TNF-muteins

BACKGROUND OF THE INVENTION

Tumor Necrosis Factor, or more specifically Tumor Necrosis Factor-alpha (TNF-.alpha.), is a cytokine, primarily produced by stimulated macrophages, that exhibits not only a striking cytotoxicity against various rumour cells [Carswell et al., Proc. Nat. Acad. Sci., U.S.A. 72, 3666-3670, (1975)] but also plays a multiple role as a mediator of inflammation and the immune response [for an overview see Beutler and Cerami, Ann. Rev. Immunol. 7, 625-655 (1989); Bonavista and Granger (eds.) "Tumor Necrosis Factor: Structure, Mechanism of Action, Role in Disease and Therapy, Karger, Basel (1990)]. The primary structure of human Tumor Necrosis Factor-alpha (hTNF-.alpha.) has been deduced from the nucleotide sequence of a eDNA which has been cloned and expressed in E. coli [Pennica et al., Nature 312, 724-729 (1984); Marmenout et al., Eur. J. Biochem. 152, 515-522 (1985); Wang et al., Science 228, 149-154 (1985); Shirai et al., Nature 313, 803-806 (1985)]. A striking homology in amino acid sequence (30%) was found between hTNF-.alpha. and human Lymphotoxin, often referred to as human Tumor Necrosis Factor-beta (hTNF-.beta.), a cytokine mainly produced by lymphocytes [Gray et al., Nature 312, 721-724 (1984); Fiers et al., Cold Spring Harbour Symp. 51, 587-595 (1986)].

h TNF-.alpha. with modified amino acid sequences, so called TNF-.alpha.-muteins, have also been described in the art [for example see Yamagishi et al., Protein Engineering 3, 713-719, (1990) or Fiers in "Tumor Necrosis Factors: Structure, Function and Mechanism of Action", Aggarwal and Vilcek (eds.), Marcel Dekker, Inc., New York, in press, or Fiers et al. in Bonavista and Granger, pp. 77-81 (s.a.)]. In addition TNF-.alpha.-muteins have also been the object of several patent applications, e.g. International Patent Applications Publ. Nos. WO 86/02381, WO 86/04606, WO 88/06625 and European Patent Applications Publ. Nos. 155,549; 158,286; 168,214; 251,037 and 340,333, and Deutsche Offenlegungsschrift Nr. 3843534.

Muteins of Lymphotoxin have also been disclosed in the art, e.g. in European Patent Applications Publ. Nos. 250,000; 314,094 and 336,383.

The biological effects of TNF are mediated via specific receptors, namely a receptor with an apparent molecular weight of 55 kD on sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) (p55-TNF-R) and a receptor with an apparent molecular weight of 75 kD on SDS-PAGE (p75-TNF-R). Both forms of TNF-receptor have been cloned, p55-TNF-R by Loetscher et al. [Cell 61, 351-359, (1990)] and p75-TNF-R by Dembic et al. [Cytokine 2, 53-58, (1990)] (for both receptors see also European Patent Application No. 90116707.2). It was recently found that both receptors bind not only TNF-.alpha. but also TNF-.beta. with high affinity [Schonfeld et al., J. Biol. Chem. 266, 3863-3869 (1991)].

It is well known in the art that on the basis of its biological activities TNF-.alpha. can be a valuable compound for the treatment of various disorders. For example TNF-.alpha., alone or in combination with interferon, can be an effective antitumor agent [Brouckaert et al., Int. J. Cancer 38, 763-769 (1986)]. However, its systemic toxicity is a limit to its wider therapeutic use [Taguchi T. and Sohmura Y., Biotherapy 3, 177-186 (1991)].

It has been shown that human TNF-.alpha. (hTNF-.alpha.) in mice only binds to the 55 kD mouse TNF receptor (murine p55-TNF-R) and is far less toxic than murine TNF-.alpha. (mTNF-.alpha.), which binds to both p55-TNF-R and p75-TNF-R. In C57B16 mice, the LD50 of mTNF.alpha. is about 10 .mu.g/mouse and the LD50 of hTNF-.alpha. is about 500 .mu.g/mouse [Brouckaert et al., Agents and Actions 26, 196-198 (1989); Everaerdt, B. et al., Biochem. Biophys. Res. Comm. 163, 378-385 (1989); Lewis, M. et al., Proc. Natl. Acad. Sci. USA 88, 2830 (1991)]. Hence the p75-TNF-R seems to play a special role in systemic toxicity.

hTNF-.alpha. and mTNF-.alpha. bind almost equally well to human p55-TNF-R (hp55-TNF-R) and to human p75-TNF-R (hp75-TNF-R). However, hTNF-.alpha. mutants, which have retained the biological activity mediated by hp55-TNF-R but have lost nearly all activity mediated by hp75-TNF-R, are the functional equivalent of hTNF-.alpha. in the murine system in that they are expected to have reduced systemic toxicity in primates just as hTNF-.alpha.. has reduced toxicity in mice and fails to bind mouse p75-TNF-R.

Human Tumor Necrosis Factor muteins showing a significant difference between their binding affinity to the human p75-Tumor-Necrosis-Factor-Receptor (hp75-TNF-R) and to the human p55-Tumor-Necrosis-Factor-Receptor (hp55-TNF-R), have been described in European Patent No. 486 908.

SUMMARY OF THE INVENTION

This invention is directed to hTNF muteins or pharmaceutically acceptable salts thereof having the amino acid sequence of human Tumor Necrosis Factor but substituted at least at position 86 with another amino acid (having at position 86 a threonine instead of a serine residue), which retain ability bind to hp55-TNF-R, but have substantially lost ability to bind hp75-TNF-R. This invention is also directed to hTNF muteins which retain biological activity mediated by hp55-TNF-R, while no longer binding to hp75-TNF-R. However, the hTNF muteins of the present invention are not restricted to this type of mutein. Muteins of another type binding exclusively to hp55-TNF-R but having lost the capacity to elicit a functional cell response are also included.

In accordance with this invention the hTNF muteins by virtue of substitution at least at position 86 bind more strongly to hp55-TNF-R than to hp75-TNF-R.

BRIEF DESCRIPTION OF THE FIGURES

The following abbreviations and symbols used are: B, E, H, S, Xb and X which indicate cleavage sites for restriction enzymes B glI, EcoRI, HindlII, SalI, XbaI and XhoI, respectively. represents the regulatable promoter/operator element N25OPSN25OP29, represents the synthetic ribosomal binding site RBSII,SphI, represents genes for TNF.alpha. (TNF.alpha.), .beta.-lactamase (bla), chloramphenicol acetyltransferase (cat), lac repressor (lacI) and neomycin phosphotransferase (neo), represents transcriptional terminators t.sub.o of phage lambda (t.sub.o) and T1 of the E. coli rrnB operon (T1).revreaction. represents the replication regions of plasmids pBR322 and pREP4 (repl.),.fwdarw. represents the coding region under control of N25

OPSN25OP29 and RBSII,SphI.

FIG. 1a is a schematic drawing of the plasmid pDS56/RBSII,SphI-TNF.alpha..

FIGS. 1b-1 through 1b-4 displays the complete nucleotide sequence of plasmid pDS56/RBSII,SphI-TNF.alpha.. In this sequence, the recognition sequences of the restriction enzymes depicted in FIG. 1a are indicated. The amino acid sequence shown represents in the three letter code the sequence of the mature TNF.alpha. (157 amino acids). [SEQ ID: 1]

FIG. 2a is a schematic drawing of the plasmid pREP4.

FIGS. 2b-1 through 2b-3 displays the complete nucleotide sequence of plasmid pREP4. In this sequence, the recognition sequences of the restriction enzymes depicted in FIG. 2a are indicated. [SEQ ID: 3]

FIG. 3 outlines the preparation of an EcoRI-HindlII fragment encoding the TNF.alpha. mutein Thr.sup.86 -TNF.alpha..

FIG. 4 displays the nucleotide sequence of Fragment 1 of plasmid pDS56/RB SII,SphI-INF.alpha.(Trp32) [SEQ ID: 4]

FIG. 5 displays the nucleotide sequence of Fragment 1 of plasmid "pDS56/RBSII,SphI-TNF.alpha.(Ser29) [SEQ ID: 6]

FIG. 6 displays the nucleotide sequence of Fragment 1 of plasmid pDS56/RBSII,SphI-TNF.alpha.(Ser29Trp32) [SEQ ID: 8]

FIGS. 7A and 7B Competitive binding of wild-type human TNF.alpha. and Thr.sup.86, Trp.sup.32 -Thr.sup.86 and Ser.sup.29 -Trp.sup.32 -Thr.sup.86 muteins to recombinant human p-75 and p-55 TNF-R's.

FIG. 7b Microtiter plates coated with recombinant human p-75TNF-R-IgG.gamma.3 fusion protein (FIG. 7A) and recombinant human p-55TNF-R-IgG.gamma.3 fusion protein (FIG. 7B) were incubated with radiolabelled human TNF.alpha. in the presence of different concentrations of wild-type TNF.alpha. (closed circles), Thr.sup.86 mutein (open circles), Trp.sup.32 -Thr.sup.86 mutein (open squares) and Ser.sup.29 -Trp.sup.32 -Thr.sup.86 mutein (open triangles). After three hours at room temperature bound radioactivity was counted in a .gamma.-counter.

FIGS. 8A and 8B Competitive binding of wild-type human TNF.alpha. and Ser.sup.29 -Thr.sup.86 Glu.sup.31 -Thr.sup.86 and Asn.sup.31 -Thr.sup.32 -Thr.sup.86 muteins to recombinant human p-75 and p-55TNF-R's.

FIG. 8b Microtiter plates coated with recombinant human p-75TNF-R-IgG.gamma.3 fusion protein (FIG. 8A) and recombinant human p-55TNF-R-IgG.gamma.3 fusion protein (FIG. 8B) were incubated with radiolabelled human TNF.alpha. in the presence of different concentrations of wild-type TNF.alpha. (closed circles), Ser.sup.29 -Thr.sup.86 mutein (open circles), Asn.sup.31 -Thr.sup.32 -Thr.sup.86 mutein (open squares) and Glu.sup.31 -Thr.sup.86 mutein (open triangles). After three hours at room temperature bound radioactivity was counted in a .gamma.-counter.

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides human Tumor Necrosis Factor muteins or pharmaceutically acceptable salts thereof, having selective binding affinity for the p55 subunit of human Tumor-Necrosis-Factor-Receptor (hp55-TNF-R) characterized in that the amino acid sequence of human Tumor Necrosis Factor is changed at least at position 86, having a threonine instead of a serine residue at this position. By selective binding is meant that the muteins of this invention bind to the p55 subunit (hp55-TNF-R) without any substantial binding to the p75 subunit (hp75-TNF-R).

The amino acid sequence of human TNF-.alpha. as disclosed by Pennica et al. [s.a.] is as follows:

__________________________________________________________________________ 1 10 VAL ARG SER SER SER ARG THR PRO SER ASP LYS PRO VAL ALA HIS 20 29 30 VAL VAL ALA ASN PRO GLN ALA GLU GLY GLN LEU GLN TRP LEU ASN 31 32 40 [SEQ ID: 2] ARG ARG ALA ASN ALA LEU LEU ALA ASN GLY VAL GLU LEU ARG ASP 50 60 ASN GLN LEU VAL VAL PRO SER GLU GLY LEU TYR LEU ILE TYR SER 70 GLN VAL LEU PHE LYS GLY GLN GLY CYS PRO SER THR HIS VAL LEU 80 86 90 LEU THR HIS THR ILE SER ARG ILE ALA VAL SER TYR GLN THR LYS 100 VAL ASN LEU LEU SER ALA ILE LYS SER PRO CYS GLN ARG GLU THR 110 120 PRO GLU GLY ALA GLU ALA LYS PRO TRP TYR GLU PRO ILE TYR LEU 130 GLY GLY VAL PHE GLN LEU GLU LYS GLY ASP ARG LEU SER ALA GLU 140 150 ILE ASN ARG PRO ASP TYR LEU ASP PHE ALA GLU SER GLY GLN VAL 157 TYR PHE GLY ILE ILE ALA LEU __________________________________________________________________________

(also disclosed by Marmenout et al. (s.a.) or Wang et al. (s.a.) or Shirai et al.) or more specifically as coded for by the nucleotide sequence of the insert of the plasmid pDS56/RBSII,SphI-TNF.alpha. (see FIGS. 1a and 1b and Example I), which insert sequence codes for mature TNF-.alpha..

The hTNF muteins as defined above may have amino acids that differ from the amino acids at the same positions in hTNF at one or more positions in addition to 86, preferably at one or two additional positions, whereby positions 29, 31, 32, 29 and 32, or 31 and 32 are especially preferred individually or in any combination. Any amino acid, preferably any naturally occurring one, can be used at these additional positions. The amino acid sequence of these preferred hTNF muteins is as follows:

__________________________________________________________________________ 1 10 VAL ARG SER SER SER ARG THR PRO SER ASP LYS PRO VAL ALA HIS 20 29 30 VAL VAL ALA ASN PRO GLN ALA GLU GLY GLN LEU GLN TRP X.sub.1 ASN 31 32 40 X.sub.2 X.sub.3 ALA ASN ALA LEU LEU ALA ASN GLY VAL GLU LEU ARG ASP 50 60 ASN GLN LEU VAL VAL PRO SER GLU GLY LEU TYR LEU ILE TYR SER 70 GLN VAL LEU PHE LYS GLY GLN GLY CYS PRO SER THR HIS VAL LEU 80 86 90 LEU THR HIS THR ILE SER ARG ILE ALA VAL THR TYR GLN THR LYS 100 VAL ASN LEU LEU SER ALA ILE LYS SER PRO CYS GLN ARG GLU THR 110 120 PRO GLU GLY ALA GLU ALA LYS PRO TRP TYR GLU PRO ILE TYR LEU 130 GLY GLY VAL PHE GLN LEU GLU LYS GLY ASP ARG LEU SER ALA GLU 140 150 ILE ASN ARG PRO ASP TYR LEU ASP PHE ALA GLU SER GLY GLN VAL 157 TYR PHE GLY ILE ILE ALA LEU [SEQ ID: 17], where __________________________________________________________________________

X.sub.1, X.sub.2, and X.sub.3 represent any amino acid. Therefore, in the muteins of this invention position 29 may be Leu while 30 and 32 are X, or position 29 may be X, while position 30 is Arg and 32 is X, or any other combination of wild-type amino acids or substituted amino acids at these three positions.

In the muteins of this invention, for substitutions at position 29 serine, glycine or tyrosine are preferred and serine is especially preferred. For substitutions at position 31 glutamic acid or asparagine are preferred. For substitutions at position 32 tyrosine, tryptophan or threonine are preferred, and tryptophan and threonine are specifically preferred.

Especially preferred hTNF muteins of the present invention are Thr.sup.86 -TNF-.alpha., Ser.sup.29 -Thr.sup.86 -TNF-60 , Glu.sup.31 -Thr.sup.86 -TNF-.alpha., Trp.sup.32 -Thr.sup.86 -TNF-.alpha., Ser.sup.29 -Trp.sup.32 -Thr.sup.86 -TNF-.alpha. or Asn.sup.31 -Thr.sup.32 -Thr.sup.86 -TNF-.alpha..

The hTNF muteins of the present invention may contain further amino acid substitutions if such substitutions do not alter their selective binding affinity for the p55-TNF-R. Amino acid substitutions in proteins and polypeptides which do not essentially alter biological activity are known in the art and described, e.g. by H. Neurath and R. L. Hill in "The Proteins", Academic Press, New York (1979), in particular in FIG. 6 of page 14. The most frequently observed amino acid substitutions are Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr, Ser/Asn, Ala/Val, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile, Leu/Val, Ala/Glu, Asp/Gly and vice versa. The hTNF muteins of the present invention may additionally contain sequences of several amino acids which are coded for by "linker" sequences. These sequences may come from the expression vectors used for expression of the hTNF muteins of the invention.

The hTNF muteins of the present invention can also contain specific sequences that preferably bind to an affinity carrier material. Examples of such sequences are sequences containing at least two adjacent histidine residues (see in this respect European Patent Publication No. 282 042). Such sequences bind selectively to nitrilotriacetic acid nickel chelate resins (Hochuli and Dobeli, Biol. Chem. Hoppe-Seyler 368, 748 (1987); European Patent Publication No. 253 303). hTNF muteins which contain such a specific sequence can be linked either to the C-terminus or the N-terminus, or to both termini, of the hTNF-mutein amino acid sequences.

The hTNF muteins of the present invention can also be combined with different immunoglobulin heavy chain or light chain polypeptides. This leads to chimaeric hTNF mutein immunoglobulin polypeptides which could have increased half-life in vivo. Increased half-life in vivo has been shown for chimetic polypeptides consisting of the first two domains of the constant regions of the heavy chain or the light chain of a mammalian immunoglobulin (see Traunecker et al., Nature 331, 84-86 [1988] and European Patent Publication No. 394 827).

The hTNF muteins can also be coupled to polymers such as polyethylene glycol or polypropylene glycol having a molecular weight of 500 to 20,000 daltons. This leads to protected hTNF mutein compositions which could be substantially non-immunogenic. Several modes of coupling the polymer with the polypeptide are available and described, e.g., in U.S. Pat. No. 4,179,337.

The hTNF muteins of the present invention can be produced by recombinant methods known in the art and described e.g. in Sambrook et al. [Molecular Cloning, A Laboratory Manual, 2nd ed., Cold Spring Harbour Laboratory, Cold Spring Harbour Laboratory Press, USA (1989)] and in the following paragraphs. Assays to determine the selective binding affinity for p55-TNF-R of such hTNF muteins are described below and in the Examples. Alternatively, the muteins of the present invention can be chemically synthesized using standard methods known in the art, preferably solid state methods, such as the methods of Merrifield (J. Am. Chem. Soc. 85, 2149-2154 [1963]). Salts of such muteins are also an object of the present invention. Such salts can be produced by methods known in the art.

Separating beneficial from unwanted TNF-.alpha. activities by using compounds specifically binding to one or the other TNF-receptor, such as the hTNF muteins of the present invention, can be used in general in any disease states where TNF plays a role as described below.

Polynucleotides comprising a coding DNA sequence coding for hTNF-muteins as hereinbefore described or comprising DNA sequences which are complementary to such coding sequences are also an object of the present invention. Such DNA sequences can be constructed starting from genomic or cDNA sequences coding for hTNF as disclosed in the art [s.a.] using known methods of in vitro mutagenesis [see e.g. Sambrook et al., 1989]. Preferred polynucleotides comprise DNA sequences coding for proteins comprising the amino acid sequence of hTNF-.alpha. but with Thr at position 86 (Thr.sup.86 TNF-.alpha.), or additionally with Gly, Set, or Tyr at position 29, preferably Ser (Ser.sup.29 Thr.sup.86 TNF-.alpha.) or with Glu or Asn at position 31, preferably Glu (Glu.sup.31 Thr.sup.86 TNF-.alpha.), or with Tyr, Trp or Thr at position 32, preferably Trp or Thr, most preferably Trp (Trp.sup.29 Thr.sup.86 TNF-.alpha.), or with Ser at position 29 and Trp at position 32 (Ser.sup.29 Trp.sup.32 Thr.sup.86 TNF-.alpha.), or with Asn at position 31 and Thr at position 32 (Asn.sup.31 Thr.sup.32 Thr.sup.86 TNF-.alpha.), and the DNA sequences complementary to such sequences. DNA sequences of this invention include DNA sequences coding for an hTNF mutein with selective binding affinity for hp55-TNF-R, which mutein comprises the sequence of hTNF.alpha. with a Thr at position 86 instead of a Set, and additional substitutions which do not alter the selective binding properties of the mutein encoded.

The DNA sequences coding for the proteins are useful as intermediates in the production of said proteins, as further described below.

DNA sequences of this invention may be obtained from vectors containing those sequences as described herein, or from vectors containing the DNA sequence of hTNF-.alpha. as disclosed in the art which are mutagenized as described below. Alternatively, such DNA sequences may be derived using the genetic code from the amino acid sequences of the proteins of this invention and chemically synthesized by methods known in the art.

Such mutagenesis can be carried out at random in order to obtain a large number of mutants which can then be tested for their desired properties in appropriate assay systems. Alternatively, defined positions in a given DNA sequence can be mutated by so called site directed mutagenesis [see e.g. Sambrook et al., 1989, 15.51-15.113] or by mutagenesis using the polymerase chain reaction [see e.g. White et al., Trends in Genetics 5, 185-189 (1989)].

One chemical mutagen which is often used for random mutagenesis is sodium bisulfite, which converts a cytosine residue into an uracil residue and hence leads to a transition of "C" to "T" (standard abbreviations for nucleotides) [for the method see e.g. Shortle and Nathans, Proc. Nat. Acad. Sci. U.S.A. 75, 2170-2174 (1978) or Pine and Huang, Meth. Enzym. 154, 415-430 (1987)]. This mutagen acts solely on single stranded DNA. However, the mutated target DNA sequence is expressed in a double stranded plasmid vector. To avoid the necessity of recloning from mutagenesis to expression vectors, so called "phasmids" may be used. These are vectors which, in addition to a plasmid origin of replication, carry an origin of replication derived from a filamentous phage. Examples of such phasmids are the pMa- and pMc-phasmids as described by Stanssen et al. [Nucleic Acids Res. 17, 4441-4454, (1989)]. Using this expression system one can construct so called "gap-duplex"-structures [see also Kramer et al., Nucl. Acids. Res. 12, 9441-9456 (1984)] where only the TNF-coding sequence (s.a.) is in a single stranded configuration and therefore accessible to the specific chemical mutagen. "Gap-duplexes" to be used in random mutagenesis can be constructed as described for site-specific mutagenesis by Stanssen et al. [s.a.] that the (-)strand contains the same active antibiotic resistance gene as the (+)strand. By making use of different restriction sites in the DNA-sequence encoding hTNF.alpha., variation of the width of the gap is possible. Examples of such restriction sites are the C1a1-Sal1 sites (470 nucleotides), BstX1--BstX1 sites (237 nucleotides) or Styl-Sty1 sites (68 nucleotides). Such gap-duplex-constructs can then be treated with increasing concentrations (up to 4M) of bisulfite, followed by several dialysis steps, as described by Shortle and Nathans (s.a.). A suitable procaryotic host cell can then be transformed by such phasmid constructs according to methods known in the art and described e.g. by Sambrook et al. (s.a.). A suitable procaryotic host cell means in this context a host cell deficient in a repair function so that a mutated residue is maintained in the DNA during replication and which host cell is capable of expressing the corresponding mutated TNF. For example, the host cell may be deficient in a specific repair function such that a uracil residue is maintained as a mutation. Such specific host strains are known in the art, for example for E. coli strains, e.g. E. coli BW 313 [Kunkel, T. A., Proc. Natl. Acad. Sci. USA 82, 488-492 (1985)]. The resulting clones can then be screened for those expressing a desired hTNF mutein by appropriate assay systems as described below. For example each colony can be inoculated in a microtiter plate in a suitable medium containing the relevant antibiotic. The cells may be lysed by addition of lysozyme, followed by sequential freeze-thaw cycles. After precipitation of nucleic acids and centrifugation, the supernatant of each colony can directly be used in appropriate assays as described, e.g., in Example IIa and IIb or Example VIII measuring binding to the p75-TNF-R and the p55-TNF-R on the surface of living cells or in purified form.

If desired, the specific sites of mutation can be determined, for example by restriction fragment analysis [see e.g. Sambrook et al. (s.a.)]. By determination of the DNA sequence of such fragments the exact position of the mutation can be determined and if such mutation leads to an amino acid replacement the new amino acid can be deduced from the determined DNA sequence. DNA sequencing can be performed according to methods known in the art, for example, by using T7 polymerase on supercoiled DNA with a commercially available sequencing kit (Pharmacia, Uppsala, Sweden).

As already mentioned above, another possibility for mutating a given DNA sequence is by "site directed mutagenesis". A widely used strategy for such mutagenesis as originally outlined by Hutchinson and Edgell [J. Virol. 8, 181 (1971)] involves the annealing of a synthetic oligonucleotide carrying the desired nucleotide substitution to a target region of a single-stranded DNA sequence wherein the mutation should be introduced [for review see Smith, Annual. Rev. Genet. 19, 423 (1985) and for improved methods see references 2-6 in Stanssen et al. (1989)].

One preferred method is that of Stanssen et al. (1989) using "gapped duplex DNA" as originally described by Kramer et al. (1984) [see above and Kramer and Fritz, Methods in Enzymology, (1987), Academic Press, Inc., USA] but using antibiotic resistance genes instead of M13 functional genes for selection of the mutated strand in addition to the phasmid technology as also described by Stanssen et al. (1989) [s.a.]. An advantage of this method is that successive cycles of mutagenesis can be performed without the need to transfer the gene to a new mutagenesis vector: second round mutagenesis differs only in the selection to another antibiotic marker (Stanssen et al., s.a.). As a control, site-specific back mutagenesis of the mutant to the wild-type TNF can be used. In addition, the use of an oligonucleotide to create or destroy a restriction site in the TNF gene, allows control of the mutant not only by hybridization to the oligonucleotide used for site directed mutagenesis but also by the presence or absence of the restriction site. In order to create a set of hTNF muteins with wild-type amino acids replaced by any naturally occurring amino acid at a defined position of their amino acid sequence a set of oligonucleotides is used with all possible codons at the defined position.

As mentioned above, another possibility of mutating a given DNA sequence is mutagenesis by using the polymerase chain reaction (PCR). The principles of this method are outlined by White et al. (1989), and improved methods are described in Innis et al. [PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc. (1990)].

PCR is an in vitro method for ,producing large amounts of a specific DNA fragment of defined length and sequence from small amounts of a template DNA. PCR is based on the enzymatic amplification of the DNA fragment which is flanked by two oligonucleotide primers that hybridize to opposite strands of the target sequence. The primers are oriented with their 3' ends pointing towards each other. Repeated cycles of heat denaturation of the template, annealing of the primers to their complementary sequences and extension of the annealed primers with a DNA polymerase result in the amplification of the segment between the 5' ends of the PCR primers. Since the extension product of each primer can serve as a template for the other, each cycle essentially doubles the s amount of the DNA fragment produced in the previous cycle. Since the primers are physically incorporated into the amplified product and mismatches between the 5' end of the primer and the template do not significantly affect the efficiency of the amplification, it is possible to alter the amplified sequence thereby introducing the desired mutation into the amplified DNA. By utilizing the thermostable Taq DNA polymerase isolated from the thermophilic bacteria Thermus aquaticus, it has been possible to avoid denaturation of the polymerase which necessitated the addition of enzyme after each heat denaturation step. This development has led to the automation of PCR by a variety of simple temperature-cycling devices. In addition, the specificity of the amplification reaction is increased by allowing the use of higher temperatures for primer annealing and extension. The increased specificity improves the overall yield of amplified products by minimizing the competition by non-target fragments for enzyme and primers.

Design and synthesis of oligonucleotides can be effected as known in the art and described e.g. in Sambrook et al. (1989) or in one of the references cited above with respect to site directed mutagenesis.

As soon as a DNA sequence coding for a hTNF-mutein of the present invention has been created, expression can be effected by the phasmid technology as described above or by use of any suitable proor eukaryotic expression system well known in the art [see e.g. Sambrook et al., s.a.].

Expression is effected preferably in prokaryotic cells, e.g., in E. coli, Bacillus subtilis and so on, whereby E. coli, specifically E. coli K12 strains e.g. M15 [described as DZ 291 by Villarejo et al. in J. Bacteriol. 120, 466-474 (1974)], HB 101 [ATCC No. 33694], WK6 (Stranssens et al. s.a.) or E. coli SG13009 [Gottesman et al., J. Bacteriol. 148, 265-273 (1981)] are preferred. Expression of the hTNF muteins of the present invention can also be effected in lower or higher eukaryotic cells, for example yeast cells (Saccharomyces, Pichia etc.), filamentous fungi (Aspergillus etc.) or cell lines (chinese hamster ovary cell lines etc.). Expression in yeast cells is preferred [see Sreekrishna et al., Biochem. 28, 4117-4125, (1989); Hitzeman et al., Nature 293, 717-722 (1981); European Patent Publication No. 263 311]. Expression of the hTNF muteins of the present invention may occur in such systems either intracellularly, or, after suitable adaption of the gene, extracellularly (see Leemans et al., Gene 85, 99-108, 1989).

Suitable vectors used for expression in E. Coli are mentioned by Sambrook et al. [s.a.] or by Fiers et al. in "Proc. 8th Int. Biotechnology Symposium" [Soc. Franc. de Microbiol., Pads, (Durand et al., eds.), pp. 680-697 (1988)] and more specifically vectors of the pDS family [Bujard et al., Methods in Enzymology, eds. Wu and Grossmann, Academic Press, Inc. Vol. 155, 416-433 (1987); Stuber et al., Immunological Methods, eds. Lefkovits and Pernis, Academic Press, Inc., Vol. IV, 121-152 (1990)] for example pDS56/RBSII,SphI-TNF.alpha.(Thr86) (see Example I) or pDS56/RBSII,SphI-TNF.alpha.(Trp32Thr86) (see Example III) or pDS56/RBSII,SphI-TNF60 (Ser29Thr86) or pDS56/RBSII,SphI-TNF.alpha.(Ser29Trp32Thr86) or pDS56/RBSII,SphI-TNF.alpha.(Asn31Thr32Thr86) or pDS56/RBSII,SphI-TNF.alpha.(Glu31Thr86) (see Example IV). Since these specific pDS56/RBSII-plasmids achieve a high level of expression due to their specific regulatable promoter/operator elements and ribosomal binding sites the plasmids can be maintained in E. coli cells only when the activity of the promoter/operator element is repressed by the binding of a lac repressor to the operator. The activity of the promoter can be restored at the desired cell density by addition of IPTG, which inactivates the repressor and clears the promoter. Since most of the E. coli strains do not provide enough repressor molecules to completely repress the function of the promoter sequences present in these high copy number plasmids, such E. coli strains, like E. coli M15 or SG13009, have to be transformed at first with a plasmid, like pREP 4 (see FIGS. 2a and b), coding for the lac repressor, before being transformed with the specific pDS56/RBSII-plasmids of the invention which can then be stably maintained in the E. coli cells. Beside coding for the lac repressor, pREP4 contains also a region of the plasmid pACYC184 [Chang and Cohen, J. Bacteriol. 134, 1141-1156 (1978)], which contains all information required for replication and stable transmission to daughter cells [for additional information see also "System for high level production in E. coli and rapid purification of recombinant proteins: application to epitope mapping, preparation of antibodies and structure function analysis" by Stuber et al. in Immunological Methods, Vol. IV, pp 121-152, Lefkovits and Pernis (eds.), Academic Press, New York (1990)].

Transformation of the host cells by vectors as described above may be carried out by any conventional procedure [see for example Sambrook et al. (s.a.)]. Where the host cell is a prokaryote such as E. coli, competent cells which are capable of DNA uptake are prepared from cells harvested after exponential growth phase and subsequently treated according to the known CaCl.sub.2 -method. Transformation can also be performed after forming a protoplast of the host cell or by other methods known in the art and described, e.g., in Sambrook et al. (s.a.). Therefore a vector, especially for expression in a prokaryotic or lower eukaryotic host cell, comprising a DNA sequence coding for an hTNF mutein as described above, and a host cell, especially a prokaryotic host cell, e.g. E. coli, or a lower eukaryotic host cell, transformed by such a vector are also an object of the present invention.

Usually, the host organisms which contain a desired expression vector are grown under conditions which are optimal for their growth. In case of a procaryotic host at the end of the exponential growth, when the increase in cell number per unit time decreases, the expression of the desired hTNF mutein is induced, i.e. the DNA coding for the desired hTNF mutein is transcribed and the transcribed mRNA is translated. The induction can be carried out by adding an inducer or a derepressor to the growth medium or by altering a physical parameter, e.g. a change in temperature. In the expression vectors used in the preferred embodiments of the present invention, the expression is controlled by the lac repressor. By adding isopropyl-.beta.-D-thiogalactopyranoside (IPTG), the expression control sequence is derepressed and the synthesis of the desired hTNF mutein is thereby induced.

The hTNF muteins of the present invention produced by transformed host cells as stated above can be recovered from the culture medium or after opening the cells and/or extraction by any appropriate method known in protein and peptide chemistry such as, precipitation with ammonium sulfate, dialysis, ultrafiltration, gel filtration or ion-exchange chromatography, gel electrophoresis, isoelectric focusing, affinity chromatography (immunoaffinity chromatography, HPLC or the like). Specifically preferred methods are precipitation with ammonium sulfate and/or polyethylenimine, dialysis, affinity chromatography, e.g. on phenyl-agarose, specifically phenyl-sepharose, or ion-exchange chromatography, specifically on a MONO-Q-and/or MONO-S-matrix (Pharmacia, Uppsala, Sweden) or more specifically are those as described by Tavernier et al. [J. Mol. Biol. 211, 493-501 (1990)] and those disclosed in Example V.

It is therefore also an object of the present invention to provide a process for the preparation of hTNF muteins as specified above which process comprises cultivating a transformed host cell as described above in a suitable medium and isolating a mutein from the culture supernatant or the host cell itself, and if desired converting said mutein into a pharmaceutically acceptable salt. The compounds whenever prepared according to such a process are also an object of the present invention.

The hTNF muteins of the present invention are characterized by showing a selective binding affinity for human p55-TNF-R. Such property can be determined by any assay known in the art for measuring binding affinities. For example the binding of TNF itself and of the muteins of the present invention can be measured using cells in cell culture which express the two types of TNF-receptors to a different degree, for example Hep-2 cells which exclusively express the human p55-TNF-R, and U937 or HL60 cells which express in addition the human p75-TNF-R [see Brockhaus et al., Proc. Nat. Acad. Sci. U.S.A. 87, 3127-3131, (1990); Hohmann et al., J. Biol. Chem. 264, 14927-14934, (1989); Loetscher et al. (1990); Dembic et al. (1990)]. Of course binding affinities can also be determined directly by using purified native or recombinant p55-TNF-R and p75-TNF-R as specifically described in the Examples, or by using the corresponding soluble analogs of such receptors.

The term selective binding affinity for the human p55-Tumor-Necrosis-Factor-Receptor" refers in the context of the present invention to a difference in binding affinities to the two types of TNF-receptors which is with respect to the assay system used significant enough to say that a mutein of the present invention binds selectively to the p55TNF-Receptors similarly to wild-type TNF but has essentially lost functionally relevant binding to hp75-TNF-R. More o specifically this term means in the context of the assay system of the Examples that a K.sub.D value of a specific hTNF mutein of the present invention is at least a factor of 10 or more, especially preferred at least a factor of 10.sup.2, larger than for wild-type TNF-.alpha. determined by using the in vitro binding assay with recombinant soluble hp75-TNF-R whereas its K.sub.D value determined by using the in vitro binding assay to recombinant soluble hp55-TNF-R for the same hTNF mutein differs not by more than a factor of 2 from that of wild-type TNF-.alpha.. It is however understood that these specific K.sub.D values are given for illustration and should not be considered as limiting in any manner. FIGS. 7 and 8 shows that the h-TNF muteins of this invention do not compete with TNF-.alpha. to bind p75-TNF-R, but do competitively bind p55-TNF-R.

The hTNF muteins of the present invention can be characterized by their anti-tumour activity by methods known in the art.

The hTNF muteins may be administered alone or with one or more additional compounds of the present invention in pharmaceutically acceptable oral, injectable or topical compositions and modes. Administration will be in a dosage such that the amount of the composition in the patient is effective to modify the biological function associated with hTNF mutein function.

Pharmaceutical compositions containing hTNF muteins in association with a compatible pharmaceutically acceptable carrier material are therefore a further object of the present invention. Any conventional carrier material can be utilized. The carrier material can be organic or inorganic, suitable for enteral, percutaneous or parenteral administration. Suitable carriers include water, gelatin, gum arabic, lactose, starch, magnesium stearate, talc, vegetable oils, polyalkylene-glycols, petroleum jelly and the like. Furthermore, the pharmaceutical preparations may contain other pharmaceutically active agents. Additional additives such as flavouring agents, preservatives, stabilizers, emulsifying agents, buffers and the like may be added in accordance with accepted practices of pharmaceutical compounding.

The pharmaceutical preparations can be made up in any conventional form including: a) a solid form of oral administration such as tablets, capsules, pills, powders, granules and the like; b) a liquid form for oral administration such as solutions, syrups, suspensions, elixirs and the like; c) preparations for parenteral administration such as sterile solutions, suspensions or emulsions; and d) preparations for topical administrations such as solutions, suspensions, ointments, creams, gels, micronized powders, aerosols and the like. The pharmaceutical preparations may be sterilized and/or may contain adjuvants such as preservatives, stabilizers, wetting agents, emulsifiers, salts for varying the osmotic pressure and/or buffers.

Parenteral dosage forms may be infusions or injectable solutions which can be injected intravenously or intramuscularly. These preparations can also contain other medicinally active substances. Additional additives such as preservatives, stabilizers, emulsifying agents, buffers and the like may be added in accordance with accepted practices of pharmaceutical compounding.

Accordingly it is also an object of the present invention to provide a process for the preparation of a pharmaceutical composition which process is characterized in that a compound obtained by a process of the present invention and if desired, additional pharmaceutically active substances are mixed with a non-toxic, inert, therapeutically compatible carrier material and the mixture is brought into a galenical application form.

Furthermore the use of a compound prepared according to a process of the present invention for the preparation of a pharmaceutical composition as described above is also an object of the present invention.

Finally, antibodies can be raised against the hTNF muteins of the present invention. These antibodies can be used in a well-known manner for diagnostic or therapeutic purposes as well as for purification purposes. Such antibodies can be produced by injecting a mammalian or avian animal with a sufficient amount of a vaccine formulation comprising a hTNF mutein of the present invention and a compatible pharmaceutical carrier to elicit the production of antibodies against said hTNF mutein. The appropriate amount of the hTNF mutein which would be required would be known to one of skill in the art or could be determined by routine experimentation. As used in connection with this invention, the term "pharmaceutical carrier" can mean either the standard compositions which are suitable for human administration or the typical adjuvants employed in animal vaccinations. Monoclonal antibodies can be produced by fusing B-cells of immunized animals with myeloma cells by methods well known in the art, and screening the antibodies produced by the resulting hybridomaa for binding to the h-TFN-.alpha. mutein.

TNF is a potent pleiotropic cytokine. Its many different activities include acting as a growth factor for immune cells, as a mediator in inflammation, or as an inducer of specific genes in endothelium. These activities relate to host defense to infection and injury. TNF also exhibits high systemic toxicity; the deleterious effects of bacteriaemia and septic shock or of bacterial meningitis are mediated to a large extent by endogenous cytokines among which TNF has an early and important role. Furthermore, many cells and cell lines are sensitive to a direct cytotoxic activity of TNF. Various systemic effects and cellular toxicity presumably combine in the antitumor activity of TNF seen in animal studies.

These facts form the rational basis for the development of novel therapeutic strategies using the hTNF muteins of the present invention, where in particular the potential to dissect the many different hTNF activities is exploited to separate unwanted toxic from desired activities. For example, the hTNF muteins of the present inventions may be used as antitumor agents at high doses, which doses are made possible by the lower systemic toxicity. This would overcome the dose-limiting toxicity which presumably severely restricts the use of wild-type hTNF in cancer patients. However, the potential use of the hTNF muteins of the present invention is not restricted to cancer therapy. Any disease where TNF acts as host defense factor in bacterial infection [for example Kindler, V. et al., CELL 56, 731-740 (1989); Nakano, Y. et al., J. Immunol. 144, 1935, (1990)] or as a mediator in inflammation, would be alleviated with a 55kDa TNF receptor type specific drug such as the hTNF muteins of the present invention. TNF has also been shown to play a role in cachexia [e.g. Beutler, B. and Cerami, (sa)] and TNF muteins of the present invention with low systemic toxicity might be used for anti-obesity therapy. Even disease states characterised by the toxic activities exerted by excessive TNF release such as septic shock or bacterial meningitis can benefit from 55kDA TNF receptor specific agonists such as the muteins of the present invention above or in combination with TNF antagonists

p55-TNF-Receptor type specific agonists with lower systemic toxicity than TNF, and selectively triggering only some of the many different TNF activities may be expected to have significant advantages when compared to wild-type TNF. A concise summary of the emerging role of TNF for novel therapies is [Tumor Necrosis Factors, The Molecules and their Emerging Role in Medicine, B. Beutler, ed., Raven Press, 1992, ISBN 0-88167-852-X]. This reference describes the activities of TNF in modulating endothelial cell homeostatic properties and neutrophil adhesion, tissue ischemia and reperfusion injury, on osteoblasts and osteoclasts in bone resorption, as growth factor on many cells in general and in hematopoiesis, as well as in metabolic and nutritional effects. TNF as growth/differentiation factor in the generation of lymphokine-activated killer (LAK) cells appears to contribute to the antitumor activities of TNF. p55-TNF-R controls induction of specific genes providing cellular protection mechanisms such as induction of Mn-superoxide dismutase [Lewis et al, Proc. Natl. Acad. Sci. USA 88, 2830 (1991); Tartaglia et al, Proc. Natl. Acad. Sci. USA 88, 9292 (1991)]. In view of the various important activities of TNF in combination with its direct cytotoxicity in some cells, novel therapeutic strategies using receptor type specific TNF agonists should prove to be important in treating a range of conditions.

An important aspect of this invention is that all these activities may o be enhanced or modulated in combination with other recombinant cytokines such as for example interferon-gamma.

The following Examples are intended to illustrate details of the invention, without thereby limiting it in any manner.

EXAMPLES

Unless otherwise specified, percentages given below for solids in solid mixtures, liquids in liquids and solids in liquids are on a wt/wt, vol/vol and wt/vol basis, respectively.

Example I

Preparation of Thr.sup.86 -TNF.alpha.

Plasmid pDS56/RBSII,SphI-TNF.alpha.

The human TNF.alpha. expression plasmid pDS56/RBSII,SphI-TNF.alpha. (see FIG. 1) was the source of the TNF.alpha. gene for preparation of the various TNF.alpha. muteins of this invention. The transformed E. coli strain M15 [pREP4;pDS56/RBSII,SphI-TNF.alpha.] has been deposited under the Budapest Treaty for patent purposes at the Deutsche Sammlung yon Mikroorganismen und Zellkulturen GmbH (DSM) in Braunschweig, BRD, at Sep. 8th, 1991, under the accession number DSM 6713.

Mutagenesis of the TNF.alpha. gene using PCR

Two PCR reactions were performed with plasmid pDS56/RBSII,SphI-TNF.alpha. (FIG. 1) as the template DNA using a Perkin-Elmer Cetus GeneAmp.sup.TM DNA Amplification Reagent Kit with AmpliTaq.sup.TM Recombinant Taq DNA Polymerase [see FIG. 3].

Reaction I was performed with primers 17/F (5'-GGCGTATCACGAGGCCCTTTCG-3']SEQ ID: 10]; primer 17/F comprises nucleotides 3949-3970 of plasmid pDS56/RBSII,SphI-TNF.alpha.) and 29/M22 (5-GTAGGTGACGGCGATGCGGCTGATGGT-3'[SEQ ID: 15]; primer 29/M22 comprises nucleotides which are complementary to nucleotides 378-352 of plasmid pDS56/RBSII,SphI-TNF.alpha., the mutated base is underlined).

Reaction II was performed with primers 29/MR1 (5'-CAGACCAAGGTCAACCTCCTC-3'[SEQ ID: 16]; primer 29/MR1 comprises nucleotides 379--399 of plasmid pDS56/RBSII,SphI-TNF.alpha.)and 17/O (5'-CATTACTGGATCTATCAACAGG-3'[SEQ ID: 11]; primer 17/O comprises nucleotides which are complementary to nucleotides 748-727 of plasmid pDS56/RBSII,SphI-TNF.alpha.).

In a typical experiment, 10 .mu.l template DNA (10 ng), 5 .mu.l each of the two primers (100 pmoles each), 16 .mu.dNTP's mix (1.25 mM of dATP, dGTP, dCTP, and dTTP), 10 .mu.l 10.times. reaction buffer (100 mM Tris-HCl pH 8.3, 500 mM KCL, 15 mM MgCl.sub.2 and 0.1% gelatin), 1 .mu.l (5 units) AmpliTaq.sup.TM DNA polymerase and 53 .mu.l H.sub.2 O were mixed in an Eppendorf tube and overlaid with 80 ml mineral oil (Perkin-Elmer Cetus). The tubes were transferred to a DNA thermal cycler (TRIO-Thermoblock, Biometra) and kept for 1 min at 94.degree. C., before 35 cycles of melting the DNA (1 min at 94.degree. C.), annealing the primers (1 min at 50.degree. C.), and extending the primers (3 min at 72.degree. C.) were performed. After additional 2 min at 72.degree. C., the reactions were cooled to room temperature and extracted with chloroform. The DNA present in the aqueous phase was precipitated with ethanol and subjected to electrophoresis in a 6% polyacrylamide gel [Sambrook et al., 1989]. After staining of the DNA with ethidium bromide, fragments I and II (see FIG. 3) were isolated from the gel and purified [Sambrook et al., 1989].

Preparation of a DNA fragment encoding Thr.sup.86 -TNF.alpha.

Fragments I and II were enzymatically phosphorylated, before they were ligated with each other [Sambrook et al., 1989]. After heat-inactivation of the ligase and digestion with restriction enzymes EcoRI and HindIII, the DNA was subjected to electrophoresis in a 6% polyacrylamide gel. After staining of the DNA with ethidium bromide, the EcoRI-HindIII fragment A [see FIG. 3] was isolated from the gel and purified [s.a].

Preparation of a plasmid encoding Thr.sup.86 -TNF.alpha.

The EcoRI-HindIII fragment A was inserted according to standard methods [Sambrook et al., 1989] into the EcoRI-HindIII opened plasmid pDS56/RBSII,SphI-TNF.alpha. generating the plasmid pDS56/RBSII,SphI-TNF.alpha.(Thr86). Plasmid DNA was prepared [Birnboim et al., 1979] and the identity of the coding region for the TNF.alpha. mutein was confirmed by sequencing the double-stranded DNA [Sambrook et al., 1989].

Production of Thr.sup.86 -TNF.alpha.

Plasmid pDS56/RBSII,SphI-TNF.alpha.(Thr86) was transformed into E. coli M15 cells containing already plasmid pREP4 by standard methods [s.a.]. Transformed cells were grown at 37.degree. C. in LB medium [Sambrook et al., 1989] containing 100 mg/1 ampicillin and 25 mg/l kanamycin. At an optical density at 600 nm of about 0.7 to 1.0 IPTG was added to a final concentration of 2 mM. After additional 2.5 to 5 h at 37.degree. C. the cells were harvested by centrifugation.

Example II

Preparation of Glu.sup.31 -TNF.alpha.and Asn.sup.31 Thr.sup.32 TNF.alpha.

Principles

The TNF.alpha. muteins Glu.sup.31 -TNF.alpha. and Asn.sup.31 Thr.sup.32 TNF.alpha. were prepared following the procedure described in detail in Example I for the preparation of Thr.sup.86 -TNF.alpha.. Therefore, in the description of the preparation of the TNF.alpha. muteins listed above only the primers used in PCR reactions I and II are specified. Furthermore, the names of the expression plasmids encoding the various TNF.alpha. muteins are given.

Preparation of Glu.sup.31 -TNF.alpha.

PCR reaction I was performed with primers 17/F (5'-GGCGTATCACGAGGCCCTTTCG-3'[SEQ ID: 10]; primer 17/F comprises nucleotides 3949-3970 of plasmid pDS56/RBSII,SphI-TNF.alpha.) and 21/M5 (5-ATTGGCCCGCTCGTTCAGCCACTGGAGCTGCCCCTC-3'[SEQ ID: 12]; primer 21/M5 comprises nucleotides which are complementary to nucleotides 219-184 of plasmid pDS56/RBSII,SphI-TNF.alpha., mutated bases are underlined). PCR reaction II was performed with primers 21/MR (5'-GCCCTCCTGGCCAATGGCGTGG-3'[SEQ ID: 14]; primer 21/MR comprises nucleotides 220-241 of plasmid pDS56/RBSII,SphI-TNF.alpha.) and 17/O (5'-CATTACTGGATCTATCAACAGG-3'[SEQ ID: 11]; primer 17/O comprises nucleotides which are complementary to nucleotides 748-727 of plasmid pDS56/RBSII,SphI-TNF.alpha.).

The resulting expression plasmid pDS56/RBSII,SphI-TNF.alpha.(Glu31) was used for production of Glu.sup.31 -TNF.alpha. and in the construction of plasmid pDS56/RBSII,SphI-TNF.alpha.(GIu31Thr86) (see Example IV).

Preparation of Asn.sup.31 Thr.sup.32 -TNF.alpha.

PCR reaction I was performed with primers 17/F (5'-GGCGTATCACGAGGCCCTTTCG-3'[SEQ ID: 10]; primer 17/F comprises nucleotides 3949-3970 of plasmid pDS56/RBSII,SphI-TNF.alpha.) and 21/M6 (5-ATTGGCAGTGTTGTTCAGCCACTGGAGCTGCCCCTC-3'[SEQ ID: 13]; primer 21/M6 comprises nucleotides which are complementary to nucleotides 219-184 of plasmid pDS56/RBSII,SphI-TNF.alpha., mutated bases are underlined). PCR reaction II was performed with primers 21/MR (5'-GCCCTCCTGGCCAATGGCGTGG-3'[SEQ ID: 14]; primer 21/MR comprises nucleotides 220-241 of plasmid pDS56/RBSII,SphI-TNF.alpha.) and 17/O (5'-CATTACTGGATCTATCAACAGG-3'[SEQ ID: 11]; primer 17/O comprises nucleotides which are complementary to nucleotides 748-727 of plasmid pDS56/RBSII,SphI-TNF.alpha.).

The resulting expression plasmid pDS56/RBSII,SphI-TNF.alpha.(Asn31Thr32) was used for production of Asn.sup.31 Thr.sup.32 -TNF.alpha. and in the construction of plasmid pDS56/RBSII,SphI-TNF.alpha.(Asn31Thr32Thr86) (see Example IV).

Example III

Preparation of Trp.sup.32 Thr.sup.86 -TNF.alpha.

Principles

For preparation of Trp.sup.32 Thr.sup.86 -TNF.alpha. the expression plasmid pDS56/RBSII,SphI-TNF.alpha.(Trp32Thr86) was constructed, which was subsequently used for the production of Trp.sup.32 Thr.sup.86 -TNF.alpha. in E. coli. Construction of plasmid pDS56/RBSII,SphI-TNF.alpha.(Trp32Thr86)

All the expression plasmids described in Examples I and II contain the same two sites for the restriction enzyme BglI as plasmid pDS56/RBSII,SphI-TNF.alpha. (see FIG. 1). One of these sites is located in the .beta.-lactamase gene whereas the other site is located in the TNF.alpha. gene. This latter site separates the coding region for TNF.alpha. into two parts: one part is coding for amino acids 1 to 36 of TNF.alpha., the other part encodes amino acids 37 to 157 of TNF.alpha. (see FIG. 1b1).

For construction of plasmid pDS56/RBSII,SphI-TNF.alpha.(Trp32Thr86) DNA fragments 1 and 2 were prepared according to standard methods [Sambrook et al., 1989]. Fragment 1 (for sequences see FIG. 4) was the small BglI fragment of plasmid pDS56/RBSII,SphI-TNF.alpha.(Trp32) with the regulatable promoter and the coding region for Trp32-TNF.alpha. up to amino acid 36. Fragment 2 was the large BglI fragment of plasmid pDS56/RBSII,SphI-TNF.alpha.(Thr86) with the coding region for Thr.sup.86 -TNF.alpha. starting at amino acid 37 and the replication region of the plasmid. Fragment 1 and the enzymatically dephosphorylated fragment 2 were ligated with each other [Sambrook et al., 1989] resulting in plasmid pDS56/RBSII,SphI-TNF.alpha.(Trp32Thr86).

M15(pREP4;pDS56/RBSII,SphI-TNF.alpha.(Trp32)) cells have been deposited under the Budapest Treaty for patent purposes at the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM) in Braunschweig, BRD at Nov. 19th, 1990 under accession number DSM 6241.

Production of Trp.sup.32 Thr.sup.86 -TNF.alpha.

Plasmid pDS56/RBSII,SphI-TNF.alpha.(Trp32Thr86) was transformed into E. coli M15 cells containing already plasmid pREP4 by standard methods [s.a.]. Transformed cells were grown at 37.degree. C. in LB medium [s.a.] containing 100 mg/l ampicillin and 25 mg/1 kanamycin. At an optical density at 600 nm of about 0.7 to 1.0 IPTG was added to a final concentration of 2 mM. After additional 2.5 to 5 h at 37.degree. C. the cells were harvested by centrifugation.

Example IV.

Preparation of Ser.sup.29 Thr.sup.86 -TNF.alpha., Ser.sup.29 Trp.sup.32 Thr.sup.86 -TNF.alpha., Glu.sup.31 Thr.sup.86 -TNF.alpha. and Asn.sup.31 Thr.sup.32 Thr.sup.86 TNF.alpha.

Principles

The TNF.alpha. muteins Ser.sup.29 Thr.sup.86 -TNF.alpha., Ser.sup.29 Trp.sup.32 Thr.sup.86 -TNF.alpha., Glu.sup.31 Thr.sup.86 -TNF.alpha. and Asn.sup.31 Thr.sup.32 Thr.sup.86 -TNF.alpha. were prepared following the procedure described in detail in Example III for the preparation of Trp.sup.32 Thr.sup.86 -TNF.alpha.. Therefore, in the description of the preparation of the TNF.alpha. muteins listed above only the DNA fragments corresponding to fragment 1 of Example III are specified. Furthermore, the names of the expression plasmids encoding the various TNF.alpha. muteins are given.

Preparation of Ser.sup.29 Thr.sup.86 -TNF.alpha.

Fragment 1 (for sequences see FIG. 5) was the small BglI fragment of plasmid pDS56/RBSII,SphI-TNF.alpha.(Ser29) with the regulatable promoter and the coding region for Ser.sup.29 -TNF.alpha. up to amino acid 36. Fragment 1 and the enzymatically dephosphorylated fragment 2 (see Example III) were ligated with each other [Sambrook et al., 1989] resulting in plasmid pDS56/RBSII,SphI-TNF.alpha.(Ser29Thr86), which was subsequently used for the production of Ser.sup.29 Thr.sup.86 -TNF.alpha. in E. coli.

M15 (pREP4;pDS56/RBSII,SphI-TNF.alpha.(Ser29)) cells have been deposited under the Budapest Treaty for patent purposes at the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM) in Braunschweig, BRD at Nov. 19th, 1990 under accession number DSM 6240.

Preparation of Ser.sup.29 Trp.sup.32 Thr.sup.86 -TNF.alpha.

Fragment 1 (for sequences see FIG. 6) was the small BglI fragment of plasmid pDS56/RBSII,SphI-TNF.alpha.(Ser29Trp32) with the regulatable promoter and the coding region for Ser.sup.29 Trp.sup.32 -TNF.alpha. up to amino acid 36. Fragment 1 and the enzymatically dephosphorylated fragment 2 (see Example III) were ligated with each other [Sambrook et al., 1989] resulting in plasmid pDS56/RBSII,SphI-TNF.alpha.(Ser29Trp32Thr86), which was subsequently used for the production of Ser.sup.29 Trp.sup.32 Thr.sup.86 -TNF.alpha. in E. coli.

Preparation of Glu.sup.31 Thr.sup.86 TNF.alpha.

Fragment 1 was the small Bgll fragment of plasmid pDS56/RBSII,SphI-TNF.alpha.(Glu31) with the regulatable promoter and the coding region for Glu.sup.31 -TNF.alpha. up to amino acid 36. Fragment 1 and the enzymatically dephosphorylated fragment 2 (see Example III) were ligated with each other [Sambrook et al., 1989] resulting in plasmid pDS56/RBSII,SphI-TNF.alpha.(Glu31Thr86), which was subsequently used for the production of Glu.sup.31 Thr.sup.86 -TNF.alpha. in E. coli.

Preparation of Asn.sup.31 Thr.sup.32 Thr.sup.86 TNF.alpha.

Fragment 1 was the small BglI fragment of plasmid pDS56/RBSII,SphI-TNF.alpha.(Asn31Thr32) with the regularable promoter and the coding region for Asn.sup.31 Thr.sup.32 -TNF.alpha. up to amino acid 36. Fragment 1 and the enzymatically dephosphorylated fragment 2 (see Example III) were ligated with each other [Sambrook et al., 1989] resulting in plasmid pDS56/RBSII,SphI-TNF.alpha.(Asn31Thr32Thr86), which was subsequently used for the production of Asn31Thr32Thr86-TNF.alpha. in E. coli.

Example V

Purification of Human TNF.alpha. Muteins

One liter overnight cultures of E. coli cells transformed and induced as described above were collected by centrifugation and resuspended in 20 ml 50 mM Tris, pH 7.2, 200 mM KCl, 50 mM MgCl.sub.2, 5% glycerol. The cells were disrupted in a French press at a pressure of 20,000 psi. After clarification by centrifugation (70'000.times.g, 30 min, 4.degree. C.) solid ammonium sulfate was added to a final concentration of 30%. The solution was stirred at room temperature for one hour and then centrifuged at 10'000.times.g for 20 min at 4.degree. C. The supernatant was filtered through a 0.45 .mu.m filter and adjusted to 70% in ammonium sulfate. The precipitated proteins were collected by centrifugation, dissolved in 20 ml 20 mM Tris, pH 9.0, and dialyzed against the same buffer overnight at 4.degree. C. 1 ml aliquots of the dialyzed samples were applied to a MonoQ column (HR 5/5, LKB-Pharmacia) equilibrated in 20 mM Tris pH 9.0 and eluted with a linear NaCl gradient (0 to 400 mM in 20 mM Tris pH 9.0) at a flow rate of 0.5 ml/min. 0.5 ml fractions were collected and analyzed for the presence of TNF.alpha. muteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Positive fractions were pooled, dialyzed against 20 mM 2-morpholinoethanesulfonic acid (MES) pH 6.0 and applied to a MonoS column (HR 5/5, LKB-Pharmacia) equilibrated in 20 mM MES pH 6.0. Proteins were eluted with a linear NaCl gradient (0 to 400 mM in 20 mM MES pH 6.0) at a flow rate of 0.5 ml/min. The various TNF.alpha. muteins eluted as electrophoretically pure proteins between 250 mM and 350 mM NaCl. After dialysis against phosphate buffered saline (PBS) the protein concentration was determined by the BCA Protein Assay (Pierce Chemical Company) using wild-type human TNF.alpha. as a standard.

Example VI

Competitive Binding of Human TNF.alpha. and Muteins to Recombinant Human p75-TNF-R and p55-TNF-R

For the competitive binding assay microtiter plates were coated with recombinant human p75-TNF-R-human IgG.gamma.3 and p55-TNF-R-human IgG.gamma.3 fusion proteins dissolved in PBS at 0.3 .mu.g/ml and 0.1 .mu.g/ml, respectively, (100 .mu.l/well, overnight at 4.degree. C.) [Loetscher, H. et al., J. Biol. Chem. 266, 18324-18329 (1991); Lesslauer, W. et al., Eur. J. Immunol. 21, 2883-2886 (1991)]. After blocking with blocking buffer (50 mM Tris pH 7.4, 140 mM NaCl, 5 mM EDTA, 0.02% NAN.sub.3, 1% defatted milk powder) the microtiler plate was washed with PBS and incubated with 10 ng/ml human .sup.125 I-TNF.alpha. (labelled by the Iodogen method (Pierce Chemical Company) to a specific activity of about 30 .mu.Ci/.mu.g) in the presence of different concentrations of the muteins. The volume was 100 .mu.l/well and each concentration was assayed in triplicate. After three hours at room temperature the wells were thoroughly washed with PBS and counted in a g-counter.

__________________________________________________________________________ SEQUENCE LISTING (1) GENERAL INFORMATION: (iii) NUMBER OF SEQUENCES: 17 (2) INFORMATION FOR SEQ ID NO: 1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3977 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (v) FRAGMENT TYPE: internal (vi) ORIGINAL SOURCE: (A) ORGANISM: Plasmid pDS56/RBSII,SphI-TNFalpha (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 118..591 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: CTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCA 60 ATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAAGCATG117 GTCAGATCATCTTCTCGAACCCCGAGTGACAAGCCTGTAGCCCATGTT165 ValArgSerSerSerArgThrProSerAspLy sProValAlaHisVal 151015 GTCGCGAACCCTCAAGCTGAGGGGCAGCTCCAGTGGCTGAACCGCCGG213 ValAlaAsnProGlnAlaGluGlyGlnLe uGlnTrpLeuAsnArgArg 202530 GCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGAGATAACCAGCTG261 AlaAsnAlaLeuLeuAlaAsnGlyValGl uLeuArgAspAsnGlnLeu 354045 GTGGTGCCATCAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTC309 ValValProSerGluGlyLeuTyrLeuIleTy rSerGlnValLeuPhe 505560 AAGGGCCAAGGCTGCCCCTCCACCCATGTGCTCCTCACCCACACCATC357 LysGlyGlnGlyCysProSerThrHisValLeuLeuTh rHisThrIle 65707580 AGCCGCATCGCCGTCTCCTACCAGACCAAGGTCAACCTCCTCTCTGCC405 SerArgIleAlaValSerTyrGlnThrLysVa lAsnLeuLeuSerAla 859095 ATCAAGAGCCCCTGCCAGAGGGAGACCCCAGAGGGGGCTGAGGCCAAG453 IleLysSerProCysGlnArgGluThrPr oGluGlyAlaGluAlaLys 100105110 CCCTGGTATGAGCCCATCTATCTGGGAGGGGTCTTCCAGCTGGAGAAG501 ProTrpTyrGluProIleTyrLeuGlyGl yValPheGlnLeuGluLys 115120125 GGTGACCGACTCAGCGCTGAGATCAATCGGCCCGACTATCTCGACTTT549 GlyAspArgLeuSerAlaGluIleAsnArgPr oAspTyrLeuAspPhe 130135140 GCCGAGTCTGGGCAGGTCTACTTTGGGATCATTGCCCTGTGAGGAGGAC598 AlaGluSerGlyGlnValTyrPheGlyIleIleAlaLe u 145150155 GAACATCCAACCTTCCCAAACGCCTCCCCTGCCCCAATCCCTTTATTACCCCCTCCTTCA658 GACACCCTCAACCTCTTCTGGCTCAAAAAGAGAATTGGGGGCTTAGGGTCGGAACCCAAG718 CTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGA778 ACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATT838 TTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTG GATATACCACCGTTGATAT898 ATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTA958 TAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCA1018 CAAGTTTTATCCGGCC TTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATT1078 TCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACAC1138 CGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTT 1198 CCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTA1258 TTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTT1318 CACCAGTTTTGATTTAAACGTGGCCAATATGGAC AACTTCTTCGCCCCCGTTTTCACCAT1378 GGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCA1438 TGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGA1498 TGAGTGGCA GGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGG1558 GGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGG1618 CCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAA TCCGCCGC1678 TCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCC1738 CGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCG1798 CGTCAGCGGGTGTTGGCGGGTGTCGGG GCGCAGCCATGACCCAGTCACGTAGCGATAGCG1858 GAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATAT1918 GCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGC1978 T TCCTCGCTCACTGACTCGCTGCGCTCGGTCTGTCGGCTGCGGCGAGCGGTATCAGCTCA2038 CTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTG2098 AGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT GCTGGCGTTTTTCCA2158 TAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAA2218 CCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCC2278 TGTTCCGACCCTGCCGCTTA CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGC2338 GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCT2398 GGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCG2 458 TCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAG2518 GATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA2578 CGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC TGCTGAAGCCAGTTACCTTCGG2638 AAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTT2698 TGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTT2758 TTCTACGGGGTC TGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAG2818 ATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAAT2878 CTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGG CACC2938 TATCTCAGCGATCTGTCTATTTCGTTCATCCATAGCTGCCTGACTCCCCGTCGTGTAGAT2998 AACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCC3058 ACGCTCACCGGCTCCAGATTTATCAGCAAT AAACCAGCCAGCCGGAAGGGCCGAGCGCAG3118 AAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAG3178 AGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGT3238 GGTGT CACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCG3298 AGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGT3358 TGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCA CTGCATAATTC3418 TCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTC3478 ATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAA3538 TACCGCGCCACATAGCAGAACTT TAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCG3598 AAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACC3658 CAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAG3718 GCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTT3778 CCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATT3838 TGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCA CATTTCCCCGAAAAGTGCC3898 ACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCAC3958 GAGGCCCTTTCGTCTTCAC3977 (2) INFORMATION FOR SEQ ID NO: 2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 157 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: ValArgSerSerSerArgThrProSerAspLysProValAlaHisVal 15 1015 ValAlaAsnProGlnAlaGluGlyGlnLeuGlnTrpLeuAsnArgArg 202530 AlaAsnAlaLeuLeuAlaAsnGlyValGluLeuA rgAspAsnGlnLeu 354045 ValValProSerGluGlyLeuTyrLeuIleTyrSerGlnValLeuPhe 505560 LysGly GlnGlyCysProSerThrHisValLeuLeuThrHisThrIle 65707580 SerArgIleAlaValSerTyrGlnThrLysValAsnLeuLeuSerAla 859095 IleLysSerProCysGlnArgGluThrProGluGlyAlaGluAlaLys 100105110 ProTrpTyrGluPro IleTyrLeuGlyGlyValPheGlnLeuGluLys 115120125 GlyAspArgLeuSerAlaGluIleAsnArgProAspTyrLeuAspPhe 130135 140 AlaGluSerGlyGlnValTyrPheGlyIleIleAlaLeu 145150155 (2) INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3740 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Plasmid pREP4 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3: AAGCTTCACGCTGCCGCAAGCACTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGT60 AGAAAGC CAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCT120 GGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGC180 GATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCA GCTGGGGCGC240 CCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGA300 TCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGC360 TTGAACAAGATGGATTGCACGCAGG TTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCT420 ATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGC480 AGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGG540 ACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCG600 ACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATC660 TCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCAT GGCTGATGCAATGCGGC720 GGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCG780 AGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGC840 ATCAGGGGCTCGCGCCAG CCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCG900 AGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCC960 GCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAG 1020 CGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCG1080 TGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACG1140 AGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGAC CGACCAAGCGACGCCCAACCTGCC1200 ATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTT1260 CCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCA1320 CCCCGGGCTC GATCCCCTCGCGAGTTGGTTCAGCTGCTGCCTGAGGCTGGACGACCTCGC1380 GGAGTTCTACCGGCAGTGCAAATCCGTCGGCATCCAGGAAACCAGCAGCGGCTATCCGCG1440 CATCCATGCCCCCGAACTGCAGGAGTGGGGAGGCACGATGGCCGCTTTGGTCGA CAATTC1500 GCGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTC1560 GTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG1620 CCAGGGTGGTTTTTCTTTTCACCAGTGAG ACGGGCAACAGCTGATTGCCCTTCACCGCCT1680 GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT1740 GTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCA1800 CTA CCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCA1860 GCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTT1920 GCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGT TCCGCTATCGGCT1980 GAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAG2040 AACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCA2100 CGCCCAGTCGCGTACCGTCTT CATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAG2160 AGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCT2220 GGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCA228 0 CCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCAC2340 CCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCA2400 GACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCC CGCCAGTTGTTGTGCCACGC2460 GGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAG2520 AAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACT2580 CTGCGACATCGTAT AACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCG2640 GGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGC2700 ATGCCGCTTCGCCTTCGCGCGCGAATTGTCGACCCTGTCCCTCCTGTTCAGCTACTGA CG2760 GGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTAGCGGAGTGTATACTGGC2820 TTACTATGTTGGCACTGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAG2880 GCTGCACCGGTGCGTCAGCAGAATATGTGATA CAGGATATATTCCGCTTCCTCGCTCACT2940 GACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGAAATGGCTTACGAACGGGGCGGA3000 GATTTCCTGGAAGATGCCAGGAAGATACTTAACAGGGAAGTGAGAGGGCCGCGGCAAAGC3060 CGTTTTT CCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT3120 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCTGGCGGCTCCCTCGT3180 GCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCATTCCGCTGTTAT GGCCGCGTTT3240 GTCTCATTCCACGCCTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTGGACTGTA3300 TGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT3360 CCAACCCGGAAAGACATGCAAAAGC ACCACTGGCAGCAGCCACTGGTAATTGATTTAGAG3420 GAGTTAGTCTTGAAGTCATGCGCCGGTTAAGGCTAAACTGAAAGGACAAGTTTTGGTGAC3480 TGCGCTCCTCCAAGCCAGTTACCTCGGTTCAAAGAGTTGGTAGCTCAGAGAACCTTCGAA3540 AAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGATTACGCGCAGACCAAAA3600 CGATCTCAAGAAGATCATCTTATTAATCAGATAAAATATTTCTAGATTTCAGTGCAATTT3660 ATCTCTTCAAATGTAGCACCTGAAGTCAGCCCCATACGATATA AGTTGTTAATTCTCATG3720 TTTGACAGCTTATCATCGAT3740 (2) INFORMATION FOR SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1106 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ( D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (v) FRAGMENT TYPE: N-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Plasmid pDS56/RBSII,SphI-THFalpha(Trp32) (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 994..1104 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4: GGAAGGGCCGAGCGCAGAAGTGGTCCTGCA ACTTTATCCGCCTCCATCCAGTCTATTAAT60 TGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCC120 ATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGT180 TCCCA ACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCC240 TTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATG300 GCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTT CTGTGACTGGT360 GAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCG420 GCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGA480 AAACGTTCTTCGGGGCGAAAACT CTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATG540 TAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGG600 TGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGT660 TGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTC720 ATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACA780 TTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTAT TATCATGACATTAACCTAT840 AAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCACCTCGAGAAATCATAAAAAATTTAT900 TTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCAC960 ACAGAATTCATTAAAG AGGAGAAATTAAGCATGGTCAGATCATCTTCTCGAACC1014 ValArgSerSerSerArgThr 15 CCGAGTGACAAG CCTGTAGCCCATGTTGTAGCAAACCCTCAAGCTGAG1062 ProSerAspLysProValAlaHisValValAlaAsnProGlnAlaGlu 101520 GGGCAGCTCCAGTGG CTGAACCGCTGGGCCAATGCCCTCCTG1104 GlyGlnLeuGlnTrpLeuAsnArgTrpAlaAsnAlaLeuLeu

253035 GC 1106 (2) INFORMATION FOR SEQ ID NO: 5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 37 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5: ValArgSerSerSerArgThrProSerAspL ysProValAlaHisVal 151015 ValAlaAsnProGlnAlaGluGlyGlnLeuGlnTrpLeuAsnArgTrp 2025 30 AlaAsnAlaLeuLeu 35 (2) INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1106 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (v) FRAGMENT TYPE: N-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Plasmid pDS56/RBSII,SphI-TNFalpha(Ser29) (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 994..1104 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6: GGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAAT60 TGTTGCCGGGA AGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCC120 ATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGT180 TCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTA GCTCC240 TTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATG300 GCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGT360 GAGTACTCAACCAAGTCATTCTGAGAATAG TGTATGCGGCGACCGAGTTGCTCTTGCCCG420 GCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGA480 AAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATG540 TAAC CCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGG600 TGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGT660 TGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAG GGTTATTGTCTC720 ATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACA780 TTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTAT840 AAAAATAGGCGTATCACGAGGC CCTTTCGTCTTCACCTCGAGAAATCATAAAAAATTTAT900 TTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCAC960 ACAGAATTCATTAAAGAGGAGAAATTAAGCATGGTCAGATCATCTTCTCGAACC1014 ValArgSerSerSerArgThr 15 CCGAGTGACAAGCCTGTAGCCCATGTTGTAGCAAACCCTCAAGCTGAG10 62 ProSerAspLysProValAlaHisValValAlaAsnProGlnAlaGlu 101520 GGGCAGCTCCAGTGGTCCAACCGCCGGGCCAATGCCCTCCTG1104 GlyGlnLeuGlnTrpSerAsnArgArgAlaAsnAlaLeuLeu 253035 GC1106 (2) INFORMATION FOR SEQ ID NO: 7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 37 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7: ValArgSerSerSerArgThrProSerAspLysProValAlaHisVal 15 1015 ValAlaAsnProGlnAlaGluGlyGlnLeuGlnTrpSerAsnArgArg 202530 AlaAsnAlaLeuLeu 35 (2) INFORMATION FOR SEQ ID NO: 8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1106 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (v) FRAGMENT TYPE: N-terminal (vi) ORIGINAL SOURCE: (A) ORGANISM: Plasmid pDS56/RBSII,SphI-TNFalpha(Ser29Trp32) (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 994..1104 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8: GGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAAT60 TGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCC 120 ATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGT180 TCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCC240 TTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCC GCAGTGTTATCACTCATGGTTATG300 GCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGT360 GAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCG420 GCGTCAATAC GGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGA480 AAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATG540 TAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTT TCTGGG600 TGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGT660 TGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTC720 ATGAGCGGATACATATTTGAATGTATTTA GAAAAATAAACAAATAGGGGTTCCGCGCACA780 TTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTAT840 AAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCACCTCGAGAAATCATAAAAAATTTAT900 TTG CTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCAC960 ACAGAATTCATTAAAGAGGAGAAATTAAGCATGGTCAGATCATCTTCTCGAACC1014 ValArgSerSer SerArgThr 15 CCGAGTGACAAGCCTGTAGCCCATGTTGTAGCAAACCCTCAAGCTGAG1062 ProSerAspLysProValAlaHisValValAlaAsnPr oGlnAlaGlu 101520 GGGCAGCTCCAGTGGTCCAACCGCTGGGCCAATGCCCTCCTG1104 GlyGlnLeuGlnTrpSerAsnArgTrpAlaAsnAlaLeuLe u 253035 GC1106 (2) INFORMATION FOR SEQ ID NO: 9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 37 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9: ValArgSerSerSerArgThrProSerAspLysProValAlaHisVal 151015 ValAlaAsnProGlnAl aGluGlyGlnLeuGlnTrpSerAsnArgTrp 202530 AlaAsnAlaLeuLeu 35 (2) INFORMATION FOR SEQ ID NO: 10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Primer 17/F (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: GGCGTATCACGAGGCCCTTTCG 22 (2) INFORMATION FOR SEQ ID NO: 11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Primer 17/0 (x i) SEQUENCE DESCRIPTION: SEQ ID NO: 11: CATTACTGGATCTATCAACAGG22 (2) INFORMATION FOR SEQ ID NO: 12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 36 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Primer 21/M5 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12: ATTGGCCCGCTCGTTCAGCCACTGGAGCTGCCCCTC36 (2) INFORMATION FOR SEQ ID NO: 13: (i) SEQUENCE CHARACTERISTICS: ( A) LENGTH: 36 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Primer 21/M6 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13: ATTGGCAGTGTTGTTCAGCCACTGGAGCTGCCCCTC 36 (2) INFORMATION FOR SEQ ID NO: 14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Primer 21/MR (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14: GCCCTCCTGGCCAATGGCGTGG22 (2) INFORMATION FOR SEQ ID NO: 15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Primer 29/M22 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15: GTAGGTGACGGCGATGCGGCTGATGGT27 (2) INFORMATION FOR SEQ ID NO: 16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Primer 29/MR1 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16: CAGACCAAGGTCAACCT CCTC21 (2) INFORMATION FOR SEQ ID NO: 17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 157 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17: ValArgSerSerSerArg ThrProSerAspLysProValAlaHisVal 151015 ValAlaAsnProGlnAlaGluGlyGlnLeuGlnTrpXaaAsnXaaXaa 20 2530 AlaAsnAlaLeuLeuAlaAsnGlyValGluLeuArgAspAsnGlnLeu 354045 ValValProSerGluGlyLeuTyrLeuIleTyrSerG lnValLeuPhe 505560 LysGlyGlnGlyCysProSerThrHisValLeuLeuThrHisThrIle 65707580 SerArgIleAlaValSerTyrGlnThrLysValAsnLeuLeuSerAla 859095 IleLysSerProCysGlnArgGluThrProGluGlyAlaGluAlaLys 100105110 ProTrpTyrGluProIleTyrLeuGlyGlyValPheGlnLeuGluLys 115120125 GlyAspArgLeuSerAla GluIleAsnArgProAspTyrLeuAspPhe 130135140 AlaGluSerGlyGlnValTyrPheGlyIleIleAlaLeu 145150155