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Traumatic resin defense in Norway spruce (Picea abies): methyl jasmonate-induced terpene synthase gene expression, and cDNA cloning and functional characterization of (+)-3-carene synthase.

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Picea abies (L.) Karst. (Norway spruce) employs constitutive and induced resin terpenoids as major chemical and physical defense-shields against insects and pathogens. In recent work, we showed that a suite of terpenoids, monoterpenoids and diterpenoids was induced in stems of Norway spruce after

Biotransformation of (1S)-2-carene and (1S)-3-carene by Picea abies suspension culture.

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Biotransformation of (1S)-2-carene and (1S)-3-carene by Picea abies suspension culture led to the formation of oxygenated products. (1S)-2-Carene was transformed slowly and the final product was identified as (1S)-2-caren-4-one. On the other hand, the transformation of (1S)-3-carene was rapid and

Composition and antibacterial activity of Abies balsamea essential oil.

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The antibacterial activity of the essential oil of Abies balsamea (balsam fir) was evaluated against Escherichia coli and Staphylococcus aureus. The essential oil of A. balsamea was found to be inactive against E. coli (>100 microg/mL) and active against S. aureus, with an MIC of 56 microg/mL. The

Monoterpene synthases of Pinus contorta and related conifers. A new class of terpenoid cyclase.

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A cell-free extract from the xylem of lodgepole pine (Pinus contorta) catalyzes the conversion of [1-3H1]geranyl pyrophosphate to a variety of monoterpene olefins found in lodgepole pine oleoresin. This monoterpene synthase activity is similar to previously described terpenoid cyclases from grand

Spruce budworm growth, development and food utilization on young and old balsam fir trees.

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Laboratory rearing of spruce budworm, Choristoneura fumiferana, in conjunction with field rearing, gravimetric analyses, a transfer experiment, and foliage chemical analyses at six dates during the period of budworm feeding activity indicated that the age of balsam fir, Abies balsamea, trees

Radical Scavenging Activity of the Essential Oil of Silver Fir (Abies alba).

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The essential oil of silver fir (Abies alba) is known to help respiratory system and have easing and soothing effect for muscle. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities of silver fir (Abies alba) essential oil. The composition of the

Targeted proteomics using selected reaction monitoring reveals the induction of specific terpene synthases in a multi-level study of methyl jasmonate-treated Norway spruce (Picea abies).

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Induction of terpene synthase (TPS) gene expression and enzyme activity is known to occur in response to various chemical and biological stimuli in several species of spruce (genus Picea). However, high sequence identity between TPS family members has made it difficult to determine the induction

Age and space distributions of monoterpenes in fresh needles of Picea abies (L) Karst. Determined by gas chromatography-mass spectrometry.

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Monoterpenes (alpha- and beta-pinene, delta-3-carene, camphene, alpha-phellandrene and limonene) were determined by Gas Chromatography-Mass Spectrometry in fresh needles of Picea abies (L) Karst. situated in three ecologically different regions of Moravia. Through the use of cryogenic grinding for

The influence of Ceratocystis polonica inoculation and methyl jasmonate application on terpene chemistry of Norway spruce, Picea abies.

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Constitutive and inducible terpene production is involved in conifer resistance against bark beetles and their associated fungi. In this study 72 Norway spruce (Picea abies) were randomly assigned to methyl jasmonate (MJ) application, inoculation with the bluestain fungus Ceratocystis polonica, or

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