4 resultaten
Cotton (Gossypium hirsutum) seeds and Sorghum vulgare caryopses are able to incorporate CO2 through a PEP-carboxylating enzyme (EC 4.1.1.38). The enzyme activity is optimal at pH 8.2 and is unaffected by ATP, GDP or acetyl CoA. The partially purified cotton enzyme is stimulated by inorganic
The expression of a Sorghum vulgäre gene encoding the phosphoenol-pyruvate carboxylase involved in C4 photosynthesis has been studied after introduction into tobacco. Northern blot analysis of poly(A) mRNA from green leaves demonstrated i) the efficiency of this monocot promoter, ii) the
The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is
The ambiguous location of photosynthetic carboxylases of mesophyll and bundle sheath chloroplasts of sorghum was investigated after successful homogeneous preparation. The phosphoenol pyruvate carboxylase was found as a particulate enzyme in the mesophyll cell chloroplasts and ribulose