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Characterization of GDP-Fucose: Polysaccharide Fucosyl Transferase in Corn Roots (Zea mays L.).

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The peripheral root cap cells of corn (cv. SX-17A) secrete a fucose-rich, high molecular weight, polysaccharide slime via the dictyosome pathway. To study the synthesis of this polysaccharide, a technique for isolating and assaying GDP-fucose:polysaccharide fucosyl transferase activity was

Intracellular Localization of GDP-Fucose: Polysaccharide Fucosyl Transferase in Corn Roots (Zea mays L.).

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The intracellular site of synthesis of the fucose-rich polysaccharide slime secreted by corn roots was localized by monitoring the distribution of GDP-fucose:polysaccharide fucosyl transferase activity in subcellular fractions of corn roots. Root tip sections were chopped in the presence of 0.56

Studies on the secretion of maize root-cap slime : III. Histochemical and autoradiographic localization of incorporated fucose.

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Autoradiography was used to localize the sites of incorporation of L-[(3)H]fucose into root tips of maize (Zea mays L. cv. S.X. 17). By light microsocpy, accumulation of label from [(3)H]fucose could be seen in the peripheral cells of the root cap. Extraction of sections prepared by

The detection of fucose residues in plant nuclear envelopes.

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Protoplasts from suspension-culturedDaucus carota L. cells, when fixed and incubated with fluorescein conjugates of the fucosyl-specific lectinUlex europaeus agglutinin I, exhibited the following pattern of labeling: plasma membranes were not marked, but striking halos of fluorescence appeared

Structural Analysis of Secreted Root Slime from Maize (Zea mays L.).

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Secreted slime isolated from the incubation medium of Zea mays roots maintained axenically contains fucose, arabinose, xylose, galactose, and glucose as the major monosaccharides. The slime preparation contains low levels (3% weight/weight [w/w]) of uronic acids. Methylation analysis reveals an

Polyprenyl phosphate sugars synthesized during slime-polysaccharide production by membranes of the root-cap cells of maize (Zea mays).

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Two types of experiments were carried out; either maize roots were incubated in L-[1-3H]fucose or membranes were prepared from root tips and these were incubated with GDP-L-[U-14C]fucose or UDP-D-[U-4C]glucose. The radioactively labelled lipids that were synthesized in vivo and in vitro were

The structure and function of glycoproteins synthesized during slime-polysaccharide production by membranes of the root-cap cells of maize (Zea mays).

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The synthesis of the maize root slime polysaccharides was investigated by using [1-3H]-fucose as a marker for slime production. Three fractions were separated by centrifugation in a CsCl density gradient. Two of these were glycoproteins and occurred within the membranes of the cells of the root tip;

The mucilage secreted by roots and its possible role in cell-cell recognition for the adhesion of fungal pathogens to root surfaces of Zea mays L.

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The outer cells of the roots of plants secrete a mucilage which lubricates the root and keeps it moist. The mucilage is secreted from the Golgi apparatus in vesicles which fuse at the plasma membrane. In maize roots a complex of at least three polysaccharides and glycoproteins are formed, some of

Structural characterization of an arabinoxylan-rhamnogalacturonan complex from cell walls of Zea shoots.

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An arabinoxylan-rhamnogalacturonan complex, comprised of galacturonic acid, rhamnose, arabinose, xylose, and galactose in the ratios 75.9:4.6:5.2:3.5:5.4 and lesser amounts of other constituents, was dissociated from the water-insoluble matrix of cell walls of Zea mays by xylanase and

Dietary fat effects on brush border membrane composition and enzyme activities in rat intestine.

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The effect of dietary fats on the chemical composition and enzyme activities has been studied in intestinal brush border membranes (BBM) or rats. Animals were given commercial rat pellet diet (RP) or semisynthetic diet rich in either saturated [coconut oil (CCO))] or polyunsaturated [n-6, corn oil

The changing fate of a secretory glycoprotein in developing maize endosperm.

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Zeins are the major storage proteins in maize (Zea mays) endosperm, and their accumulation in zein bodies derived from the endoplasmic reticulum is well characterized. In contrast, relatively little is known about post-Golgi compartments or the trafficking of vacuolar proteins in maize endosperm,

First Report of Pantoea agglomerans Causing Leaf Blight and Vascular Wilt in Maize and Sorghum in Mexico.

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Zea mays and Sorghum bicolor are important crops for animal and human nutrition worldwide. In the Central Highland Valley of Mexico, both crops are extremely important, and research is aimed toward increasing yield, disease resistance, and crop adaptation from 1,900- to 2,700-m elevation. In a

Studies on the secretion of maize root cap slime: I. Some properties of the secreted polymer.

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The secreted slime from root cap cells of corn (Zea mays, cv. SX-17) was studied. Production of slime by excised root tips is stimulated by the addition of 40 mM sucrose or fucose and half-strength Hoagland's solution to the incubation medium. Secreted slime was recovered from aqueous solution by

Cytochemical identification of arabinogalactan protein in the outer epidermal wall of maize coleoptiles.

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Artificial carbohydrate antigen (Yariv reagent), fluorescence-labeled α-L-fucose-binding lectin, and β-D-galactose-binding lectin were used to localize arabinogalactan protein in sections of maize (Zea mays L.) coleoptiles. All three probes bind to cell walls of vascular tissue and the outer

Comparative effects of dietary corn, fish and Krill oils on intestinal glycosylation.

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Antarctic Krill is considered as a valuable protein resource for animal and human nutrition. Due to the high content of long chain polyunsaturated fatty acids of the n-3 family, Krill consumption could be also interesting in cardiovascular diseases. In the search for the demonstration of the absence

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