Zhongguo Zhongyao Zazhi 2000-Dec
[Determination of nodakenin in Peucedanum].
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OBJECTIVE
To establish a HPLC method to determine the contents of nodakenin in Peucedanum.
METHODS
The determination method was achieved by RP-HPLC on an ODS column by UV detection at 334 nm; with nodakenin as external standard and H2O-CH3OH-CH3CN(75:5:20) as mobile phase.
RESULTS
Several samples of 6 species of Peucedanum were analyzed by this method. The contents of nodakenin ranged from 0.005%-3.090%.
CONCLUSIONS
Nodakenin cannot be used to control the quality of Peucedanum for pharmaceutical use, but this method is a simple and feasible way to control the quality of P. decursivum.
