avena/phosphatase

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Effect of vanadate, molybdate, and azide on membrane-associated ATPase and soluble phosphatase activities of corn roots.

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The effects of vanadate, molybdate, and azide on ATP phosphohydrolase (ATPase) and acid phosphatase activities of plasma membrane, mitochondrial, and soluble supernatant fractions from corn (Zea mays L. WF9 x MO17) roots were investigated. Azide (0.1-10 millimolar) was a selective inhibitor of pH

Evidence of active NADP(+) phosphatase in dormant seeds of Avena sativa L.

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Freshly-harvested seeds of Avena sativa L. do not germinate when imbibed at temperatures higher than 25 degrees C. This high temperature dormancy is due to the seed coats, and to the low activities of glycolysis and the oxidative pentose phosphate pathway (OPP) in the embryo. The analysis by

Cytochemical localization of ATPase activity in oat roots localizes a plasma membrane-associated soluble phosphatase, not the proton pump.

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Cytochemical techniques employing lead-precipitation of enzymically released inorganic phosphate have been widely used in attempts to localize the plasma membrane proton pump (H(+)-ATPase) in electron micrographs. Using Avena sativa root tissue we have performed a side-by-side comparison of ATPase

In situ localization of faba bean and oat legumin-type proteins in transgenic tobacco seeds by a highly sensitive immunological tissue print technique.

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We have used a highly sensitive immunological tissue print technique to study cell- and tissue-specific expression of heterologous genes in transgenic plants. Primary polyclonal antibodies, raised against legumin of faba bean (Vicia faba L.) and 12S globulin of oat (Avena sativa L.) were used to

The effect of Korean red ginseng on mesenchymal stem cells from healthy and osteoporotic human bone marrow.

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Osteoporosis is a disease characterized by an increase in bone fragility as a result of decreased bone mass and weakening of the bone structure. There are studies on the relationship between osteoporosis and hearing and balance system. The goal of this study was to compare the

Phosphate-limited oat. The plasma membrane and the tonoplast as major targets for phospholipid-to-glycolipid replacement and stimulation of phospholipases in the plasma membrane.

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We recently reported that cultivation of oat (Avena sativa L.) without phosphate resulted in plasma membrane phosphoglycerolipids being replaced to a large extent by digalactosyldiacylglycerol (DGDG) (Andersson, M. X., Stridh, M. H., Larsson, K. E., Liljenberg, C., and Sandelius, A. S. (2003) FEBS

Two new secondary metabolites isolated from Avena sativa L. (Oat) seedlings and their effects on osteoblast differentiation

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Seedlings of natural crops are valuable sources of pharmacologically active phytochemicals. In this study, we aimed to identify new active secondary metabolites in Avena sativa L. (oat) seedlings. Two new compounds, avenafuranol (1) and diosgenoside (2), along with eight known compounds (3-10) were

Mechanisms of oat (Avena sativa L.) acclimation to phosphate deficiency.

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UNASSIGNED Deficiency of available forms of phosphorus is common in most soils and causes reduction of crop plants growth and yield. Recently, model plants responses to phosphate (Pi) deficiency have been intensively studied. However, acclimation mechanisms of cereals like oat (Avena sativa L.), to

Responses of enzymically isolated aleurone cells of oat to gibberellin a(3).

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Oat (Avena sativa L.) aleurone layer cells (spheroplasts) were isolated by maceration of the aleurone layer with a mixture of commercially available cellulase and pectinase. About 20% of the cells present in intact layers were released as spheroplasts and 79 +/- 9% of the spheroplast population was

Changes in starch content in oat (Avena sativa) shoot pulvini during the gravitropic response.

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In order to determine if components of the signal transduction pathway are involved in starch metabolism during the gravitropic response, the effects of inhibitors of phosphoprotein phosphatases and protein kinases (OA), and calcium channel blockers (LaCl3), on gravitropic bending and starch levels

Covalent binding sites of victorin in oat leaf tissues detected by anti-victorin polyclonal antibodies.

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Polyclonal antibodies against victorin, the host-specific toxin produced by Cochliobolus victoriae, were raised in rabbits immunized with a victorin-bovine serum albumin conjugate. The antibodies were purified from serum by protein A column chromatography and characterized by indirect and direct

Immunocytolocalization of Plasma Membrane H-ATPase.

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The localization of plasma membrane H(+)-ATPase has been studied at the optical microscope level utilizing frozen and paraffin sections of Avena sativa and Pisum sativum, specific anti-ATPase polyclonal antibody, and second antibody coupled to alkaline phosphatase. In leaves and stems the ATPase is

Cover crops influence soil microorganisms and phytoextraction of copper from a moderately contaminated vineyard.

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We investigated the ability of summer (Avena sativa [oat], Trifolium incarnatum [crimson clover], Chenopodium [goosefoot]) and winter (Vicia villosa [hairy vetch], Secale Cereale L. [Rye], Brassica napus L. partim [rape]) cover crops, including a mixed species treatment, to extract copper from an

Inhibition of IAA-induced elongation in Avena coleoptile segments by lead: a physiological and an electron microscopic study.

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A high resolution growth measuring apparatus was used to demonstrate the inhibition of auxin-induced cell elongation in oat coleoptile segments (Avena sativa L. var Holden) by lead at concentrations ranging from 2 x 10-6 M to 2 x 10-3 M. The inhibition was immediate, having no measurable lag period.

Regulation of H Excretion : ROLE OF PROTEIN RELEASED BY OSMOTIC SHOCK.

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When the protoplasts of peeled oat leaf segments (Avena sativa L.) expand after a brief plasmolysis (osmotic shock), fusicoccin-enhanced H(+) excretion is reduced and protein is released to the rehydration medium. This shock protein seems to arise from the cell surface, not from the interior of

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