ページ 1 から 61 結果
In this study, epimedins A-, B-, and C-, and icariin-rich extracts were simultaneously isolated from Epimedium brevicornu Maxim. through a convenient four-stage process consisting of solvent extraction, macroporous resin column pre-concentration, extraction fractionation, and reversed-phase (RP)
The concentration variations of main flavonoids, epimedins A-C and icariin, among ten representative populations of Epimedium brevicornu Maxim. were assessed by HPLC. The populations were collected during the flowering stage and included 419 individual samples. Remarkable variations within and among
Reports elsewhere demonstrated that Epimedin C, a constituent isolated from the leaves of Epimedium sagittatum, possessed anti-tumor activity. However, its mechanism of action remains unresolved. Using SK-Hep-1 cells, a poorly-differentiated hepatoma subline, as an experimental model, we present
Epimedium brevicornum Maxim is a famous medicinal herb which has been widely used for the treatment of impotence, infertility, osteoporosis, cardiovascular diseases, amnesia, and senile functional diseases. A dual-mode high-speed counter-current chromatographic method is developed for the separation
The genus Epimedium is rich in terms of flavonoids, of which icariin, epimedin A, epimedin B and epimedin C are known especially to be biologically active. Therefore, it is important to quantify these compounds. In this study, a HPLC method coupled with DAD detection was developed and validated for
Callus cultures of Epimedium diphyllum produced a large amount of epimedoside A in addition to a small amount of diphylloside B, ikarisoside C, epimedoside E, diglycosides of des-O-methylanhydroicaritin (8-gamma, gamma-dimethylallylkaempfero). Icariin, epimedins A-C, which are glycosides of
Six chemical constituents were isolated from the whole plant of Epimedium fargesii and identified as epimedoside A, ikarisosids C, epimedin B, epimedin C, icariin and hyperin by means of UV, IR, 1H-NMR, 13C-NMR and MS spectral analysis. They were isolated from this plant for the first time.
This study characterizes the correlation between the chemical fingerprint and estrogenic activity of an Epimedium koreanum extract. The estrogenic activity of 31 E. koreanum extract samples was evaluated by a luciferase reporter gene assay, and the samples were classified into 3 groups based on
Seven flavonoids named diphylloside A, epimedoside A, epimedin C, icariin, epimedoside C, icarisoside A, desmethylanhydroicaritin, as well as the oleanolic acid, were isolated from the roots of Epimedium wushanense for the first time. These flavonoids manifested significant antioxidant activity in
A new HPLC-DAD-ESI-MS(n) method was developed for rapid separation, characterization and quantitation of flavonoids in Epimedium wushanense, a popular Chinese herbal medicine. For qualitative identification, a total of 37 compounds were characterized from the underground and aerial parts of E.
OBJECTIVE
To investigate the chemical constituents of Epimedium brevicornum.
METHODS
The chemical constituents were isolated by using silica gel column chromatography and preparative TLC. The structures were identified on the basis of physical-chemical constants and spectral data.
RESULTS
Five
Icariin and epimedins A, B, C are a series of active flavonol glycoside in Epimedium brevicornum Maxim. A pilot-scale preparative high performance liquid chromatographic (HPLC) method was developed to purify the four flavonol glycosides as reference standards from the crude extract of Epimedium
A micellar electrokinetic capillary chromatographic (MEKC) method with diode-array detection is developed for the characterization of pharmacologically active flavonoids in extracts prepared from Epimedium brevicornum, E. humanense, E. coactum, and E. truncatum. The pK(a) values of icarin, epimedin
The Epimedium herb, Yinyanghuo in Chinese, is a famous Chinese herbal medicine. In this study, an efficient extraction method was developed for the extraction of major bioactive constituent epimedin A, epimedin B, epimedin C and icariin from E. pubescens Maxim. using deep eutectic Epimedium herb (Yinyanghuo), one of the popular Chinese materia medica, is a multiple species colony of Epimedium genus belonging to Berberidaceae. There are five species of Epimedium that have been officially adopted in Chinese Pharmacopoeia under the same crude drug name 'Yinyanghuo' comprising