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A cultivation-independent approach was used to identify potentially nitrogen-fixing endophytes in seven sweet potato varieties collected in Uganda and Kenya. Nitrogenase reductase genes (nifH) were amplified by PCR, and amplicons were cloned in Escherichia coli. Clones were grouped by restriction
Seventeen strains of Spirillum-like organisms (2) were isolated from the Parana Delta and other rivers (Table 1), using the following medium: 0.5% malic acid; 0.4% KOH; 0.5% K2HPO4; 0.005% yeast extract; pH was adjusted to 7 with KOH and 0.15% agar. The organisms produced a white, dense and
Azotobacters have been used as biofertilizer since more than a century. Azotobacters fix nitrogen aerobically, elaborate plant hormones, solubilize phosphates and also suppress phytopathogens or reduce their deleterious effect. Application of wild type Azotobacters results in better yield of cereals