mevalonic acid/nicotiana

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Incorporation of mevalonic Acid into ribosylzeatin in tobacco callus ribonucleic Acid preparations.

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The incorporation of (14)C-2-mevalonic acid into transfer RNA and ribosomal RNA (high molecular weight RNA) in rapidly growing, cytokinin-dependent tobacco (Nicotiana tabacum var. Wisconsin No. 38) callus cultures has been investigated. Approximately 40% of the label incorporated into transfer RNA

Effect of Light on Mevalonic Acid Incorporation into the Phytosterols of Nicotiana tabacum L. Seedlings.

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Mevalonic acid-2-(14)C was readily incorporated into the free, esterified, and glycosidic sterol fractions of tobacco (Nicotiana tabacum L. var. Burley 21) seedlings. The time course of mevalonic acid-2-(14)C incorporation was different for the various individual sterols. Campesterol and sitosterol

Environmental and Genetic Factors Associated with Solanesol Accumulation in Potato Leaves.

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Solanesol is a high value 45-carbon, unsaturated, all-trans-nonaprenol isoprenoid. Recently solanesol has received particular attention because of its utility, both in its own right and as a precursor in the production of numerous compounds used in the treatment of disease states. Solanesol is found

Effects of mevinolin on cell cycle progression and viability of tobacco BY-2 cells.

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Mevinolin, an inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase, was used to study the importance of mevalonic acid (MVA) for cell cycle progression of tobacco (Nicotiana tabacum L.) BY-2 cells. After treatment with 5 microM mevinolin, the cell cycle progression was completely blocked and two

Plant Sterol Diversity in Pollen from Angiosperms.

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Here we have examined the composition of free sterols and steryl esters of pollen from selected angiosperm species, as a first step towards a comprehensive analysis of sterol biogenesis in the male gametophyte. We detected four major sterol structural groups: cycloartenol derivatives bearing a

Inhibition of growth of cultured tobacco cells at low concentrations of lovastatin is reversed by cytokinin.

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De novo synthesis of mevalonic acid, which is catalyzed by 3-hydroxy-3-methylglutaryl coenzyme A reductase, is the first committed step in the formation of isoprenoid compounds. Various studies have shown that mevalonic acid-derived compounds are required for growth of plant and animal cells, a

Plant growth retardants as inhibitors of sterol biosynthesis in tobacco seedlings.

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Three plant-growth retardants 2'-isopropy1-4'-(trimethylammonium chloride)-5-methylphenylpiperidine carboxylate (Amo 1618), beta-chloroethyltrimethylammonium chloride, and tributyl-2, 4-dichlorobenzylphosphonium chloride were tested for their effects on sterol production in, and growth of tobacco

Effect of tobacco mosaic virus infection on glucose metabolism in Nicotiana tabacum var. Samsun. III. Investigation of hexosemonophosphate shunt enzymes and steroid concentration and biosynthesis.

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Activity of the enzymes phosphoriboisomerase, glucose-6-phosphate and 6-phosphogluconic acid dehydrogenases were assayed in healthy and TMV-infected roots of Nicotiana tabacum L. var. Samsun plants grown in nutrient culture. The results indicate that all enzymes were equally active in both infected

Zeatin is indispensable for the G2-M transition in tobacco BY-2 cells.

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The importance of N6-isoprenoid cytokinins in the G2-M transition of Nicotiana tabacum BY-2 cells was investigated. Both cytokinin biosynthesis and entry in mitosis were partially blocked by application at early or late G2 of lovastatin (10 microM), an inhibitor of mevalonic acid synthesis. LC-MS/MS

Production of santalenes and bergamotene in Nicotiana tabacum plants.

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Terpenes play an important role in plant-insect relationships, and these relationships can potentially be modified by altering the profile of terpenes emitted from plants using metabolic engineering methods. Transgenic plants generated by employing such methods offer the prospect of low-cost

S-carvone suppresses cellulase-induced capsidiol production in Nicotiana tabacum by interfering with protein isoprenylation.

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S-Carvone has been described as a negative regulator of mevalonic acid (MVA) production by interfering with 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMGR) activity, a key player in isoprenoid biosynthesis. The impact of this monoterpene on the production of capsidiol in Nicotiana tabacum,

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