5 rezultate
alpha-Trichosanthin, a eukaryotic ribosome-inactivating protein from Trichosanthes kirilowii, inhibits the replication of the human immunodeficiency virus (HIV) in vitro. The alpha-trichosanthin gene was placed under the transcriptional control of a tobamovirus subgenomic promoter in a plant RNA
Saline extracts of Trichosanthes kirilowii root tubers and Momordica charantia seeds were subjected to differential acetone fractionation. The fraction that precipitated after addition of 0.8 vol. acetone was designated API, the fraction that precipitated after addition of another 1.2 vol. acetone
The complete amino acid sequence of a new abortifacient protein, karasurin, was determined. Karasurin, which was isolated from fresh root tubers of Trichosanthes kirilowii Maximowicz var, japonicum Kitamura (Cucurbitaceae), was a highly basic protein with pI 10.1 and molecular weight of 28,000.
OBJECTIVE
To construct and express a trichosanthin(TCS)gene mutant and purify the expressed product.
METHODS
Predict the potential antigenic determinant on TCS molecule by computer modeling and induce site-directed mutation. Amplify gene mutant TCS(RL28-29CG); by PCR using the genomic DNA of
OBJECTIVE
To construct and express a trichosanthin (TCS) gene mutant and purify the expressed product in E.coli.
METHODS
The potential antigenic determinant was predicted on TCS molecule by computer modeling and induced for site-directed mutation. The gene mutant TCS(FYY163-165CSA); was amplified by