post exposure to carbonated drink and shall give unstimulated saliva in a sterile dish for measurement of saliva -30minutes after exposure to test carbonated drink or in other words 15minutes after assigned intervention. The outcome accessor is blinded to the origin of sample (from which group).

All subjects from all six (5test and 1comparator) groups will perform the assigned intervention as per given schedule at time 15minutes post exposure to carbonated drink and shall give unstimulated saliva in a sterile dish for measurement of saliva -45minutes after exposure to test carbonated drink or in other words 30minutes after assigned intervention. The outcome accessor is blinded to the origin of sample (from which group).

All subjects from all six (5test and 1comparator) groups will perform the assigned intervention as per given schedule at time 15minutes post exposure to carbonated drink and shall give unstimulated saliva in a sterile dish for measurement of saliva -60minutes after exposure to test carbonated drink or in other words 45minutes after assigned intervention. The outcome accessor is blinded to the origin of sample (from which group).

Dr Meru S

Prof & Head, Dept of Oral Medicine & Radiology

only primary investigator will know the intervention done by the subject. The outcome investigator(assessor) will be unaware (blinded) to which intervention the subject is undertaken.

Those who smoke at least one cigarette a day.

Those who have not smoked for at least one week when the sample is collected.

Smoking

Avioq

Evaluation of An Oral Fluid Collection Device

Collection and Testing of Human Oral Fluid Samples/VioOne OF Sample Collection Device

ELISA assay in comparison with the Mass-spec method will be used to detect the presence of nicotine in the oral fluid samples collected with the VioOne collection device.

cola/dental caries

Comparison of 5 Buffering Agents on Changes in Salivary pH in Individuals Previously Exposed to a Test Carbonated Drink

Evaluation of An Oral Fluid Collection Device

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