thebaine/papaver bracteatum

Root cultures derived from cell suspension cultures of Papaver bracteatum were shown to produce thebaine (yield 0.03%).

A rapid colorimetric test for the determination of thebaine in dried, extracted Papaver bracteatum is described. The method is based on the development of an orange color formed by the nitroso color reaction for phenols. The absorption peak is near 450 nm. The linear range response is from 0 to 1.0

An interlaboratory procedure for the quantitative extraction and analysis of thebaine from different tissues of Papaver bracteatum Lindl. is presented. Each step was evalutated for the yield of thebaine by use of 1-3H-thebaine and GLC. The method of drying and milling of tissue and the size of

Papaver bracteatum is an important source of opiates because of its high thebaine content. An efficient and convenient method of determining thebaine, not only in the root, but in the more highly pigmented capsule, stem and leaf, is urgently required. We decided to examine these in detail on a wide

A method is described for the quantitative analysis of thebaine from Papaver bracteatum, using a single high-pressure liquid chromatographic column. The procedure gives base-line separation of thebaine without the need for gradient elution equipment, and can be completed within 12 min. Thebaine

The fungicides benlate, maneb, and captan depressed plant growth, thebaine concentration, and thebaine yield per plant when administered chronically to Papaver bracteatum. The chronic administration of dicofol inhibited plant growth and thebaine yield per plant, but did not affect thebaine

Plants have been regenerated from embryogenic callus cultures of two varieties of Papaver bracteatum and successfully transplanted to soil. Regeneration occurred in good yield, around 40 plants to soil within 5 months per 0.4g piece of original callus. Thebaine concentrations comparable to those in

The heritability and repeatability of various characters affecting thebaine and seed yields in Papaver bracteatum were estimated from parents and progeny families during two consecutive years of growth. Thebaine and seed yields per plant showed the lowest heritability and therefore low selection

The alkaloids, orientalidine, oripavine, thebaine, narceine, narceine, codeine, papaverine, isothebaine, alpenigenine and morphine react with a solution of sulfuric acid containing molybdate to give specific colors. Using the acid solution permitted the differentiation of Papaver bracteatum latex

A simple spectrophotometric determination of thebaine based on the complexation reaction with bromcresol green was developed. The yellow complex was extracted with chloroform over the pH 1.5--4.5 range. The solution of the complex in chloroform showed the maximum absorption at 415 nm and obeyed

The thebaine-yield components in the roots of PAPAVER BRACTEATUM were studied at various stages of plant development. The maximum concentration of thebaine was obtained at the start of flowering. However the dry weight of the roots and the thebaine yield increased until full flowering. Significant

Several solvents have been examined in the process of developing a convenient method for extracting powdered material and removing interfering pigments to produce an extract of Papaver bracteatum suitable for g.l.c. assay. The most suitable extracting solvent was aqueous acetic acid (5%); the assay

Plants of Papaver bracteatum Lindl. derived from different germplasm sources within one Iranian collection were grown for 18 and 37 weeks in the greenhouse and controlled environment chambers. Gas-chromatographic analyses showed significant differences between thebaine concentrations of root tissue

From colchicine-treated seeds of Papaver bracteatum Lindl. some poppy plants were obtained that developed capsules richer in thebaine than the controls. The individual poppies were analyzed for capsule thebaine content annually for eight successive years, the results revealing significant

The biosynthesis path engineering could be very promising for mass production of alkaloids by applying elicitors in the cell suspension culture of Persian poppy (Papaver bracteatum Lindl.). In this work, the effects of different concentrations of methyl jasmonate (MJ) and phloroglucinol (PG) on