The Role of SCUBE-1 in Ischemia-reperfusion Injury

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StatusZakończony

Sponsorzy

Diskapi Yildirim Beyazit Education and Research Hospital

BADANIE KLINICZNE: NCT03389607

BioSeek: nct03389607

Słowa kluczowe

Abstrakcyjny

One consequence of tissue damage caused by tourniquet is ischemia-reperfusion injury. Short-term ischemia leads to vasodilatation and reactive hyperemia resulting in post-ischemic reperfusion microcirculation failure and tissue edema that extends from 30 minutes to 4 hours.

SCUBE-1 is a newly defined cell surface molecule. It emerges from many developing cells, including endothelium and platelets. Immunohistochemical demonstration of subendothelial matrix deposition in atherosclerosis in humans. We did not find any study that showed the post-ischemic regression of scube 1, which was shown to be significantly higher in ischemic events in the literature.

in this study is aimed to investigate the location / sensitivity of SCUBE-1 in diabetics and nondiabetics after application of regional anesthesia for ischemia-reperfusion injury induced by tourniquet application in knee prosthesis attempts in our aimed patients and compare this with other total antioxidant status (TAS) and MDA of ischemia-reperfusion parameters

Opis

After being taken to the operation room, the patient will be divided into two groups as group D (diabetic) and group K (control) by standardized anesthesia monitoring with 5-lead electrocardiogram (ECG), peripheral oxygen saturation (SpO2) and noninvasive blood pressure measurements.

In both groups, spinal anesthesia will be applied in a lateral decubitus position using a 22 gauge Quincke spinal needle from the interspinal space as 0.5% heavy marcaine 10-12.5 mg 1 min. Atropine 0.5 mg and ephedrine 5 mg should be administered if bradycardia develops in both groups. The disease tourniquet will be applied after induction, in accordance with the literature, to be above 150 mmHg of the systolic blood pressure.

Daktyle

Ostatnia weryfikacja:	09/30/2019
Pierwsze przesłane:	12/25/2017
Szacowana liczba przesłanych rejestracji:	12/25/2017
Wysłany pierwszy:	01/02/2018
Ostatnia aktualizacja przesłana:	10/06/2019
Ostatnia opublikowana aktualizacja:	10/08/2019
Rzeczywista data rozpoczęcia badania:	01/07/2018
Szacowana data zakończenia podstawowej działalności:	08/31/2018
Szacowana data zakończenia badania:	01/31/2019

Stan lub choroba

Gonarthrosis

Diabetes

Interwencja / leczenie

Diagnostic Test: spinal anesthesia

Faza

Grupy ramion

Ramię	Interwencja / leczenie
diabetic the patients must have diabetic disease
control the patients must not have diabetic

Kryteria kwalifikacji

Wiek kwalifikujący się do nauki	18 Years Do 18 Years
Płeć kwalifikująca się do nauki	All
Metoda próbkowania	Probability Sample
Przyjmuje zdrowych wolontariuszy	tak
Kryteria	Inclusion Criteria: - Diabetes mellitus - knee replacement surgery - using a pneumatic tourniquet Exclusion Criteria: - coronary artery disease - renal disorders - cognitive disorders - bleeding diathesis - who have passed general anesthesia - anti-inflammatory drugs treatment - abnormal HbA1c values

Wynik

Podstawowe miary wyników

1. SCUBE-1 [before spinal anesthesia, before 5 min opening of tourniquet and 2 hr after operation]

mean level of SCUBE-1

Miary wyników wtórnych

1. malondialdehyde (MDA) [before spinal anesthesia, before 5 min opening of tourniquet and 2 hr after operation]

mean level of MDA

2. total antioxidant capacity [before spinal anesthesia, before 5 min opening of tourniquet and 2 hr after operation]

mean level of total antioxidant capacity (TAC)

The Role of SCUBE-1 in Ischemia-reperfusion Injury

Słowa kluczowe

efedryna

niedokrwienie

obrzęk

bradykardia

miażdżyca

atropina

przeciwutleniacz