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Biosynthesis of the sesquiterpenic phytoalexin capsidiol in elicited root cultures of chili pepper (Capsicum annuum).

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The biosynthesis of the sesquiterpenic phytoalexin capsidiol was investigated using in vitro root cultures of chili pepper (Capsicum annuum) elicited with cellulase. Optimal concentrations of cellulase and sucrose for capsidiol production were established. A simple spectrophotometric procedure to

Cellulase elicitor induced accumulation of capsidiol in Capsicum annumm L. suspension cultures.

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When growth-phase cell suspension cultures of Capsicum annuum were treated with cellulase-elicitor preparation at 3 microg/ml, the level of capsidiol was transiently increased in the culture media rather than in the cells reaching its maximum approx 24 h after treatment. With methyl jasmonate it

Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages

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Capsicum annuum is one of the most important horticultural crops worldwide. Anthracnose disease (Colletotrichum spp.) is a major constraint for chili production, causing substantial losses. Capsidiol is a sesquiterpene phytoalexin present in pepper fruits that can enhance plant resistance. The

Cloning and bacterial expression of sesquiterpene cyclase, a key branch point enzyme for the synthesis of sesquiterpenoid phytoalexin capsidiol in UV-challenged leaves of Capsicum annuum.

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Sesquiterpene cyclase, a branch point enzyme in the general isoprenoid pathway for the synthesis of phytoalexin capsidiol, was induced in detached leaves of Capsicum annuum (pepper) by UV treatment. The inducibility of cyclase enzyme activities paralleled the absolute amount of cyclase protein(s) of

Flagellin of Bacillus amyloliquefaciens works as a resistance inducer against groundnut bud necrosis virus in chilli (Capsicum annuum L.).

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Groundnut bud necrosis virus (GBNV), a member of the genus Tospovirus, has an extensive host range and is associated with necrosis disease of chilli (Capsicum annuum L.), which is a major threat to commercial production. Plant growth promoting rhizobacteria (PGPR) have been investigated for their

Molecular characterization of pathogen-inducible bi-directional promoter from hot pepper (Capsicum annuum)

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In hot pepper, the sesquiterpene phytoalexin capsidiol is catalyzed by the two final step enzymes, a sesquiterpene cyclase (EAS) and a hydroxylase (EAH) that are genetically linked and present as head-to-head orientation in the genome. Transcriptomic analysis revealed that a subset of EAS and EAH is

Biochemical and molecular tools for the production of useful terpene products from pepper (Capsicum annuum).

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Among other natural products such as colorants and flavorants, natural fungicides like the pepper phytoalexin capsidiol, and the related biochemical pathways, may be used for practical approaches. Key enzymes such as 3-hydroxy-3-methylglutaryl Coenzyme A: reductase, the farnesyl pyrophosphate

New constituents of sweet Capsicum annuum L. fruits and evaluation of their biological activity.

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Four new acyclic diterpene glycosides named capsianosides (1-4), together with 12 known compounds, were isolated from the fresh sweet pepper fruits of Capsicum annuum L., a plant used as a vegetable food, spice, and external medicine. The chemical structures of new natural compounds, as well as

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