Alkaloids from the leaves of Daphniphyllum subverticillatum.

Four new alkaloids, 17-hydroxydaphnigraciline (1), subdaphnidine A (2), daphnezomine L methyl ester (3), and 11-hydroxycodaphniphylline (4), along with 24 known analogues, were isolated from the leaves of Daphniphyllum subverticillatum. The structures of 1-4 were elucidated on the basis of spectroscopic methods and from chemical evidence. Daphnilongeridine (5) showed cytotoxicity against several tumor cell lines at IC(50) values in the range 2.4-9.7 microM and against the HMEC human microvascular endothelial cell line with an IC(50) of 2.7 microM.