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7 rezultate
Purification and characterization of p-coumaroyl-D-glucose hydroxylase of sweet potato (Ipomoea batatas) roots.
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p-Coumaroyl-D-glucose hydroxylase in sweet potato (Ipomoea batatas Lam.) has been purified to apparent electrophoretic homogeneity using a combination of anion-and cation-exchange, hydrophobic and gel filtration chromatography. The purified enzyme was a monomer with a molecular weight of 33,000 and
Effects of Raw Potato Starch with High Resistant Starch Levels on Cecal Fermentation Properties in Rats.
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The effects of potato starch, isolated from Snowden (SD) and Kitahime (KH) varieties, on cecal fermatation properties in rats were evaluated. In high-amylose cornstarch (HAS), SD and KH groups, cecal acetate and total short-chain fatty acid concentrations were increased and cecal pH was lowered
Effects of potato fiber and potato-resistant starch on biomarkers of colonic health in rats fed diets containing red meat.
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The effects of red meat consumption with and without fermentable carbohydrates on indices of large bowel health in rats were examined. Sprague-Dawley rats were fed cellulose, potato fiber, or potato-resistant starch diets containing 12% casein for 2 wk, then similar diets containing 25% cooked beef
Development of Low-Cost Paper-Based Biosensor of Polyphenol Oxidase for Detection of Phenolic Contaminants in Water and Clinical Samples
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In the present work, polyphenol oxidase (PPO) enzyme was purified from potato peel using three-phase partitioning (TPP). In this method, ammonium sulfate and t-butanol were added to precipitate the protein/enzyme from the crude aqueous extract. The PPO enzyme precipitated as an interfacial layer
4-Coumaroyl coenzyme A 3-hydroxylase activity from cell cultures of Lithospermum erythrorhizon and its relationship to polyphenol oxidase.
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A 4-coumaroyl-CoA 3-hydroxylase activity was purified 4600-fold from cell cultures of Lithospermum erythrorhizon. The enzyme showed a molecular mass of 42,400 +/- 1700 Da in gel chromatography and required ascorbate, NADH, or NADPH as cofactors. 4-Coumaroyl-CoA, 4-coumarate, p-cresol, and several
Interactive effects of protein and carbohydrates on production of microbial metabolites in the large intestine of growing pigs.
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The study aimed at determining the effect of protein type and indigestible carbohydrates on the concentration of microbial metabolites in the large intestine of pigs. The experiment involved 36 pigs (15 kg initial body weight) divided into six groups, fed cereal-based diets with highly digestible
Volatile flavor components of stored nonfat dry milk.
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Nonfat dry milk (NDM) is widely used both as an ingredient in other preparations and for direct consumption. Flavor quality of NDM is a critical parameter because it can directly impact final product quality. Flavors can be formed in NDM during subsequent storage. Identification of compounds
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