Healthy

Watercress powder containing gluconasturtiin, which when added to a provided juice, will result in a target dose of up to 40 mg/day of 2-phenethyl isothiocyanate (PEITC). Preparation consumed at breakfast, lunch and dinner 3x daily for 2 weeks.

Watercress Preparation

Identical placebo powder added to a provided juice. Preparation consumed at breakfast, lunch and dinner 3x daily for 2 weeks.

Placebo Preparation

Clinical Trial of Watercress in Detoxification of Environmental Toxicants and Carcinogens

Prevalence of environment toxins in study population

Prevalence of carcinogens in study population

Masonic Cancer Center, University of Minnesota

National Cancer Institute (NCI)

Watercress will be tested in its natural form as a food item that will supplement the usual diet, via the prescription of watercress as whole food added daily to the usual diet. The intervention group will be asked to consume 100 grams of watercress per day, in addition to their usual diet for the total time of RT treatment. These 100 grams of watercress per day will allow the achievement of the daily "therapeutic" dose.

The control group will receive the standard of care, thus will maintain their ad libitum diet.

Long-term Effects Secondary to Cancer Therapy in Adults

100g of watercress daily during radiation therapy

Watercress

Hospital de Santa Maria

Does Watercress Intake Have an Impact on Cancer Patients Outcomes: a Longitudinal Trial

Does Watercress Intake Have an Impact on Cancer Patients Outcomes: a Randomized Longitudinal Trial

as an indirect measure of cell membrane integrity, function and metabolism

assessed by tetrapolar multifrequency Bioimpedance Analysis (BIA) and CT scan images; CT scans analysis will be performed in collaboration with expert groups

Assessed by the RTOG Common Toxicity Criteria, developed by the Eastern Cooperative Oncology Group (RTOG)

Evaluated by a radiation oncologist, using a validated toxicity scale

will be assessed by the European Organisation for Research and Treatment of Cancer (EORTC-QLQ C30), validated for Oncology

DNA damage assessment by Comet assay in lymphocytes has been used extensively as a surrogate biomarker to measure exposure to genotoxic agents and assess cancer risk in human studies by us and others. biological samples will comprise serum or plasma (1 mL) prepared from blood samples collected in Heparin tubes and urine (1 mL). Metabolomic profiles will be measured on a 700 MHz 1H NMR spectrometer equipped with a cryo-probe for enhanced sensitivity. 1H NMR spectroscopy: high throughput metabolic phenotyping is a powerful approach for characterising biochemical signatures of biological samples. We will couple high-resolution 1H NMR spectroscopy and mathematical modelling approaches to determine the metabolic profile in biological samples from patients.

Metabolomics simultaneously measures thousands of low molecular weight metabolites providing holistic information on the biochemical status of the body. Previous metabolomic studies have identified cancer-associated perturbations in the metabolic phenotypes of patients. This study will explore the ability of watercress to improve the restorative effects of radiotherapy in breast cancer patients on the metabolic status towards a healthy phenotype

assessed by the method Patient-Generated Subjective Global Assessment, specific and validated for Oncology

Will derive from a diet history and a 24-hour-recall food questionnaire. The software Dietplan6 (Forestfield Software Ltd 2013®, Horsham, UK) will analyze the nutrient content of foods consumed

The plasma samples analysis will also include the measurement of carotenoids and flavonoids, which are present in watercress, and are related with antigenotoxic effects in vitro and have anti-proliferative effects, as demonstrated in several studies. Urine samples analysis will allow the measurement of flavonoids.

assessed by tetrapolar multifrequency Bioimpedance Analysis (BIA)

Paula Ravasco

Principal Investigator

University of Lisbon

To measure the variation in extent of ITC excretion in urine from a capsule delivered dose of 100mg glucoraphanin

The extent of ITC excretion in urine from a capsule delivered dose of glucoraphanin

100mg glucoraphanin

Institute Of Food Research

The Conversion of ENcapsulated GlucorAphanin, Gut Microbiota Phylogeny and gEnotype Study (ENGAGE)

A Human Intervention Trial Investigating the Conversion of Encapsulated Glucoraphanin to Isothiocyanates; and the Potential Link Between the Extent of the Conversion to an Individual's Gut Microbiota Phylogeny and Genotype.

To measure urinary ITC by HP-LC and LC-MS using validated methods in urine collected during a dietary restriction period both before and after (24 hour urine collection) consumption of a 100mg glucoraphanin capsule.

To measure gut microbiota populations by faecal bacteria phylogenetic analysis.

To determine the genotype of participants for key polymorphic genes by PCR on DNA extracted from blood.

nasturtium/glutathione

Clinical Trial of Watercress in Detoxification of Environmental Toxicants and Carcinogens

Does Watercress Intake Have an Impact on Cancer Patients Outcomes: a Longitudinal Trial

The Conversion of ENcapsulated GlucorAphanin, Gut Microbiota Phylogeny and gEnotype Study (ENGAGE)

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