Identifying the Anti-Blood-Clotting Compounds in Garlic
Słowa kluczowe
Abstrakcyjny
Opis
Published studies indicate that various types of undefined garlic products display antiplatelet activity. However, the compounds responsible for this antiplatelet effect have not been identified.
Acute studies. In acute (one day) studies, healthy participants will consume several doses of "fresh garlic" (garlic powder added to ambient water; up to the equivalent of 12 grams fresh garlic or 52 mg allicin) as a paste in a tuna sandwich. At 0, 2, 4, and 6 hours after consumption, the ability of platelets to aggregate in platelet-rich plasma (PRP) or in whole blood (WB), in response to a drug (collagen, ADP) that stimulates platelet aggregation, will be measured. After establishing the optimum dose and optimum time of the antiplatelet effect for each person, and if the effects are sufficiently strong, the effects of cooked garlic (garlic powder added to boiling water, no allicin present) and seven extracted fractions (oil, protein, fructans, etc.) will be determined, using the fractions at the same dose as their abundance in the established optimum dose of crushed fresh garlic.
Chronic studies. If only weak platelet effects are found for a high acute dose of "fresh" garlic, chronic studies will be conducted. In chronic studies, participants will consume 8.0 grams of "fresh garlic" (2.7 grams garlic powder added to ambient water, allicin content = 35 mg) as a paste in a tuna sandwich every day for four weeks and 8.0 grams of "boiled garlic" (2.7 grams garlic powder added to boiling water) in a sandwich every day for an additional four weeks, after a 1-week washout. The ability of platelets to aggregate in PRP and whole blood will be determined before and every week after garlic consumption begins and after the 1-week washout.
Daktyle
| Ostatnia weryfikacja: | 11/30/2009 |
| Pierwsze przesłane: | 09/11/2005 |
| Szacowana liczba przesłanych rejestracji: | 09/11/2005 |
| Wysłany pierwszy: | 09/19/2005 |
| Ostatnia aktualizacja przesłana: | 12/03/2009 |
| Ostatnia opublikowana aktualizacja: | 01/10/2010 |
| Data pierwszego przesłania wyników: | 04/22/2009 |
| Data pierwszego przesłania wyników kontroli jakości: | 12/03/2009 |
| Data opublikowania pierwszych wyników: | 01/10/2010 |
| Rzeczywista data rozpoczęcia badania: | 02/28/2006 |
| Szacowana data zakończenia podstawowej działalności: | 08/31/2007 |
| Szacowana data zakończenia badania: | 08/31/2007 |
Stan lub choroba
Interwencja / leczenie
Dietary Supplement: Garlic powder in ambient water
Dietary Supplement: garlic powder in boiling water
Faza
Grupy ramion
| Ramię | Interwencja / leczenie |
|---|---|
| Active Comparator: Garlic powder in ambient water high allicin | Dietary Supplement: Garlic powder in ambient water consumed 2.7 grams of garlic powder added to ambient water (equivalent to 8 grams fresh or raw garlic) in a sandwich, once a day for four weeks |
| Experimental: garlic powder in boiling water no allicin | Dietary Supplement: garlic powder in boiling water consumed 2.7 grams of garlic powder added to boiling water (equivalent to 8 grams of cooked garlic) in a sandwich, once a day for four weeks |
Kryteria kwalifikacji
| Wiek kwalifikujący się do nauki | 18 Years Do 18 Years |
| Płeć kwalifikująca się do nauki | All |
| Przyjmuje zdrowych wolontariuszy | tak |
| Kryteria | Inclusion Criteria: - Body mass index of 19 to 30 kg/m2 - Plans to remain in the study area for the next year - Willingness to abstain from nonsteroidal anti-inflammatory drugs (NSAID drugs for 1 week prior to each study test - Willingness to abstain from consuming garlic and significant amounts of onion, chocolate, or purple grape juice for 3 days prior to each study test - Willingness to participate in all study tests Exclusion Criteria: - Pregnancy or breast-feeding - Serious medical condition - Allergy to garlic or wheat - Tobacco use - Excessive alcohol consumption - Under psychiatric care |
Wynik
Podstawowe miary wyników
1. Percent Platelet Aggregation [4 weeks]
Miary wyników wtórnych
1. Electrical Impedance in Ohms [4 weeks]
