Laboratory investigations :
complete blood count
renal and liver function tests
serum ferritin
lipid profile
Interleukin -6
HsC-RP
Adiponectin level
Imaging :
Abdominal ultrasound
Echocardiography
Carotid intima media thickness

Thalassemia

serum samples used for doing the test by ELISA

Interleukin-6

HsC-RP

Adiponectin level

Carotid intima media thickness

Assiut University Hospital

Adiponectin, IL-6 and hsC-RP in Relation to Carotid Intima-media Thickness in B-thalassemia Patients

Adiponectin, Interleukin-6 (IL-6) and High Sensitive C-reactive Protein (hsC-RP) in Relation to Carotid Intima-media Thickness in B-thalassemia Patients

Asmaa Nady Hussein

Principal investigator

Assiut University

Blood samples will be stored temporarily in the laboratory of the Principal Investigator at the University of Illinois at Chicago, long term storage of specimens will be at the UIC Biobank. The research data will be stored in a locked file cabinet in the PI's research office. The patient's name or other identifying data will not be revealed, except to those directly involved in this study.

Sickle Cell Disease

Diabetes Mellitus

University of Illinois at Chicago

Glucose Metabolism in Sickle Cell Disease

The investigator will use the ATP III guidelines for definition of metabolic syndrome. A combination of any three of the following criteria will lead to the designation of metabolic syndrome:
waist circumference >102 cm (men) or >88 cm (women)
triglycerides ≥150 mg/dL
HDL cholesterol <40 mg/dL (men) or <50 mg/dL (women)
blood pressure ≥130/≥85 mg/dL (or recorded diagnosis of hypertension and use of antihypertensives)
fasting glucose ≥110 mg/dL (or diagnosis of diabetes and use of anti-diabetic medications)

This will be accomplished by DNA linkage analysis and/or mutation analysis. In addition RNA will be isolated from from PBMCs and fractions of platelets, granulocytes and reticulocytes. The investigators will analyze the expression of transcripts to determine if alterations can explain the clinical observations.

Victor Gordeuk

Director Sickle Cell Center

A pilot study will be done on 20 patients with abnormal glucose tolerance which will include:
A-Continuous glucose monitoring system (CGMS) : A glucometer will be given to each patient and will be asked to measure blood glucose before meals and snacks and record the valus in the CGMS for better calibration (Khammar A et al., 2009).
B-Therapeutic intervention:
Thalassemia patients who proved to have diabetes according to the ADA criteria will be put on sc insulin

A pilot study will be done on 20 patients with abnormal glucose tolerance which will include:
A-Continuous glucose monitoring system (CGMS) : A glucometer will be given to each patient and will be asked to measure blood glucose before meals and snacks and record the valus in the CGMS for better calibration (Khammar A et
B-Therapeutic intervention:
Thalassemia patients who proved to have diabetes according to the ADA criteria will be put on insulin pump

Diabetes

Prediabetes

B-Thalassemia

A-Continuous glucose monitoring system (CGMS) : A glucometer will be given to each patient and will be asked to measure blood glucose before meals and snacks and record the valus in the CGMS for better calibration (Khammar A et al., 2009).
B-Therapeutic intervention:
Thalassemia patients who proved to have diabetes according to the ADA criteria will be subjected to
• Insulin pump will be tried in each diabetic thalassemic patient versus conventional insulin therapy(Bruttomesso D et al., 2009).

insulin pump/CGMS

The Use of CGMS to Detect Alterations of Blood Glucose in Thalassemic Patients

Evaluation of Continuous Blood Glucose Monitoring Method for Detection of Alterations in Glucose Homeostasis in Beta Thalassemia Patients

to find out the number of patients with thalassemia major having impaired glucose tolerance

to find out the number of patients with thalassemia major having diabetes mellitus

to compare the use of conventional insulin therapy to insulin pump in diabetic thalassemic patients

beta-thalassemia/nutukimas

Adiponectin, IL-6 and hsC-RP in Relation to Carotid Intima-media Thickness in B-thalassemia Patients

Glucose Metabolism in Sickle Cell Disease

The Use of CGMS to Detect Alterations of Blood Glucose in Thalassemic Patients

Išsamiausia vaistinių žolelių duomenų bazė, paremta mokslu