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An extracellular proteolytic cascade promotes neuronal degeneration in the mouse hippocampus.

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Ielogoties Reģistrēties

Mice lacking the serine protease tissue plasminogen activator (tPA) are resistant to excitotoxin-mediated hippocampal neuronal degeneration. We have used genetic and cellular analyses to study the role of tPA in neuronal cell death. Mice deficient for the zymogen plasminogen, a known substrate for

Origin of acinar cell regeneration after atrophy of the rat parotid induced by duct obstruction.

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Ielogoties Reģistrēties

Acinar cell regeneration in the rat parotid gland after atrophy induced by a one week period of duct obstruction was examined using histology, immunohistochemistry and transmission electron microscopy (TEM). For immunohistochemistry, antibodies to 5-bromo-2'-deoxyuridine (BrdU), injected one hour

Sequential study of pancreatic structure and function during development of pancreatic acinar atrophy in a German shepherd dog.

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Ielogoties Reģistrēties

Sequential assessments of pancreatic structure and function were performed on a female German Shepherd Dog bred from parents with exocrine pancreatic insufficiency (EPI), to monitor development of pancreatic acinar atrophy in this breed. Determinations of serum trypsin-like immunoreactivity (TLI),

Juvenile pancreatic atrophy in Greyhounds: 12 cases (1995-2000).

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Ielogoties Reģistrēties

BACKGROUND This study describes compound failure of the endocrine and exocrine pancreas in Greyhounds, a condition that has not been reported in the veterinary literature. OBJECTIVE To describe the clinical and pathologic findings in 12 Greyhounds with juvenile pancreatic atrophy. METHODS Ten

Inspissation of pancreatic zymogen material in cystic fibrosis.

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Ielogoties Reģistrēties

Cystic fibrosis is characterized by the elaboration of abnormal, thick, tenacious mucus resulting in obstructive disease in sites such as the lung and pancreas. In the pancreas, acinar plugs of mucus have been reported as the earliest recognizable morphologic lesion in cystic fibrosis. Since mucus

Degradation of zymogen granules by lysosomes in cultured pancreatic explants.

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Ielogoties Reģistrēties

Pancreatic explants from Syrian hamsters were maintained in culture for determination of the fate of the zymogen granules in the acinar cells. By 6 hours of culture the zymogen granule matrix in many cells becomes nonhomogeneous, and electron-lucent spaces appear. Autoradiography performed 48 hours

Activation of prostate-specific antigen precursor (pro-PSA) by prostin, a novel human prostatic serine protease identified by degenerate PCR.

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Ielogoties Reģistrēties

A novel serine protease was found in human prostate by degenerate oligonucleotide PCR amplification and cloned. The zymogen form of this enzyme, named prostinogen, is composed of 240 amino acid residues with an amino-terminal propiece of 5 residues and a 235-residue mature enzyme. The transcript has

Pancreatic atrophy in copper-deficient rats: histochemical and ultrastructural evidence of a selective effect on acinar cells.

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Ielogoties Reģistrēties

Copper deficiency had a differential effect between tissues in the rat pancreas. There was marked loss and atrophy of acinar cells, in which both hypertrophied and degenerating mitochondria were present. Cytochrome oxidase activity in acini was greatly depleted while monoamine oxidase activity was

Autophagy impairment in pancreatic acinar cells causes zymogen granule accumulation and pancreatitis.

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Ielogoties Reģistrēties

Basal autophagy degrades many kinds of proteins and organelles to maintain quality and plays important roles in cellular homeostasis. However, the impact of basal autophagy on zymogen granules in pancreatic acinar cells is unknown. In the present study, we examined the influence of autophagy

Loss of Sbds in zebrafish leads to neutropenia and pancreas and liver atrophy

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Ielogoties Reģistrēties

Shwachman-Diamond syndrome (SDS) is characterized by exocrine pancreatic insufficiency, neutropenia, and skeletal abnormalities. Biallelic mutations in SBDS, which encodes a ribosome maturation factor, are found in 90% of SDS cases. Sbds-/- mice are embryonic lethal. Using CRISPR/Cas9 editing, we

Identification and cloning of GP-3 from rat pancreatic acinar zymogen granules as a glycosylated membrane-associated lipase.

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Ielogoties Reģistrēties

The protein components of highly purified secretory granule membranes and the granule contents from rat exocrine pancreas were characterized by two-dimensional polyacrylamide gel electrophoresis, protein staining, lectin absorption, and Western blotting with anti-secretory protein antibodies.

The degenerative changes in pancreatic acinar cells caused by DL-ethionine.

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Ielogoties Reģistrēties

Degeneration of pancreatic acinar cells in rats injected with ethionine was studied by electron microscopy. The most conspicuous morphologic lesions occurred in the ergastoplasm. There was a widening of the endoplasmic reticulum, a decrease in number of membrane-associated ribosomes, and a

The effect of antecedent acute ethanol ingestion on the pancreas ultrastructure in taurocholate pancreatitis in rats.

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Ielogoties Reģistrēties

The rat pancreas ultrastructure was examined 6, 12, and 18 h after (1) taurocholate-induced acute pancreatitis and after (2) pancreatitis preceded 6 h earlier by intragastric acute 40% ethanol ingestion (5 g/kg b.w.). Pancreatic specific trypsin activity and plasma alpha-amylase were assayed at the

Morphometric and fine-structural studies of rat pancreatic acinar cells during early postnatal life.

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Ielogoties Reģistrēties

Pancreatic acinar cells of rats obtained at 1, 2, 3, 5, 7 and 14 days of age were examined using fine structural and morphometric techniques. From 5 days of age onwards, the acinar cells were analysed twice per day, at 20.00 h and 08.00 h. The present study demonstrates changes in the average volume

Effects on endogenous acetaldehyde production by disulfiram and ethanol feeding on rat pancreas.

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Ielogoties Reģistrēties

Exogenous acetaldehyde infusion can induce pancreatitis-like injury of the pancreas in some isolated pancreas models, whereas in vivo such treatment has failed to induce pancreatitis. In vivo exogenous acetaldehyde may not be effective because it is rapidly metabolized. The aim of this study was to

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