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Polysomes from expanded tobacco leaves.

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The isolation of intact polysomes from leaves of tobacco (Nicotiana tabacum L.) is dependent on the age and state of development of leaves. Undegraded polysomes from young leaves in the early stages of expansion can be isolated easily by extracting the leaves in ice-cold extraction buffer (200 mM

Heat-shock proteins are induced in unstressed leaves of Nicotiana attenuata (Solanaceae) when distant leaves are stressed.

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In Nicotiana attenuata, systemic induction of heat-shock proteins (Hsps) was detected in response to the treatment of single leaves by either heat shock, mechanical damage, or exogenous application of methyl jasmonate (MJ). All treatments increased the abundance of members of the 70-kD Hsp (Hsp70)

Antibacterial activity of Nicotiana tabacum leaves.

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Nicotiana tabacum leaf 60% methanolic extract exhibited antibacterial activity against six out of nine bacterial isolates at a concentration of 25 mg/ml.

Antiviral sesquiterpenes from leaves of Nicotiana tabacum.

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Three unreported sesquiterpenes possessing two new skeletons, tabasesquiterpenes A-C (1-3), together with three known sesquiterpenes (3-6) were isolated from the leaves of Nicotiana tabacum. Their structures were determined mainly by spectroscopic methods, including extensive 1D- and 2D-NMR

Transient Transactivation Studies in Nicotiana benthamiana Leaves.

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Transitory gene expression systems in Nicotiana benthamiana leaves, in combination with the use of gene silencing suppressors as the p19 or HC-pro proteins that allow for elevated levels of gene expression, have proven to be a highly versatile tool to analyze transcriptional function of DNA binding

Genotoxicity of Nicotiana tabacum leaves on Helix aspersa.

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Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus

Duvatrienediols in cuticular wax of Burley tobacco leaves.

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4, 8, 13-Duvatriene-1, 3-diol diastereoisomers have been identified in the cuticular wax of fresh Burley tobacco leaves. Their structures were determined by gas-liquid chromatography, mass spectrometry, and infrared and ultraviolet spectroscopy. Butylboronic acid derivatives of the alpha,

Long-chain aldehyde-forming activity in tobacco leaves.

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Long-chain aldehydes (LCA), such as pentadecanal (PD), (8Z,11Z)-heptadecadienal (HDD) and (8Z,11Z,14Z)-heptadecatrienal (HDT), were identified in the essential oils obtained from fresh green tobacco leaves (Nicotiana tabacum cv. BY2 and N. tabacum cv. MC). PD, HDD and HDT were found to be produced

Noreudesmane sesquiterpenoids from the leaves of Nicotiana tabacum.

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Six new 14-noreudesmane sesquiterpenoids, nicotabacosides A-F (1-6), along with five known sesquiterpenoids (7-11), were isolated from the leaves of Nicotiana tabacum. The structures of compounds 1-6 were elucidated as isorishitin 3-O-β-D-glucopyranoside (1), rishitin 3-O-β-D-glucopyranoside (2),

Ethylene evolution from tobacco leaves irradiated with UV-B.

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Seedlings of Nicotiana tabacum L. (cv. Petit Havana SR1) were grown in the presence or absence of ultraviolet-B (UV-B, 290-320 nm) irradiation. The evolution of ethylene from the leaves, the content of 1-aminocyclopropane-1-carboxylic acid (ACC), an endogenous precursor of ethylene, and the activity

Genetic variation in alkaloid accumulation in leaves of Nicotiana.

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Alkaloids are plant secondary metabolites that are widely distributed in Nicotiana species and contribute greatly to the quality of tobacco leaves. Some alkaloids, such as nornicotine and myosmine, have adverse effects on human health. To reduce the content of harmful alkaloids in tobacco leaves

Efflux of sucrose from minor veins of tobacco leaves.

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Mature leaves import limited amounts of nutrient when darkened for prolonged periods. We tested the hypothesis that import is restricted by the apoplast-phloem loading mechanism, ie., as sucrose exits the phloem of minor veins it is retrieved by the same tissue, thus depriving the mesophyll of

Aluminum uptake and disease resistance in Nicotiana rustica leaves.

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The comparative effectiveness of aluminum hydroxide and aluminum chloride has been studied in the development of bacterial wilt infection on leaves of Nicotiana rustica cv. Gansu yellow flower. We have analyzed the changes of foliar H(2)O(2) content, as well as of non-enzymatic and enzymatic

Mitochondrial preparations from the leaves of tobacco (Nicotiana tabacum).

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Developmental Instability in Leaves and Flowers of NICOTIANA TABACUM.

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