Lappuse 1 no 47 rezultātiem
Pseudomonas syringae pv. phaseolicola produces the tripeptide N delta(N'-sulfo-diaminophosphinyl)-ornithylalanyl-homoarginin e (phaseolotoxin), which functions as a chlorosis-inducing toxin in the bean halo blight disease by inhibiting ornithine carbamoyltransferase (OCT). The bacterium possesses
Floral bud calluses of Nicotiana tabacum L. var. Anand-2 produced multiple shoots on Murashige and Skoog's (MS) medium supplemented with 2 mg/l indole-3-acetic acid (IAA), 0.4 mg/l kinetin (Kn), and L-glutamic acid (2.5 mM). However cultures of calluses on MS medium containing only the IAA and Kn
The regulation of ornithine decarboxylase (ODC) expression was studied in suspension-cultured tobacco (Nicotiana tabacum L.) BY2 cells. ODC activity increased rapidly 3 h after cells re-entered the cell cycle from the stationary phase, corresponding to the G1 phase, and continued to increase in the
Nicotine synthesis was stimulated by reduction of the medium auxin concentration (induction medium) in callus tissue originating from Nicotiana tabacum cv. Samsun. The enzyme activities of the route ornithine to methylpyrroline, which are those of ornithine decarboxylase, putrescine
We studied the effects of dl-alpha-difluoromethylarginine (DFMA) and dl-alpha-difluoromethylornithine (DFMO), specific, irreversible inhibitors of arginine decarboxylase (ADC) and ornithine decarboxylase (ODC), respectively, on organogenesis growth and titers of free polyamines and conjugated
In an attempt to modulate the metabolism of polyamines in plants, Agrobacterium tumefaciens strains were produced which contained either a full-length or a 3'-truncated mouse ornithine decarboxylase (ODC) cDNA under the control of the cauliflower mosaic virus 35S promoter. Plants of Nicotiana
The Medicago truncatula ornithine aminotransferase cDNA was cloned under the potent constitutive 35S RNA promoter of the cauliflower mosaic virus and transferred into the genome of tobacco Nicotiana tabacum SR1 plants. Transformed tobacco plants grew better in salinity stress, but did not differ in
It is shown by tracer experiments with DL-[2-3H,5-14C]- and DL-[(RS)-5-3H,5-14C]rnithine, that the metabolic conversion of ornithine into proline, in three plant species (Nicotiana tabacum, Datura stramonium, and Lupinus angustifolius), takes place with maintenance of the delta-hydrogen atoms but
The cDNA encoding ornithine decarboxylase (ODC; EC 4.1.1.17), a key enzyme in putrescine and polyamine biosynthesis, has been cloned from Nicotiana glutinosa (GenBank AF 323910), and was expressed in Escherichia coli. The amino acid sequence of N. glutinosa ODC showed 90% identity with Datura
Transgenic plants of Nicotiana tabacum L. homozygous for an RNAi construct designed to silence ornithine decarboxylase (ODC) had significantly lower concentrations of nicotine and nornicotine, but significantly higher concentrations of anatabine, compared with vector-only controls. Silencing of ODC
Unlike most Nicotiana species, leaf tissues of the globally significant weed Nicotiana glauca Grah. (Argentinian tree tobacco) contains anabasine as the main component of its alkaloid pool, with concentrations typically increasing several fold in response to wounding of plants. The Δ(1)-piperidinium
Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive
Changes in the contents of polyamines (PAs) in tobacco leaves (Nicotiana tabacum L. cv. Wisconsin 38) grown under 16 h photoperiod were correlated with arginine and ornithine decarboxylase (EC 4.1.1.19 and EC 4.1.1.17) and diamine oxidase (EC 1.4.3.6) activities. The maximum of free and soluble
Non-host disease resistance involves the production of hypersensitive response (HR), a programmed cell death (PCD) that occurs at the site of pathogen infection. Plant mitochondrial reactive oxygen species (ROS) production and red-ox changes play a major role in regulating such cell death. Proline
In leaf and root tissues of Nicotiana tabacum L. (common tobacco), nicotine is by far the predominant pyridine alkaloid, with anatabine representing only a minor component of the total alkaloid fraction. The pyrrolidine ring of nicotine is derived from the diamine putrescine, which can be