najas flexilis/albumin

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Haemolysis of washed human red cells by the combined action of Naja naja phospholipase A 2 and albumin.

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A non-toxic anticoagulant metalloprotease: purification and characterization from Indian cobra (Naja naja naja) venom.

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Ielogoties Reģistrēties

A non-toxic potent anticoagulant metalloprotease NN-PF(3) has been purified to homogeneity from the Indian cobra (Naja naja naja) venom through a combination of column chromatography and electrophoresis. NN-PF(3) is a single chain protein with a molecular weight of 68 kDa by SDS-PAGE. It hydrolysed

Therapeutic potential of Naja naja atra venom in a rat model of diabetic nephropathy.

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OBJECTIVE To study the protective effects of naja naja atra venom (NNAV) in a rat model of diabetic nephropathy (DN). METHODS The rat diabetes model was induced by intraperitoneal injection of streptozotocin (STZ). Thirty-two model rats were randomly divided into one DN group (n=8) and three

Proteomic characterization of venom of the medically important Southeast Asian Naja sumatrana (Equatorial spitting cobra).

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Ielogoties Reģistrēties

The proteome of Naja sumatrana (Equatorial spitting cobra) venom was investigated by shotgun analysis and a combination of ion-exchange chromatography and reverse phase HPLC. Shotgun analysis revealed the presence of 39 proteins in the venom while the chromatographic approach identified 37 venom

[Comparative study of the immunogenicity of various forms of Naja oxiana cardiotoxin].

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Comparative study of the immune sera from rabbits immunized with both formol and native cardiotoxins, cardiotoxin incorporated into sphingomyelin-phosphatidylcholine-cholesterol liposome stabilized with osmium tetroxide, and a cardiotoxin-succinylated bovine albumin conjugate was performed. The

Cardiotoxic effects of Naja nigricollis venom phospholipase A2 are not due to phospholipid hydrolytic products.

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N. nigricollis phospholipase A2 (200 micrograms) has marked cardiotoxic actions on the perfused rat heart including the induction of arrhythmias, increases in ventricular thresholds, conduction and resting tension, and decreases in contractile tension. In contrast, perfusion with lysophosphatidyl

Cytotoxicity of cobra (Naja naja kaouthia) venom on rabbit red blood cells and S-180 tumor cells in the presence of tetracaine, lidocaine and procaine.

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Ielogoties Reģistrēties

The cytocidal action of Naja naja kaouthia venom on rabbit red blood cells and S-180 tumor cells treated with local anesthetics (tetracaine, lidocaine and procaine) were studied. The S-180 cells were more sensitive to the venom than the red blood cells which required albumin for efficient hemolysis

[Effect of phospholipase A2 from Naja naja oxiana venom on activity of Na+,K+-ATPase isoenzymes in rat brain].

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Ielogoties Reģistrēties

The specific sensitivity of the isoforms of the rat brain Na+,K(+)-ATPase catalytic subunit to phospholipase A2 (PL A2) from Naja naja oxiana venom has been estimated on the basis of changes in the two-component dose response curve of ouabain inhibition of Na+,K(+)-ATPase activity. Moderate

Purification and properties of a fibrinogenase from the venom of Naja nigricollis.

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Ielogoties Reģistrēties

A fibrinogenolytic proteinase from the venom of Naja nigricollis was purified by chromatography on Bio-Rex 70 and Phenyl-Sepharose. The purified enzyme, designated proteinase F1, was homogeneous by the criterion of SDS-polyacrylamide gel electrophoresis, and consisted of a single chain with a

Proteome and immunome of the venom of the Thai cobra, Naja kaouthia.

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Ielogoties Reģistrēties

The proteome of the Thai cobra, Naja kaouthia, venom, revealed by two-dimensional liquid chromatography/tandem mass spectrometry, was found to consist of peptides which could be matched with 61 proteins in the database. These proteins were classified into 12 groups according to the differences in

Ameliorating Adriamycin-Induced Chronic Kidney Disease in Rats by Orally Administrated Cardiotoxin from Naja naja atra Venom.

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Ielogoties Reģistrēties

Previous studies reported the oral administration of Naja naja atra venom (NNAV) reduced adriamycin-induced chronic kidney damage. This study investigated the effects of intragastric administrated cardiotoxin from Naja naja atra venom on chronic kidney disease in rats. Wistar rats were injected with

Purification, characterization and binding interactions of the Chinese-cobra (Naja naja atra) serum antitoxic protein CSAP.

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Ielogoties Reģistrēties

The characterization of the single-chain protein in Chinese-cobra (Naja naja atra) blood serum, which yields strong specific protection against the venom of the same snake, is reported. The protein, CSAP (cobra serum antitoxic protein), was purified to electrophoretic homogeneity. Over the pH range

Phospholipid hydrolysis and loss of membrane integrity following treatment of rat brain synaptosomes with beta-bungarotoxin, notexin, and Naja naja atra and Naja nigricollis phospholipase A2.

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Ielogoties Reģistrēties

The effects of the phospholipase A2 (PLA2) toxins, beta-bungarotoxin and notexin, and the PLA2 enzymes from Naja naja atra and Naja nigricollis snake venoms on the plasma membrane integrity of synaptosomes were examined. Synaptosomes were isolated from rat brain cerebral cortex, corpus striatum and

Study on camel IgG purification: a new approach to prepare Naja Naja Oxiana antivenom as passive immunization for therapy.

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Ielogoties Reģistrēties

A combined process of ammonium sulfate precipitation (salting out) and ion-exchange chromatography on DEAE-Sepharose CL-6B was used to prepare camel antivenom (IgG) against Naja Naja Oxiana for therapy. In the ammonium sulfate precipitation, the best condition for fractionation of IgG from the other

Some parameters of affinity chromatography in the purification of antibody against Naja naja siamensis toxin 3.

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Ielogoties Reģistrēties

Some parameters in the purification of antibodies against Naja naja siamensis toxin 3 by affinity chromatography were studied on toxin Sepharose, toxin-succinylaminoethyl Sepharose, toxin-albumin Sepharose and toxin succinylaminoethyl Biogel adsorbents. Immunologically pure antibody with 10-12-fold

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