[Morphological changes in liver tissue in experimental porphyria].

Miscroscopic changes in the liver tissue of mice became detectable 42 hours after the administration of gricine through the appearance of porphyrins in the plasma of hepatocytes marked by the enlargement of their volume, nuclei, and nucleoli. 72 hours after gricine, porphyrins were also found in the lumina of capilary bile ducts and hepatic ducts. This was accompanied by dispersion extinction of hepatocytes and pigment accumulation in the plasma of Kupffer's cells. There was histochemically proved increased activity of alkaline and acidic phosphatase, G6PDH, NADH and NADPH tetrazolium reductase. Under electron microscopy, the porphyrins showed crystalline structure, rotated the plane of polarized light, and were marked by red fluorescence.