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The polymorphic mononuclear cells, arranged in whorling or palisading pattern, were usually found in the lung of Actinobacillus pleuropneumoniae-infected pigs. In order to understand the origin and characteristics of these cells, specific-pathogen free pigs were intratracheally inoculated with
Spiroplasma citri was cultured in three different media that supplied cholesterol and fatty acids from: (i) horse serum, (ii) pleuropneumonia-like organism (PPLO) serum fraction, or (iii) bovine serum albumin-fatty acid-cholesterol. The ability of PPLO serum fraction to support growth varied by lot
The gram-negative bacterium Actinobacillus pleuropneumoniae (APP) is an inhabitant of the porcine upper respiratory tract and the causative agent of porcine pleuropneumonia (PP). In recent years, knowledge about the proinflammatory cytokine and chemokine gene expression that occurs in lung and lymph