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echinococcosis/protease

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Страница 1 от 27 полученные результаты

Alveolar hydatid cyst induced amyloid enhancing factor (AEF): physicochemical properties and abolition of AEF activity by serine protease inhibitors.

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Cell free supernatants prepared from amyloidotic liver, unfractionated and fractionated peritoneal and spleen cells from casein stimulated (16 h post-injection) or alveolar hydatid cyst-infected (7 or 12 weeks post-infection, p. i.) C57BL/6J mice were used to assess amyloid enhancing factor (AEF)

[Appearance of specific protective proteases in subjects with echinococcosis].

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Cloning and Characterization of Two Potent Kunitz Type Protease Inhibitors from Echinococcus granulosus.

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The tapeworm Echinococcus granulosus is responsible for cystic echinococcosis (CE), a cosmopolitan disease which imposes a significant burden on the health and economy of affected communities. Little is known about the molecular mechanisms whereby E. granulosus is able to survive in the hostile

Purification of a recombinant glutathione transferase from the causative agent of hydatidosis, Echinococcus granulosus.

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This practical class activity was designed to introduce students to recombinant protein expression and purification. The principal goal is to shed light on basic aspects concerning recombinant protein production, in particular protein expression, chromatography methods for protein purification, and

Characterization of excretory-secretory products from protoscoleces of Echinococcus granulosus and evaluation of their potential for immunodiagnosis of human cystic echinococcosis.

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This study describes, for the first time, the characterization of excretory-secretory antigens (ES-Ag) from Echinococcus granulosus protoscoleces, evaluating their usefulness in the immunodiagnosis of human cystic echinococcosis. ES-Ag were obtained from the first 50 h maintenance of protoscoleces

Alveolar echinococcosis of the liver in an adult with human immunodeficiency virus type-1 infection.

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We describe a patient with human immunodeficiency virus type-1 (HIV) infection and alveolar echinococcosis (AE) with a focus on two messages. Despite being severely immunocompromised over years the patient exhibited a long-term asymptomatic course of AE. This is in clear contrast to reports

Echinococcosis and allergy.

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The larval stages of Echinococcus granulosus and E. multilocularis are involved in parasitic diseases in humans: cystic echinococcosis (CE) ("hydatid disease") and alveolar echinococcosis (AE), respectively. Both diseases and parasites have tight links with allergy because of the immunological

Kunitz type protease inhibitor EgKI-1 from the canine tapeworm Echinococcus granulosus as a promising therapeutic against breast cancer.

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EgKI-1, a member of the Kunitz type protease inhibitor family, is highly expressed by the oncosphere of the canine tapeworm Echinococcus granulosus, the stage that is infectious to humans and ungulates, giving rise to a hydatid cyst localized to the liver and other organs. Larval protoscoleces,

Further characterization of monoclonal antibodies to Echinococcus granulosus antigen 5 and antigen B.

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Two monoclonal antibodies (24.14, 61A12) to Echinococcus granulosus Antigen 5 and two (31.15 and 39B3) to Antigen B were further characterized using modified sheep hydatid cyst fluid antigens (SHCF) in ELISA. None of these four monoclonals were directed against carbohydrate or lipid epitopes of SHCF

Biochemical nature and cellular origin of amyloid enhancing factor (AEF) as determined by anti-AEF antibody.

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Low ionic strength acidic buffer, Sephadex G-200 and Benzamidine-Sepharose (BZ) gel chromatography, have been used for the partial purification of alveolar hydatid cyst (AHC) induced amyloid enhancing factor (AEF). BZ-gel bound AEF (AEF-BZ) demonstrated AEF activity in the mouse bioassay,

[Expression, purification and identification of Echinococcus granulosus recombinant antigen B].

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OBJECTIVE To purify and identify recombinant antigen B of hydatid disease. METHODS The recombinant plasmid pMalc2x-AgB was expressed in E. coli JM109. The fusion protein rAgB-MBP was made up of antigen B and MBP (maltose binding protein) which was designed to absorb the antigen B onto the amylose

Isolation and characterization of a secretory component of Echinococcus multilocularis metacestodes potentially involved in modulating the host-parasite interface.

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Echinococcus multilocularis metacestodes are fluid-filled, vesicle-like organisms, which are characterized by continuous asexual proliferation via external budding of daughter vesicles, predominantly in the livers of infected individuals. Tumor-like growth eventually leads to the disease alveolar

Diagnosis of human fasciolosis in the Gilan province of Northern Iran: application of cathepsin L-ELISA.

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Fasciolosis, or liver fluke disease, caused by parasites of the genus Fasciola is emerging as an important disease in man, particularly in countries such as Bolivia, Peru and Egypt. Several outbreaks of this disease recently occurred in the Gilan province of Northern Iran and in 1999 alone over

Recent advances in characterization of Echinococcus antigen B.

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Antigen B (AgB) in hydatid cyst fluid of Echinococcus granulosus is a polymeric lipoprotein of 160 kDa and a highly immunogenic major antigen in echinococcal infection. The antigen is comprised of a group of subunit monomers of approximately 8 kDa in molecular size. Recent studies have revealed that

A family of diverse Kunitz inhibitors from Echinococcus granulosus potentially involved in host-parasite cross-talk.

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The cestode Echinococcus granulosus, the agent of hydatidosis/echinococcosis, is remarkably well adapted to its definitive host. However, the molecular mechanisms underlying the successful establishment of larval worms (protoscoleces) in the dog duodenum are unknown. With the aim of identifying
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