digitalis/phosphatase

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Interaction of (Na+,K+)-ATPases and digitalis genins. A general model for inhibitory activity.

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Previous models of digitalis genin interaction with the (Na+,K+)-ATPase system (the putative receptor for such drugs) were deficient in explaining the (Na+,K+)-ATPase inhibitory activity of a number of digitalis genin analogues. With rat brain (Na+,K+)-ATPase we observed that the C-17 side chain

[A case of dilated cardiomyopathy caused by myocardial microinfarcts in the course of polycythemia vera].

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This article presents a case of 45-year-old man with polycythemia vera non diagnosed before. The first symptom of polycythemia vera was acute congestive heart failure which suggested diagnosis of myocarditis. Polycythemia vera was confirmed by raised hematocrit, significantly increased platelet

The age-dependent changes in the number of 3H-ouabain binding sites in mammalian skeletal muscle.

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The influence of age on the binding of 3H-ouabain in skeletal muscle has been characterized in rats, mice and guinea pigs. Measurements performed using biopsies and intact fibers obtained from different types of rat muscles showed that from birth to the 4th week of life, the number of 3H-ouabain

Consequences of mutations to the phosphorylation site of the alpha-subunit of Na, K-ATPase for ATP binding and E1-E2 conformational equilibrium.

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Expression of Na, K-ATPase in yeast allowed targeting of alpha beta-units with lethal substitutions at the phosphorylation site alpha 1 (D369N) beta 1 and alpha 1 (D369A) beta 1 at the cell surface at the same concentration of alpha-subunit and [3H] ouabain binding sites as for wild type Na,

A case of advanced breast cancer associated with hypocalcemia.

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Although hypercalcemia is a well-known complication of malignant diseases, hypocalcemia seems to be a rather rare one. A 34-yr-old woman with advanced breast cancer who presented hypocalcemia is described. She had generalized multiple osteolytic bone metastases which were progressive in spite of

Myocardial Na+,K(+)-ATPase in tachycardia induced cardiomyopathy.

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Na+,K(+)-ATPase is a major determinant of myocyte homeostasis and excitation-contraction. Cardiac glycosides such as digitalis and ouabain increase the inotropic state of the heart through the inhibition of Na+,K(+)-ATPase. While cardiac glycosides are commonly used in the setting of congestive

1Nonradioactive In Situ Hybridization in Atherosclerotic Tissue.

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In situ hybridization (ISH) is a powerful and important technique that allows the detection and microscopic localization of nucleic acids within the specific cell, tissue, or chromosome of interest. In addition, it offers increased sensitivity over traditional filter hybridization, since low-copy

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