Kinetics of Metabolic Cofactors in NAFLD
Raktažodžiai
Santrauka
apibūdinimas
In 10 healthy subjects with BMI <30 kg/m2 the plasma concentrations of 4 natural compounds (nicotinamide riboside, L-carnitine, L-serine and N-acetylcystein) are measured by ultra-performance liquid chromatography-tandem mass spectrometry (UPLCMSMS) after individual and combined administration, on five consecutive days and at every hour during 9 hours after administration. The study will start at 8:00 every morning and at each time point, blood samples will be collected.
The taste and any potential sensing of the co-factors such as vertigo, nausea, bowel movement will be recorded.
Each participant will receive one oral dose of Day 1: 1 g nicotinamide riboside; Day 2: 3 g L-carnitine; Day 3: 5 g N-acetylcystein; Day 4: 20 g L-serine; Day 5: combined 1 g nicotinamide riboside, 3 g L-carnitine, 5 g N-acetylcystein, and 20 g L-serine
In addition, untargeted metabolomics analysis as well as O-link proteomics analysis we be performed to study effect of the administered cofactors.
Datos
| Paskutinį kartą patikrinta: | 01/31/2019 |
| Pirmasis pateikimas: | 02/03/2019 |
| Numatytas registravimas pateiktas: | 02/10/2019 |
| Pirmas paskelbtas: | 02/11/2019 |
| Paskutinis atnaujinimas pateiktas: | 02/11/2019 |
| Paskutinis atnaujinimas paskelbtas: | 02/14/2019 |
| Faktinė studijų pradžios data: | 08/31/2018 |
| Numatoma pirminio užbaigimo data: | 10/31/2018 |
| Numatoma studijų užbaigimo data: | 11/30/2018 |
Būklė ar liga
Intervencija / gydymas
Dietary Supplement: Intervention
Fazė
Rankų grupės
| Ranka | Intervencija / gydymas |
|---|---|
| Experimental: Intervention Oral administration of cofactors, 1g nicotinamide riboside, 3g L-carnitine, 20g serine and 5g N-acetylcystein, first as single compounds, then combined, on 5 days | Dietary Supplement: Intervention Oral administration of 1g nicotinamide riboside, 3g L-carnitine, 20g serine and 5g N-acetylcystein, first as single compounds, then combined, on 5 days |
Tinkamumo kriterijai
| Amžius, tinkami studijuoti | 18 Years Į 18 Years |
| Tinkamos studijoms lytys | Male |
| Priima sveikus savanorius | Taip |
| Kriterijai | Inclusion Criteria: Healthy without any medication, no smokers, no obesity Exclusion Criteria: Any known disease, obesity |
Rezultatas
Pirminės rezultatų priemonės
1. Changes of plasma levels of nicotinamide riboside measured by mass spectrometry [Twenty-four hours after administration]
2. Changes of plasma levels of L-carnitine measured by mass spectrometry [Twenty-four hours after administration]
3. Changes of plasma levels of L-serine measured by mass spectrometry [Twenty-four hours after administration]
4. Changes of plasma levels of N-acetylcystein measured by mass spectrometry [Twenty-four hours after administration]
Antrinės rezultatų priemonės
1. Changes in the plasma level of metabolites associated with the supplementation of metabolic co-factors. [Twenty-four hours after administration of combined cofactors]
2. Changes in the plasma level of inflammation-related proteins associated with the supplementation of metabolic co-factors. [Twenty-four hours after administration of combined cofactors]
