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Protoplasts isolated from a totipotent embryogenic cell suspension culture of Zea mays L. (cultivar 'Dekalb XL82') underwent sustained cell divisions when cultured in liquid as well as agarose media. Optimal colony formation (5%) occurred in a liquid medium containing 0.5 mg/l
Agar blocks that contacted the upper sides of tips of horizontally-oriented roots of Zea mays contain significantly less calcium (Ca) than blocks that contacted the lower sides of such roots. This gravity-induced gradient of Ca forms prior to the onset of gravicurvature, and does not form across
We placed agar blocks adjacent to tips of electrotropically stimulated primary roots of Zea mays. Blocks placed adjacent to the anode-side of the roots for 3 h induced significant curvature when subsequently placed asymmetrically on tips of vertically-oriented roots. Curvature was always toward the
Kernels of Zea mays on an intact plant accumulate indole-3-acetic acid (IAA) at the rate of 190 ng g-1 fresh weight h-1. Of the IAA synthesized, 97% is in the esterified form and less than 3% remains as the free acid. The site of biosynthesis of the IAA, whether synthesized in the leaf and
Primary roots of Zea mays cv. Yellow Dent growing in an electric field curve towards the anode. Roots treated with EDTA and growing in electric field do not curve. When root cap mucilage is applied asymmetrically to tips of vertically-oriented roots, the roots curve toward the mucilage. Roots
Indole-3-acetic acid-1'-(14)C (IAA-(14)C) and tryptophan-1-(14)C injected in small amounts into cotyledons of Phaseolus coccineus L. seedlings were found to be translocated acropetally into the epicotyls and young shoots. Similarly IAA-(14)C was translocated acropetally into coleoptiles of Zea mays
Growth of lateral roots is regulated by both environmental factors and nitrate (NO(-)(3)) content of the plant. The mechanism involved is not clearly understood. Two maize (Zea mays L.) inbred lines (478 and Wu312) were used to study the effect of different nitrate supply on lateral root (LR) growth
During a study of microorganisms associated with maize (Zea mays) cultivation, yeasts were isolated from overwintered stalks, cobs and surrounding soil, which were collected from an agricultural field in south-central Illinois, USA. Predominant among isolates were two species of Cryptococcus (Cr.
The Escherichia coli phytase gene appA encoding enzyme AppA was cloned in a broad host range plasmid pBBR1MCS2 (lac promoter), termed pVA1, and transformed into the Ensifer meliloti 1020. Transformation of pVA1 in Ensifer meliloti {E. m (pVA1)} increased its phosphatase and phytase activity by ∼9-
We investigated the interaction of Ca2+ and pH on root elongation in Zea mays L. cv. B73 x Missouri 17 and cv. Merit. Seedlings were raised to contain high levels of Ca2+ (HC, imbibed and raised in 10 mM CaCl2) or low levels of Ca2+ (LC, imbibed and raised in distilled water). In HC roots, lowering
In August 2008, long and narrow lesions were observed on leaves of corn (Zea mays L.) growing in a field in Pope County, Illinois. Lesions were 10 to 35 × 50 to 250 mm and were cream to tan. Dark pycnidia inside the lesions were immersed and approximately 350 μm in diameter. Affected leaves were
Red root rot, caused by Phoma terrestris E. M. Hansen, caused premature senescence and yield reductions to fresh-market sweet corn in Hyde County, North Carolina in July 2006. Foliar symptoms developed over a period of 5 to 8 days approximately 1 to 2 weeks after anthesis and included desiccation of
Diffusible gibberellins were obtainable in agar from excised 4 mm tips of etiolated coleoptiles of Zea mays. Placing the tips in a horizontal position increased the total yield of gibberellins by approximately five times. With horizontal tips, the ratio of gibberellin activity recovered from lower
Sugar release from the pedicel tissue of maize (Zea mays L.) kernels was studied by removing the distal portion of the kernel and the lower endosperm, followed by replacement of the endosperm with an agar solute trap. Sugars were unloaded into the apoplast of the pedicel and accumulated in the agar
Phenolic compounds, like carvacrol, in oregano interfere with the detection of foodborne pathogens such as Salmonella enterica. Carvacrol concentration varies based on plant cultivars and growth region. Six oregano cultivars were used to compare the impact of carvacrol concentration on Salmonella