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Transient gene expression of antisense RNA and coat protein-encoding sequences reduced accumulation of cherry leafroll virus in tobacco protoplasts.

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Cherry leafroll nepovirus (CLRV) is the causative agent of blackline disease, which results in a fatal necrosis of the graft union of English walnut scion on certain rootstocks. Tobacco suspension cell protoplasts were electroporated with plasmid constructions, bearing or not bearing RNA-derived

Mechanism of gallic acid biosynthesis in bacteria (Escherichia coli) and walnut (Juglans regia).

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Gallic acid (GA), a key intermediate in the synthesis of plant hydrolysable tannins, is also a primary anti-inflammatory, cardio-protective agent found in wine, tea, and cocoa. In this publication, we reveal the identity of a gene and encoded protein essential for GA synthesis. Although it has long

First Report of Seedborne Cherry leaf roll virus in Wild Potato, Solanum acaule, from South America.

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A virus, designated JCM-79, was isolated from wild potato (Solanum acaule Bitt.) plants grown from true seed received at USDA-APHIS Potato Quarantine Program from Peru. JCM-79 was mechanically transmissible to Nicotiana clevelandii and N. tabacum cv. Samsun NN. Symptoms in the original S. acaule

First findings of plum pox virus in walnut trees (Juglans Regia L.)

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Plum pox virus (PPV) was transmitted from infected buds and leaves of walnut tree (Juglans regia L.) on the following herbaceous indicators: Chenopodium foetidum Schrad., Nicotiana bigelovii var. quadrivalvis Fuchs., N. clevelandii x N. glutinosa. A positive ELISA reaction with antisera against PPV

Acetylcholine/dopamine interaction in planaria.

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Planaria represents the most primitive example of centralization and cephalization of nervous system. Previous reports indicate that planaria shows specific behavioral patterns, analogous to mammalian stereotypes, in response to drugs acting on acetylcholine or dopamine transmission. Here we further

First Report of Bacterial Bark Canker of Walnut Caused by Brenneria nigrifluens in Hungary.

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During August 2012, vertical oozing cankers were sporadically observed on trunks and branches of walnut trees (Juglans regia) in the city of Zánka, near Lake Balaton and other parts of Hungary including Budapest, Győr, and Tatabánya cities. Cankers were observed on trunks and branches where

Genetic loci involved in rubrifacine production in the walnut pathogen Brenneria rubrifaciens.

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Brenneria rubrifaciens produces a unique red pigment known as rubrifacine that has been hypothesized to play a role in pathogenesis on walnut. Analysis of DNA flanking the Tn5 insertion site in 20 rubrifacine minus (pig(-)) mutants identified three regions required for rubrifacine production. The

First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.

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In late summer 2011, shallow, irregular cankers were observed on trunks and branches of non-chemically-treated walnut trees (Juglans regia L.) on a 30-year-old orchard in the region of Fruška Gora (Vojvodina, Serbia). Disease incidence was ~80% and yield loss was ~50%. For pathogen isolation, small

Multiple transcriptional regulation of walnut JrGSTTau1 gene in response to osmotic stress.

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Glutathione S-transferases (GSTs) are important plant proteins involved in biotic and abiotic stress responses. A gene from Juglans regia, JrGSTTau1 was previously cloned and functionally characterized as an enzyme involved in improving cold tolerance in plants. To clarify the functional mechanism

In planta characterization of a tau class glutathione S-transferase gene from Juglans regia (JrGSTTau1) involved in chilling tolerance.

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CONCLUSIONS JrGSTTau1 is an important candidate gene for plant chilling tolerance regulation. A tau subfamily glutathione S-transferase (GST) gene from Juglans regia (JrGSTTau1, GeneBank No.: KT351091) was cloned and functionally characterized. JrGSTTau1 was induced by 16, 12, 10, 8, and 6 °C

The effect of Maras powder and smoking on the microRNA deregulation of oral mucosa.

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This study aimed to investigate the effects of Maras powder (a type of smokeless tobacco obtained from Nicotiana rustica Linn and mixed with the ashes of wood, especially from oak, walnut or grapevine) on the microRNA (miRNA) deregulation of oral mucosa, and it compares these effects

[Comprehensive benefits of agroforestry snail control forests in Eryuan County, Yunnan Province]

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Objective: To investigate the efficiency of various agroforestry systems for snail control in plateau hilly schistosomiasis-endemic areas of Yunnan Province, so as to provide insights into the construction of agroforestry schistosomiasis

Slow-pyrolysis and -oxidation of different biomass fuel samples.

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Pyrolysis and oxidation characteristics of some biomass samples such as almond shell, walnut shell, hazelnut shell, tobacco waste, and rapeseed were investigated using Thermogravimetric Analysis (TGA) technique under slow heating conditions (20 K/min) from ambient to 1173 K. Pyrolysis experiments

Performance of the piezoelectric microbalance respirable aerosol sensor.

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A battery-portable respirable aerosol sensor has been experimentally evaluated. Calibration procedures are described. For instruments calibrated with welding smoke, test results show agreement within +/- 15% of parallel filter samples for 10 laboratory and industrial aerosols including welding and

Diet and diabetes: lines and dots.

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Diabetes, particularly type 2 diabetes, is epidemic in the United States among adults and children alike, and increasingly prevalent around the world. On its current trajectory, the increasing incidence of diabetes has the potential to ravage both public health and economies. There has, however,

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