A new cyclopeptide alkaloid from Clematis Florida

One new 14-membered ring cyclopeptide alkaloid, plenane A (1), along with six known compounds (2-7), was isolated from the roots of Clematis florida. Their structures were elucidated by means of NMR spectroscopic analysis and mass spectrometry. In addition, their anti- inflammatory effects on lipopolysaccharide-induced RAW264.7 macrophages were evaluated in vitro. The compounds 1, 2 and 6 exhibited potent indirect inhibitory effects, with IC₅₀ values of 40.92, 22.88 and 6.32 μM, respectively.

Keywords: Clematis florida; RAW264.7 macrophages; anti-inflammatory; cyclopeptide alkaloid.