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smooth/skopolaminas
Nuoroda įrašoma į mainų sritį
Puslapis 1 nuo 130 rezultatus
Nicotinic acid action on gastrointestinal smooth muscle.
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The mechanism of nicotinic acid action in smooth muscle was studied by testing the drug in rat stomach fundus and descending colon using either the superfusion or perfusion technique. In the rat stomach strips nicotinic acid (or Na-nicotinate) induces concentration-dependent contractions that are
Characterization of muscarinic receptors in dog tracheal smooth muscle cells.
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1. The tritiated muscarinic antagonist N-methyl scopolamine, [3H]-NMS, was used to characterize the muscarinic receptors associated with the intact dog tracheal smooth muscle cells. Based on receptor binding assays, the intact tracheal smooth muscle cells had specific, saturable, high-affinity
Biochemical characteristics of muscarinic cholinoreceptors in swine tracheal smooth muscle.
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The tritiated muscarinic cholinoreceptor antagonist quinuclidinyl benzilate, [3H]QNB, was used to characterize the muscarinic receptors associated with homogenized membrane of the smooth muscle from swine trachea. Based on receptor binding assays, the homogenate had specific, saturable,
Characterization of muscarinic receptors on guinea pig gallbladder smooth muscle.
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BACKGROUND
Cholinergic agonists are of major importance for the regulation of gallbladder motility. However, the gallbladder muscarinic receptors have not been localized or characterized directly using radioligands, and it has not been clearly established which subtype of muscarinic receptor
Development of the muscarinic receptor in rabbit gastric smooth muscle.
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We used binding of [N-methyl-3H]scopolamine ( [3H]-NMS) to tissue homogenates and isometric contraction of muscle strips to characterize perinatal changes in the muscarinic receptor on rabbit gastric smooth muscle. In homogenates from fetal (28 days of gestation), 1-, 3-, and 7-day, 4- and 11-wk-old
Muscarinic receptors in isolated smooth muscle cells from gastric antrum.
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Smooth muscle cells from the gastric antrum of the rabbit were isolated using collagenase and pronase. We examined the characteristics of muscarinic receptors that control contraction of the muscle cell: kinetics, stoichiometry and specificity of both contractile response to muscarinic agents and
Characterization of muscarinic receptors in guinea pig ileum longitudinal smooth muscle.
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Heterogeneity in the muscarinic receptor population of guinea pig ileum longitudinal smooth muscle was found in competition binding experiments against N-methyl[3H]scopolamine using either a cardioselective (AF-DX 116) or a smooth muscle-selective (hexahydrosiladifenidol) antimuscarinic compound.
The role of calcium in amrinone action on intestinal smooth muscle.
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In the guinea-pig isolated ileum amrinone (3 microM to 0.3 M) induced transient, dose-dependent contractions which were followed by pronounced relaxation only at the highest amrinone concentrations tested (30 mM to 0.3 M). The contractile effect was potentiated by eserine and abolished by
Arteriolar smooth muscle responses are modulated by an intramural diffusion barrier.
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Arterioles (40-80 micron diameter) were isolated from the hamster cheek pouch, cannulated at both ends, and perfused with 3-(N-morpholino)propanesulfonic acid (MOPS)-buffered physiological salt solution (PSS). The vessels were observed with an inverted microscope and video system, and arteriolar
Effects of arachidonic acid hydroperoxides on vascular and non-vascular smooth muscle.
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The omega-6 and omega-9 hydroperoxides of arachidonic acid (AA) caused dose-dependent contraction of rabbit aortic strip (RAS) and guinea pig ileum (GPI) at concentrations between 5 and 200 microM. At these concentrations, arachidonic acid had no effect in these preparations. The contractions could
Adrenergic receptors on smooth muscle cells isolated from human penile corpus cavernosum.
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The effect of the alpha-adrenergic blocker moxisylyte was examined on smooth muscle cells isolated from human corpus cavernosum, and compared with that of other adrenergic agents and papaverine. Isolated smooth muscle cells were shown to contract (reduction of the mean cell length) under
Contraction and [3H]QNB binding in collagenase isolated fundic smooth muscle cells.
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Smooth muscle cells from the guinea pig gastric fundus were isolated by successive collagenase digestions. Tritiated quinuclidinyl benzilate [( 3H]QNB) was used to study the binding characteristics of the muscarinic cholinergic receptors on these cells. Each cell bound 8.3 X 10(-19) mol of QNB, and
Lidocaine attenuates muscarinic receptor-mediated inhibition of adenylyl cyclase in airway smooth muscle.
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We examined how lidocaine affects muscarinic receptor-mediated inhibition of adenylyl cyclase in bovine tracheal smooth muscles. Lidocaine (100 microM) augmented the relaxant responses to forskolin in the bovine tracheal smooth muscle contracted with methacholine (0.3 microM). On the other hand,
Antimuscarinic action of liriodenine, isolated from Fissistigma glaucescens, in canine tracheal smooth muscle.
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1. The antimuscarinic properties of liriodenine, isolated from Fissistigma glaucescens, were compared with methoctramine (cardioselective M2 antagonist) and 4-diphenylacetoxy-N-methylpiperidine (4-DAMP, smooth muscle selective M3 antagonist) by radioligand binding tests, functional tests and
PKC delta-isoform translocation and enhancement of tonic contractions of gastrointestinal smooth muscle.
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PKC is involved in mediating the tonic component of gastrointestinal smooth muscle contraction in response to stimulation by agonists for G protein-coupled receptors. Here, we present pharmacological and immunohistochemical evidence indicating that a member of the novel PKC isoforms, PKC-delta, is
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