Russian
Albanian
Arabic
Armenian
Azerbaijani
Belarusian
Bengali
Bosnian
Catalan
Czech
Danish
Deutsch
Dutch
English
Estonian
Finnish
Français
Greek
Haitian Creole
Hebrew
Hindi
Hungarian
Icelandic
Indonesian
Irish
Italian
Japanese
Korean
Latvian
Lithuanian
Macedonian
Mongolian
Norwegian
Persian
Polish
Portuguese
Romanian
Russian
Serbian
Slovak
Slovenian
Spanish
Swahili
Swedish
Turkish
Ukrainian
Vietnamese
Български
中文(简体)
中文(繁體)
Life Sciences 2019-May

Lycium barbarum polysaccharides promote osteoblasts viability by regulating microRNA-17/PTEN.

Только зарегистрированные пользователи могут переводить статьи
Войти Зарегистрироваться
Ссылка сохраняется в буфер обмена
Wenyong Liang
Zhen Yue

Ключевые слова

абстрактный

Lycium barbarum polysaccharides (LBPs) have been reported to promote the proliferation of osteoblasts. Further, the proliferation of osteoblasts has been proved to be associated with microRNA (miR)-17 which targets phosphatase and tensin homolog (PTEN) 3'-untranslated region (UTR) for regulating phosphoinositide 3 kinase/protein kinase B (PI3K/AKT) signaling pathway. Consequently, we aimed to explore a miR-17-relative mechanism by which LBPs mediates proliferation of osteoblasts.LBPs were used to stimulate MC3T3-E1 cells, and SF1670 was applied to repress PTEN. miR-17 inhibitor was transfected into cells for down-regulating miR-17. The cell viability and migration were examined by cell counting kit-8 (CCK-8) and 24-well Transwell migration assay, respectively. The expression of Cyclin D1, matrix metallopeptidase (MMP)-2 and MMP-9 was analyzed using Western blot. Quantitative reverse transcription PCR (qRT-PCR) was conducted to detect miR-17. The expression of PTEN and phosphorylation of PI3K and AKT were analyzed by Western blot.We demonstrated that LBPs promoted osteoblasts viability, facilitated migration and positively regulated the expression of miR-17. Additionally, miR-17 inhibitor abolished the positive effects of LBPs on the osteoblasts. Molecularly, miR-17 inhibited the expression of PTEN by targeting its 3'-untranslated region (3'-UTR). Mechanically, LBPs diminished PTEN expression and facilitated the phosphorylation of PI3K and AKT, while the decreased expression of PTEN and enhanced phosphorylation of PI3K and AKT were reversed by miR-17 inhibitor.LBPs might fortify osteoblasts viability by up-regulating miR-17 to target PTEN and consequently trigger PI3K/AKT pathway.

Присоединяйтесь к нашей
странице facebook

Самая полная база данных о лекарственных травах, подтвержденная наукой

  • Работает на 55 языках
  • Травяные лекарства, подтвержденные наукой
  • Распознавание трав по изображению
  • Интерактивная карта GPS - отметьте травы на месте (скоро)
  • Прочтите научные публикации, связанные с вашим поиском
  • Ищите лекарственные травы по их действию
  • Организуйте свои интересы и будьте в курсе новостей исследований, клинических испытаний и патентов

Введите симптом или заболевание и прочтите о травах, которые могут помочь, введите лекарство и узнайте о болезнях и симптомах, против которых оно применяется.
* Вся информация основана на опубликованных научных исследованиях.

Google Play badgeApp Store badge