include 40 premenopausal women (age ˂ 45 year) with uterine fibroids who are diagnosed through clinical gynecological, ultrasound and other auxiliary examinations.
The protein expression of the followings markers will be estimated in tumor tissue samples:
Fibroblast activation protein (FAP) will be measured by quantitative real time polymerase chain reaction qRTPCR (mRNA level) and ELISA (protein level).
Autophagy markers level (LC3 and p62) will be measured by qRTPCR (mRNA level) and by immunohistochemical analysis.
Phosphorylated protein kinase B (pAKT) level will be measured by western blot analysis.
Circulating (cFAP) will be measured using ELISA in blood of UF patients.

include 40 (age, BMI) matched healthy females
The protein expression followings markers will be estimated in normal myometrial tissue samples( 1cm from UF lesions):
Fibroblast activation protein (FAP) will be measured by quantitative real time polymerase chain reaction qRTPCR (mRNA level) and ELISA (protein level).
Autophagy markers level (LC3 and p62) will be measured by qRTPCR (mRNA level) and by immunohistochemical analysis.
Phosphorylated protein kinase B (pAKT) level will be measured by western blot analysis.
Circulating (cFAP) will be measured using ELISA in blood of healthy controls

2 types of samples will be collected : - Blood samples : will be collected from the patient and control groups - Tissue samples: will be collected from patient group who will be scheduled to undergo myomectomy or hysterectomy due to symptomatic UF. Tissue samples include tumor tissue samples and normal myometrial tissue samples from the area (1cm from the uterine fibroids lesions).

Uterine Fibroid

The followings markers will be estimated in tissue, and/ or blood samples:
Fibroblast activation protein (FAP) will be measured by quantitative real time polymerase chain reaction qRTPCR (mRNA level) and ELISA (protein level).
Circulating (cFAP) will be measured using ELISA in blood of UF patients and healthy controls.
Autophagy markers level (LC3 and p62) will be measured by qRTPCR (mRNA level) and by immunohistochemical analysis in both UF tissue samples and normal myometrial tissues.
Phosphorylated protein kinase B (pAKT) level will be measured by western blot analysis.

Measurement of protein expression in tissue and /or blood samples.

Assiut university -Faculty of medicine- Departement of Biochemistry

The Role of Fibroblast Activation in Uterine Fibroid

A Novel Insight Into the Role of Fibroblast Activation in Uterine Fibroid

Explore difference in level of fibroblast activation marker (FAP) and autophagy markers (LC3 and P62) in UF tissue samples compared to normal myometrial samples (1 cm from the UF lesion). Detect difference of cFAP levels in blood of UF patients and healthy controls.

Investigate the correlation between fibroblast activation and autophagy through PI3/AKT signaling pathway and their role in the pathogenesis of UF through estimation of AKT protein expression in UF tissue samples compared to normal myometrial samples 1 cm from the UF lesion.

leiomyoma/protease

The Role of Fibroblast Activation in Uterine Fibroid

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