Synthesis, Characterization and Antimicrobial Activity of Garcinol Capped Silver Nanoparticles.

Garcinol, a well-known medicinal phytochemical, was extracted and isolated from the dried fruit rinds of Garcinia quaesita Pierre. In this study, garcinol has successfully used to reduce silver ions to silver in order to synthesize G-AgNPs. The formation and the structure of G-AgNPs were confirmed by UV-Visible spectroscopy, FTIR spectroscopy and TEM. The antimicrobial activity of garcinol and G-AgNPs were investigated by well diffusion assay, broth micro dilution assay and time-kill kinetic study, over selected five microbial species including Staphylococcus aureus (ATCC 25623), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231) and clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA). Antimicrobial activity of G-AgNPs was compared with the chemically synthesized silver nanoparticles (AgNPs). The formation of G-AgNPs is a promising novel approach to enhance the biologically activeness of silver nanoparticle and to increase the water solubility of garcinol which provides a significant array of therapeutic applications.