Biomarkers of Exposure to Air Pollutants (AEROTOX-2)
Raktažodžiai
Santrauka
apibūdinimas
To estimate the influence of the atmospheric pollution on the whole adult population, subjects will be recruited in a wide range of age (20 - 80 years).
Samples of atmospheric particles will be collected in Dunkerque (North of France), one of the most important industrial areas in France. This city is influenced by car traffic, urban and industrial activities, and the wide sea traffic of the North Sea. Therefore, Dunkerque is a reference site to study the atmospheric pollution and its impact on health.
These markers will be searched in association with the different mechanisms involved in the toxicity of the fine particles such as: inflammatory response, genotoxicity, metabolic activity and epigenetic changes after exposure of leukocytes isolated from patients to doses of fine particles.
The study of the correlation between biological observations and conditions of exposure to air particles will bring answers about the impact of the environmental pollution on the health of vulnerable populations.
Datos
| Paskutinį kartą patikrinta: | 12/31/2016 |
| Pirmasis pateikimas: | 04/24/2016 |
| Numatytas registravimas pateiktas: | 05/04/2016 |
| Pirmas paskelbtas: | 05/05/2016 |
| Paskutinis atnaujinimas pateiktas: | 01/05/2017 |
| Paskutinis atnaujinimas paskelbtas: | 01/08/2017 |
| Faktinė studijų pradžios data: | 10/31/2015 |
| Numatoma pirminio užbaigimo data: | 11/30/2016 |
| Numatoma studijų užbaigimo data: | 11/30/2016 |
Būklė ar liga
Intervencija / gydymas
Procedure: Blood sampling
Fazė
Rankų grupės
| Ranka | Intervencija / gydymas |
|---|---|
| Experimental: 20-30 years Blood sampling | |
| Experimental: 45-55 years Blood sampling | |
| Experimental: 70-80 years Blood sampling | |
| Experimental: Control Blood sampling for standardization of technical procedures |
Tinkamumo kriterijai
| Amžius, tinkami studijuoti | 20 Years Į 20 Years |
| Tinkamos studijoms lytys | All |
| Priima sveikus savanorius | Taip |
| Kriterijai | Inclusion Criteria: - Age between 20-30 years, 45-55 years or 70-80 years - Signature of an informed consent - Non-smoker or ex-smoker for more than 10 years - Homogeneous repartition between men and women - Social insurance affiliation - Understanding or being able to speak French Exclusion Criteria: - Pregnant or breast feeding woman - Treatment with parenteral corticoids in the 30 days prior to inclusion visit, treatment with immunosuppressant drugs, radiotherapy, chemotherapy - Occupational exposure (metallurgy, petrochemistry, house painter) for more than 10 years and having ceased all activities in these areas for less than 10 years ago. |
Rezultatas
Pirminės rezultatų priemonės
1. Level of expression of blood biomarkers of air pollution exposure [through the study completion, an average of 48 months]
Antrinės rezultatų priemonės
1. Total amount of different populations of peripheral blood leucocytes [through the study completion, an average of 48 months]
2. Measure of the expression of genes involved in metabolism by quantitative polymerase chain reaction (qPCR) [through the study completion, an average of 48 months]
3. Measure of the expression of genes involved in metabolism by TaqMan Gene Expression Assays [through the study completion, an average of 48 months]
4. Detection of genotoxicity by identification of DNA double-strand breaks and DNA adducts [through the study completion, an average of 48 months]
5. Detection of epigenetic modifications [through the study completion, an average of 48 months]
6. Correlation between age and markers of inflammation [through the study completion, an average of 48 months]
7. Correlation between age and markers of metabolic activity [through the study completion, an average of 48 months]
8. Correlation between age and markers genotoxicity [through the study completion, an average of 48 months]
9. Correlation between age and markers of epigenetic modifications [through the study completion, an average of 48 months]
