11 үр дүн
Supragingival human dental plaque was collected from patients with evidence of caries. The plaque was frozen and stored at -20 degrees C. Pooled plaque was homogenized in acetate buffer pH 5.0 in an ice-water bath. By incubating the homogenate at pH 5.0 with [U-14C]-sucrose the formation of glucose
To analyse carbohydrate metabolism and its role during early seed development of barley we characterised genes encoding two cell wall-bound invertases (HvCWINV1 and HvCWINV2) and two putative hexose transporter-like genes (HvSTP1 and HvSTP2). No typical vacuolar invertase gene could be identified.
Depending on the rats emotional background, unequal invertase and monoglyceride-lipase activity was found which did not correlate with the intensity of synthesis of these enzymes in the enterocytes but depended on the stability or mobility of the enzyme molecules in lipid membranes. The differences
The use of mutants defective in caries-associated traits has enabled the genetic dissociation of agglutination from adhesion, the demonstration of serotype-specific contributions of IPS to virulence, the importance of glucanohydrolase to virulence to a greater degree than to plaque formation, and
Leucrose [D-glucosyl-alpha(1----5)-D-fructopyranose] prepared by microbial-enzymatic transglycosidation from sucrose, is the first alpha(1----5)-linked disaccharide which possesses excellent nutritional properties with regard to metabolic utilization and is well tolerated. The aim of the present
The total effect of sucrose-splitting activity from three sucrose metabolizing enzymes has been investigated in "resting" saliva in contact with dental plaque material in 356 military recruits. Invertase effect is defined as the splitting of sucrose into equimolar quantities of glucose and fructose,
The enzyme that catalyzed the conversion of human salivary alpha-amylase family A (HSA-A) to family B (HSA-B) was identified. It was partially purified from the precipitate obtained by centrifugation of human saliva at 105,000 x g for 60 min by solubilization with
Previous ultrastructural and histochemical analysis proposed patterns in the accumulation of substances in galls of Diptera: Cecidomyiidae in some plant species of the temperate region. Similar analyses were done to verify the conservativeness of these patterns in the Neotropical region, where a