20ml of whole blood will be drawn from each subject each time they are asked to have blood drawn. The total amount of blood collected from subjects will not exceed 360 ml (1.5 cups) within any eight week period throughout the two year timespan patients may be asked to participate in this study.

Acute Humoral Rejections

Chronic Humoral Rejections

Transplant

Patients will be asked to provide blood for immune tests.Patients will not take any study medications, all the research will be performed on blood donated for the study.Patients will donate about 20mls of blood (2 tbls)to be used to determine the subject's blood group and tissue typing (genetic).Results and the patient's contact information will be maintained in a database that is accessible only by the investigators and study personnel conducting the study.For the duration of this study, the investigator will contact patients by phone to request additional samples when needed. Study staff may ask patients donate more blood on short notice (1 day). This will consist of 60-120 ml (6-12 tbls) of blood.The frequency of blood collection will not exceed more than 2 times per week.The total amount of blood collected from subjects will not exceed 360 ml (1.5 cups) within any eight week period throughout the two year timespan patients may be asked to participate in this study.

Blood Draws

Northwestern University Comprehensive Transplant Center

Effects of Thymoglobulin on Human B Cells

Effects of Thymoglobulin on Proliferation, Activation, and Differentiation of Human B Cells

3-6x106 cells of interest will be labeled with 1.5μM CFSE before stimulation. After 6 days, B cells will be recovered, washed, counted and used to define marker settings. The cell phenotype will be analyzed by five-color flow cytometry (FC500, Beckman-Coulter) and by 13-color flow cytometry (Fortessa) and data will be analyzed using CXP software. The supernatant of these B-cell cultures will be using for the cytokines detection, and to determine the number of IgM and IgG producing cells by Luminex.At day 6, cells will be re-stimulated with phorbol 12-myristate 13-acetate (PMA) and Ionomycin, and analyzed by intracellular cytokine staining. Total CD19+ B cells will be sorted into a CD27- (naïve) and CD27+ (memory) B-cell fraction and the phenotypic characterization (assessed by flow cytometry) of these cells will be showed after 6 days of culture with anti-IgM (5μg/ml), anti-CD40 (100ng/ml), and IL-21 (100ng/ml) in the presence or absence of the drugs.

The investigators will co-culture B cells (pre-stimulated with α-IgM and α-CD40 mAb plus IL-21 for 6 days in the presence and absence of Thymoglobulin) with allo-CD4+CD25- T cells (ratio 2:1). After 6 days the investigators will analyze the % of proliferated T-cells and the cytokine production.

Lorenzo Gallon

Professor

Northwestern University

Sanofi

20 cc three times daily for 60 days plus radiation therapy.

Cancer-related Problem/Condition

Head and Neck Cancer

Pain

glutamine

Per institutional standard; minimum of 6,000 cGy with a daily dose of 180-200 cGy.

radiation therapy

l-glutamine placebo

MBCCOP - Gulf Coast

CCOP - Western Regional, Arizona

Veterans Affairs Medical Center - Phoenix (Carl T. Hayden)

Veterans Affairs Medical Center - Tucson

Arizona Cancer Center at University of Arizona Health Sciences Center

Arkansas Cancer Research Center at University of Arkansas for Medical Sciences

Veterans Affairs Medical Center - Little Rock (McClellan)

City of Hope Comprehensive Cancer Center

Veterans Affairs Medical Center - Long Beach

USC/Norris Comprehensive Cancer Center and Hospital

Veterans Affairs Medical Center - West Los Angeles

Jonsson Comprehensive Cancer Center, UCLA

Veterans Affairs Outpatient Clinic - Martinez

CCOP - Bay Area Tumor Institute

Chao Family Comprehensive Cancer Center at University of California Irvine Cancer Center

University of California Davis Cancer Center

UCSF Comprehensive Cancer Center

CCOP - Santa Rosa Memorial Hospital

David Grant Medical Center

University of Colorado Cancer Center at University of Colorado Health Sciences Center

Veterans Affairs Medical Center - Denver

MBCCOP - Howard University Cancer Center

Veterans Affairs Medical Center - Tampa (Haley)

CCOP - Atlanta Regional

Dwight David Eisenhower Army Medical Center

MBCCOP - Hawaii

Tripler Army Medical Center

MBCCOP - University of Illinois at Chicago

Veterans Affairs Medical Center - Chicago (Westside Hospital)

CCOP - Central Illinois

Veterans Affairs Medical Center - Hines (Hines Junior VA Hospital)

Cardinal Bernardin Cancer Center at Loyola University Medical Center

Genesis Regional Cancer Center at Genesis Medical Center

Kansas Masonic Cancer Research Institute at the University of Kansas Medical Center

CCOP - Wichita

Veterans Affairs Medical Center - Wichita

Veterans Affairs Medical Center - Lexington

Markey Cancer Center at University of Kentucky Chandler Medical Center

MBCCOP - LSU Health Sciences Center

Tulane Cancer Center at Tulane University Hospital and Clinic

Veterans Affairs Medical Center - New Orleans

Veterans Affairs Medical Center - Shreveport

Louisiana State University Health Sciences Center - Shreveport

Cancer Research Center at Boston Medical Center

Veterans Affairs Medical Center - Ann Arbor

CCOP - Michigan Cancer Research Consortium

University of Michigan Comprehensive Cancer Center

Barbara Ann Karmanos Cancer Institute

Veterans Affairs Medical Center - Detroit

Josephine Ford Cancer Center at Henry Ford Hospital

CCOP - Grand Rapids

CCOP - Beaumont

Providence Cancer Institute at Providence Hospital

CCOP - Duluth

University of Mississippi Medical Center

Veterans Affairs Medical Center - Jackson

Keesler Medical Center - Keesler Air Force Base

CCOP - Kansas City

St. Louis University Hospital Cancer Center

CCOP - St. Louis-Cape Girardeau

CCOP - Cancer Research for the Ozarks

CCOP - Montana Cancer Consortium

Veterans Affairs Medical Center - East Orange

Veterans Affairs Medical Center - Albuquerque

MBCCOP - University of New Mexico HSC

NYU School of Medicine's Kaplan Comprehensive Cancer Center

Herbert Irving Comprehensive Cancer Center at Columbia University

James P. Wilmot Cancer Center at University of Rochester Medical Center

Veterans Affairs Medical Center - Salisbury

CCOP - Southeast Cancer Control Consortium

Veterans Affairs Medical Center - Cincinnati

Charles M. Barrett Cancer Center at University Hospital

Cleveland Clinic Taussig Cancer Center

CCOP - Columbus

Arthur G. James Cancer Hospital - Ohio State University

Veterans Affairs Medical Center - Dayton

CCOP - Dayton

CCOP - Toledo Community Hospital

Oklahoma University Medical Center

Cancer Institute at Oregon Health and Science University

Veterans Affairs Medical Center - Portland

CCOP - Columbia River Oncology Program

Veterans Affairs Medical Center - Charleston

Hollings Cancer Center at Medical University of South Carolina

CCOP - Greenville

CCOP - Upstate Carolina

Danville Radiation Therapy Center

University of Tennessee Cancer Institute

Harrington Cancer Center

Veterans Affairs Medical Center - Amarillo

Veterans Affairs Medical Center - Dallas

Brooke Army Medical Center

University of Texas Medical Branch

University of Texas - MD Anderson Cancer Center

Veterans Affairs Medical Center - Houston

University of Texas Health Science Center at San Antonio

Veterans Affairs Medical Center - San Antonio (Murphy)

Veterans Affairs Medical Center - Temple

CCOP - Scott and White Hospital

Huntsman Cancer Institute

S9908: Glutamine in Treating Mucositis Caused by Radiation Therapy in Patients With Newly Diagnosed Cancer of the Mouth or Throat

S9908, A Double-Blind, Placebo-Controlled Trial To Study The Efficacy And Safety Of L-Glutamine (In AES0014 Delivery Vehicle) Upon Radiation Therapy-Induced Oral Mucositis In Head and Neck Cancer Patients, Phase III

Evaluate effectiveness of L-glutamine as compared to placebo in terms of: maximum toxicity grade during radiation treatment, mucositis toxicity grade 2 weeks after the end of radiation treatment, and patient reported worst mouth pain two weeks after the end of radiation treatment.

Southwest Oncology Group

National Cancer Institute (NCI)

l glutamine

dental caries

l glutamine/dental caries

Effects of Thymoglobulin on Human B Cells

S9908: Glutamine in Treating Mucositis Caused by Radiation Therapy in Patients With Newly Diagnosed Cancer of the Mouth or Throat

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