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Bioaccessibility and antioxidant activity of free phenolic compounds and oligosaccharides from corn (Zea mays L.) and common bean (Phaseolus vulgaris L.) chips during in vitro gastrointestinal digestion and simulated colonic fermentation.

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Corn (Zea mays L.) and common beans (Phaseolus vulgaris L.) are alternative suitable ingredients for snacks, because of their content of bioactive compounds such as phenolic compounds (PC) and oligosaccharides (OS). However, there is no information about the transformation of these compounds

The effects of different high-fat (lard, soybean oil, corn oil or olive oil) diets supplemented with fructo-oligosaccharides on colonic alkaline phosphatase activity in rats.

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We recently reported that fermentable non-digestible carbohydrates including fructo-oligosaccharides (FOS) commonly elevate colonic alkaline phosphatase (ALP) activity and the expression of IAP-I, an ALP gene, in rats fed a high-fat (HF) diet, and also elevate gut mucins and modulate

Practice and mechanism of HPLC oligosaccharide separation with a cyclodextrin bonded phase.

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A series of six oligosaccharides: linear arabinosides, cellosides, isomaltosides, mannosides, maltosides and xylosides containing up to 15 sugar units, as well as alpha, beta and gamma-cyclodextrins, were separated without derivatization with acetonitrile-water mobile phases and a beta-cyclodextrin

Gtgen3A, a novel plant GH3 β-glucosidase, modulates gentio-oligosaccharide metabolism in Gentiana.

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Gentiobiose, a β-1,6-linked glycosyl-disaccharide, accumulates abundantly in Gentianaceae and is involved in aspects of plant development, such as fruits ripening and release of bud dormancy. However, the mechanisms regulating the amount of gentio-oligosaccharide accumulation in plants remain

Inositol Metabolism in Plants. V. Conversion of Myo-inositol to Uronic Acid and Pentose Units of Acidic Polysaccharides in Root-tips of Zea mays.

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The metabolism of myo-inositol-2-(14)C, d-glucuronate-1-(14)C, d-glucuronate-6-(14)C, and l-methionine-methyl-(14)C to cell wall polysaccharides was investigated in excised root-tips of 3 day old Zea mays seedlings. From myo-inositol, about one-half of incorporated label was recovered in ethanol

Enzymic Analysis of Feruloylated Arabinoxylans (Feraxan) Derived from Zea mays Cell Walls : II. Fractionation and Partial Characterization of Feraxan Fragments Dissociated by a Bacillus subtilis Enzyme (Feraxanase).

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Structural features of feruloylated arabinoxylan (feraxan) present in Zea mays L. (hybrid B 73 x Mo 17) coleoptile cell walls have been studied using a purified feraxan-dissociating enzyme (feraxanase) and an alpha-arabinofuranosidase. This experimental approach has demonstrated the following. (a)

Separation of dansyl hydrazine-derivatized oligosaccharides by liquid chromatography.

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A sensitive method has been developed for the rapid separation of neutral oligosaccharides derivatized with dansyl hydrazine by liquid chromatography on silica gel modified with 1,4-diaminobutane. Malto-oligosaccharides from corn syrup in amounts ranging from 7 to 280 nmol and ovalbumin-derived

Hydrolytic Activity and Substrate Specificity of an Endoglucanase from Zea mays Seedling Cell Walls.

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An endoglucanase was isolated from cell walls of Zea mays seedlings. Characterization of the hydrolytic activity of this glucanase using model substrates indicated a high specificity for molecules containing intramolecular (1-->3),(1-->4)-beta-d-glucosyl sequences. Substrates with

Development of a novel fluorescent tag O-2-[aminoethyl]fluorescein for the electrophoretic separation of oligosaccharides.

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This study describes the development of a novel fluorescent tag, O-2-[aminoethyl]fluorescein, for the separation of sugars by capillary electrophoresis with fluorescence detection using an argon ion laser. The tag was synthesised using three consecutive steps namely: esterification, alkylation and

Impact of galactoglucomannan oligosaccharides and Cd stress on maize root growth parameters, morphology, and structure.

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Biologically active oligosaccharides, including galactoglucomannan oligosaccharides (GGMOs), affect plant growth and development. The impact of GGMOs is dependent on their concentration, and the plant species and plant parts affected. The aim of this article is to ascertain the effects of GGMOs,

Safety of xylo-oligosaccharides (XOS) as a novel food pursuant to Regulation (EU) 2015/2283

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Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on a mixture of xylo-oligosaccharides (XOS) as a novel food (NF) pursuant to Regulation (EU) 2015/2283. The NF is obtained from corncobs

High-Throughput Monitoring of Multiclass Syrup Adulterants in Honey Based on the Oligosaccharide and Polysaccharide Profiles by MALDI Mass Spectrometry.

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Honey is a natural product that could be easily adulterated with various cheaper sweeteners. In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles.

Cross-linked α-galactosidase aggregates: optimization, characterization and application in the hydrolysis of raffinose-type oligosaccharides in soymilk.

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Cross-linked enzyme aggregates (CLEAs) of α-galactosidase, partially purified from maize (Zea mays) flour, were prepared. The impact of various parameters on enzyme activity was examined to optimize the immobilization procedure. Biochemical characterization of the free and immobilized

Batch and fed-batch carotenoid production by Rhodotorula glutinis-Debaryomyces castellii co-cultures in corn syrup.

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OBJECTIVE Investigations on the production of red pigments by Rhodotorula glutinis on raw substrates of agro-industrial origin may be considered of interest because they represent the first approach to the utilization of these raw materials for biotechnological purposes. RESULTS Rhodotorula glutinis

Analysis of Popcorn (Zea mays L. var. everta) for Antioxidant Capacity and Total Phenolic Content.

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Popcorn, one of the most popular snack foods in the world, is known for being a high fiber, healthy food. Our research has found that commercial popcorn also contains significant amounts of the class of antioxidants known as phenolic acids. The total antioxidant power of raw and popped popcorn

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