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Bladzijde 1 van 183 resultaten
The structure of the rigor complex and its implications for the power stroke.
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Decorated actin provides a model system for studying the strong interaction between actin and myosin. Cryo-energy-filter electron microscopy has recently yielded a 14 A resolution map of rabbit skeletal actin decorated with chicken skeletal S1. The crystal structure of the cross-bridge from skeletal
Adding Rigor to Stroke Rate Investigations in Patients With Atrial Fibrillation.
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Imaging biomarkers in ischemic stroke clinical trials: an opportunity for rigor.
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The post-rigor structure of myosin VI and implications for the recovery stroke.
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Myosin VI has an unexpectedly large swing of its lever arm (powerstroke) that optimizes its unique reverse direction movement. The basis for this is an unprecedented rearrangement of the subdomain to which the lever arm is attached, referred to as the converter. It is unclear at what point(s) in the
Adding rigor to stroke risk prediction in atrial fibrillation.
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Health-related quality of life after stroke a comprehensive review.
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OBJECTIVE
We performed a comprehensive review of all quality-of-life (QOL) estimates for stroke appearing in the peer-reviewed literature between 1985 and 2000. We examine variation in QOL weights and the rigor of methods used to assess QOL and discuss the implications for cost-utility assessment
The power stroke causes changes in the orientation and mobility of the termini of essential light chain 1 of myosin.
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Binding of ATP to the catalytic domain of myosin induces a local conformational change which is believed to cause a major rotation of an 8.5 nm alpha-helix that is stabilized by the regulatory and essential light chains. Here we attempt to follow this rotation by measuring the mobility and
The working stroke upon myosin-nucleotide complexes binding to actin.
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For many years, it has been known that myosin binds to actin tightly, but it had not been possible to devise a muscle fiber experiment to determine whether this binding energy is directly coupled to the working stroke of the actomyosin crossbridge cycle. Addressing the question at the
On the working stroke elicited by steps in length and temperature.
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Muscle contraction is generally thought to involve tilting of the light chain region of the myosin head. This could account for 5-10 nm of axial displacement as it moves from nearly perpendicular to the filament axis (the state at the beginning of the working stroke) to the rigor conformation (at
Quality of the stroke rehabilitation research.
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The Stroke Rehabilitation Evidence-Based Review revealed a wide range of quality scores across primary studies. The aim of this section is to determine what differences there are across studies and to provide a detailed examination of methodological issues in the stroke rehabilitation literature.
The myosin start-of-power stroke state and how actin binding drives the power stroke.
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We propose that on binding to actin at the start of the power stroke the myosin cross-bridge takes on the rigor configuration at the actin interface. Starting from the prepower stroke state, this can be achieved by a small movement (16° rotation) of the lower 50K domain without twisting the central
The association between wind-related variables and stroke symptom onset: A case-crossover study on Jeju Island.
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Although several studies have investigated the effects of ambient temperature on the risk of stroke, few studies have examined the relationship between other meteorological conditions and stroke. Therefore, the aim of this study was to analyze the association between wind-related variables and
Analysis of the quality of clinical practice guidelines on established ischemic stroke.
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OBJECTIVE
To catalogue and comparatively assess the quality of Clinical Practice Guidelines (CPG) for ischemic stroke taking into account format and development methodology.
METHODS
We performed a comprehensive, systematic bibliographic search of CPGs addressing the management of ischemic stroke. We
Reconciling the working strokes of a single head of skeletal muscle myosin estimated from laser-trap experiments and crystal structures.
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Myosin generates force by a rotation of its lever arm. Crystal structures of myosin II indicate an unloaded working stroke of 10-12 nm, a range confirmed by recent x-ray interference experiments. However, when an actin filament, held between two weakly, optically trapped beads is made to interact
Orientation and dynamics of myosin heads in aluminum fluoride induced pre-power stroke states: an EPR study.
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We have determined the orientation and dynamics of the putative pre-power stroke crossbridges in skinned muscle fibers labeled with maleimide spin-label at Cys-707 of myosin. Orientation was measured using electron paramagnetic resonance (EPR) and mobility by saturation transfer EPR. The
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