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Side 1 fra 78 resultater
Utility of pentose colorimetric assay for the purification of potato lectin, an arabinose-rich glycoprotein.
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Potato lectin (Solanum tuberosum agglutinin, STA) is an unusual glycoprotein containing approximately 50% carbohydrates by weight. Of the total carbohydrates, 92% is contributed by L: -arabinose, which are O-linked to hydroxyproline residues. The ferric chloride-orcinol assay (Bial's test), which is
Effects of feeding diets containing lactose, agar, cellulose, raw potato starch or arabinose on the dry weights of cleaned gastrointestinal tract organs in the rat.
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The sugar composition and partial structure of the self-induced endogenous elicitor from potato.
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The self-induced endogenous elicitor isolated from potato having molecular weight 9200 was degraded with trifluoroacetic acid to determine the sugar composition. The requisite composition of the elicitor was estimated to be a 2:3:4:7 mixture of galactose, rhamnose, arabinose, and galacturonic acid.
Enzymatic generation of galactose-rich oligosaccharides/oligomers from potato rhamnogalacturonan I pectic polysaccharides.
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Potato pulp by-product rich in galactan-rich rhamnogalacturonan I (RG I) was investigated as a new source of oligosaccharides with potential prebiotic properties. The efficiency of selected monocomponent enzymes and multi-enzymatic preparations to generate oligosaccharides/oligomers from potato RG I
Affecting osteoblastic responses with in vivo engineered potato pectin fragments.
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Pectins, complex plant-derived polysaccharides, are novel candidates for biomaterial nanocoatings. Pectic rhamnogalacturonan-I regions (RG-I) can be enzymatically treated to so-called modified hairy regions (MHR). We surveyed the growth and differentiation of murine preosteoblastic MC3T3-E1 cells on
The location of arabinosyl:hydroxyproline transferase in the membrane system of potato tissue culture cells.
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Incubation of a particulate preparation from potato tissue culture cells with UDP-beta-L-[1-3H] arabinose yielded a glycoprotein fraction containing labelled material with the characteristics of hydroxyproline arabinosides. The sugar-protein linkage was resistant to hot alkaline hydrolysis, and the
The polysaccharide structure of potato cell walls: Chemical fractionation.
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Cell walls of potato tubers were fractionated by successive extraction with various reagents. A slightly degraded pectic fraction with 77% galacturonic acid was extracted in hot, oxalate-citrate buffer at pH 4. A further, major pectic fraction with 38% galacturonic acid was extracted in cold 0.1 M
Properties of potato lectin and the nature of its glycoprotein linkages.
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1. Potato lectin is a glycoprotein that contains about 47% (by weight) l-arabinose, 3% d-galactose and 11% hydroxyproline. It has a monomeric molecular weight of about 50000 and probably exists as a monomer-dimer system in aqueous solution, with the monomer predominating. It has a very high
The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein.
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1. Potato lectin has been purified and shown to be a glycoprotein containing about 50% of carbohydrate. Most of the sugar residues (92%) are arabinose; small amounts of galactose, glucose and glucosamine are also present. 2. The most abundant amino acid is hydroxyproline (16% of the residues), 11.5%
Analysis of the Components Released from Potato Tuber Tissues during Maceration by Pectolytic Enzymes.
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Endo-pectin lyase and endo-polygalacturonase of Aspergillus japonicus attack the middle lamella of plant tissue and cause tissue maceration. Galacturonides, neutral sugars, and proteins were released from potato tuber tissues during maceration by both purified enzymes. These three components
Enzymatic hydrolysis of potato pulp.
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BACKGROUND
Potato pulp constitutes a complicated system of four types of polysaccharides: cellulose, hemicellulose, pectin and starch. Its composition makes it a potential and attractive raw material for the production of the second generation bioethanol. The aim of this research project was to
Effects of sulphur, nitrogen, phosphorus, potassium, and water stress on dietary fibre fractions, starch, amino acids and on the biological value of potato protein.
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In pot experiments with greatly differing rates of N, P, K, and S, and 3 levels of water, dry matter (DM) yields of tubers varied from 28 to 454 g/pot. Especially P-, K- and S-deficiency reduced the starch content of boiled potatoes, from P from 74 to 59% in DM. S-deficiency increased soluble,
Purification and characterization of potato lectin.
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Potato lectin (Solanum tuberosum agglutinin, STA), purified by affinity chromatography on tri-N-acetylchitotriose-Sepharose 6B, has Mr approximately 100,000, as estimated by gel filtration on Sephadex G-150 and is an aggregating system with a monomer Mr = 54,000, as estimated by sedimentation
Composition and physicochemical properties of dietary fiber extracted from residues of 10 varieties of sweet potato by a sieving method.
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Dietary fiber (DF) was extracted from sweet potato residues after starch isolation of 10 varieties using a sieving method. The proximate composition of sweet potato residues, chemical composition, monosaccharide composition, and physicochemical properties of DF were investigated. The average yield
Control of starch and exocellular polysaccharides biosynthesis by gibberellic acid with cells of sweet potato cultured in vitro.
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The regulation of starch synthesis and exocellular polysaccharide synthesis by GA3 was studied with cells of sweet potato grown as suspension in glycerol medium. In the presence of GA3, and under normal cell growth, starch formation was inhibited. The incorporation activity (starch synthesis) from
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