setaria incrassata/phosphatase

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Acid phosphatase activity in microfilariae of Setaria labiato-papillosa and comparison with other blood microfilariae of dog and horse origin.

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Ielogoties Reģistrēties

Acid phosphatase activity was demonstrated in smears of Setaria labiato-papillosa microfilariae by the naphthol AS-TR-phosphate method. The staining was restricted to 3 distinct sites, corresponding to the excretory pore, the inner body and the anal pore. This staining pattern was compared with

MALDI mass sequencing and biochemical characterization of Setaria cervi protein tyrosine phosphatase.

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Ielogoties Reģistrēties

A 30-kDa acid phosphatase with protein tyrosine phosphatase activity was identified in Setaria cervi (ScPTP). The enzyme was purified to homogeneity using three-step column chromatography. Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis of purified ScPTP yielded a

Identification and characterization of novel membrane-bound PRL protein tyrosine phosphatases from Setaria cervi, a bovine filarial parasite.

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Ielogoties Reģistrēties

A significant amount of protein tyrosine phosphatase (PTP) activity was detected in the detergent-soluble membrane-bound fraction of Setaria cervi, a bovine filarial parasite. The membrane-bound PTP activity was significantly inhibited when the adult parasites were exposed to compounds having

Inhibition of Setaria cervi protein tyrosine phosphatases by Phenylarsine oxide: A proteomic and biochemical study.

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Ielogoties Reģistrēties

Phenylarsine oxide (PAO), a specific protein tyrosine phosphatase (PTP) inhibitor significantly decreased the motility and viability of Setaria cervi ultimately leading to its death. The PTP activity present in the cytosolic and detergent soluble fractions as well as on surface of these parasites

Presence of ecto-protein tyrosine phosphatase activity is vital for survival of Setaria cervi, a bovine filarial parasite.

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Ielogoties Reģistrēties

The ecto protein tyrosine phosphatases (PTP) are known to play a crucial role in the pathogenesis and survival of the intracellular parasites. However, their presence and role in filarial parasites is still unknown. We found a significant amount of tyrosine phosphatase activity in the surface

Setaria cervi dual specific phosphatase: characterization and its effect on eosinophil degranulation.

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Ielogoties Reģistrēties

Setaria cervi, a bovine filarial parasite contains significant acid phosphatase (AcP) activity in its various life stages. Two forms of AcP were separated from somatic extract of adult female parasite using cation exchange, gel filtration and concavalin affinity chromatography. One form having a

A comparison of the sites of acid phosphatase activity in an adult filaria, Setaria sp. and in some gastro-intestinal nematodes.

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Ielogoties Reģistrēties

The histochemical localization of acid phosphatase in an adult filaria, Setaria sp. obtained from the peritoneal cavity of a cow was closely examined and compared with that of adult nematodes parasitic in the host alimentary canal; special attention was paid to the intestine and body wall of the

Anthelmintic studies on Setaria cervi: histochemical alterations in glucose-6-phosphatase, adenosine triphosphatase and malic dehydrogenase.

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Ielogoties Reģistrēties

Histochemical enzyme pattern of microfilaria of Setaria cervi.

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Ielogoties Reģistrēties

The present study deals with the histochemical localization of glucose-6-phosphatase, malic dehydrogenase and aldolase in the microfilaria of Setaria cervi. Marked activity of glucose-6-phosphatase was observed in the cephalic cells, excretory and anal pores, G-cells and Innenkörper. Malic

Identification of a novel stress regulated FERM domain containing cytosolic protein having PTP activity in Setaria cervi, a bovine filarial parasite.

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Ielogoties Reģistrēties

A 67 kDa cytosolic FERM domain containing protein having significant protein tyrosine phosphatases activity (PTPL) has been purified to homogeneity from Setaria cervi, a bovine filarial parasite. The MALDI-MS/MS analysis of the purified protein revealed 16 peptide peaks showing nearest match to

Effect of anthelmintics on the enzyme activities of Setaria cervi (Nematoda: Filarioidea).

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Ielogoties Reģistrēties

The present study deals with the comparative efficacy of Hetrazan, levamisole and tetramisole on the enzyme activities of adult Setaria cervi worms. The drugs were administered orally to white rats, intraperitoneally infected with the bovine filariid, Setaria cervi. Biochemical assays revealed the

Setaria equina infection of Turkish equines: estimates of prevalence based on necropsy and the detection of microfilaraemia.

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Ielogoties Reģistrēties

Necropsies on 43 horses, 35 donkeys and two mules slaughtered in Ankara, Turkey, revealed that 12 (15%) of the equines harboured adult Setaria equina. When blood samples were checked for microfilariae, using Knott's method and a combination of membrane filtration followed by histochemical staining

Inheritance of some marker genes in Setaria italica (L.) P. Beauv.

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Ielogoties Reģistrēties

A study on a series of genetic markers was run on five hybrids of foxtail millet, Setaria italica, and on one interspecific hybrid S. viridisxS. italica (S. viridis is the wild relative of S. italica). Seven enzymatic systems were investigated using starch gel electrophoresis (esterase, alcohol

In vitro release of biologically active materials from the bovine filarial parasite Setaria digitata.

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Ielogoties Reģistrēties

Release of macromolecules by S. digitata, in 9 different media under in vitro condition have been studied. A direct relationship between microfilariae (mf) release and associated folin positive materials was seen in majority of the cases. High activities of hydrolytic enzymes such as protease,

Effect of anthelmintics and phenothiazines on adenosine 5'-triphosphatases of filarial parasite Setaria cervi.

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Ielogoties Reģistrēties

S. cervi showed particulate bound Ca2+ ATPase and Na+,K(+)-ATPase activities while Mg2+ ATPase was detected in traces. ATPase of S. cervi was also differentiated from the nonspecific p-nitrophenyl phosphatase activity. Female parasite and microfilariae exhibited higher Ca2+ ATPase and

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