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Antitumor effects of curcin from seeds of Jatropha curcas.

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OBJECTIVE To study the antitumor effects of curcin from Jatropha curcas. METHODS Antitumor activity of curcin was tested by MTT assay. The N-glycosidase activity of curcin was determined by characterization of R-fragment in gel. A cell-free system, rabbit reticulocyte lysate, was introduced to

Expression, purification and anti-tumor activity of curcin.

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Curcin, purified from the seeds of Jatropha curcas, can be used as a cell-killing agent. Understanding the anti-tumor activity of the recombinant protein of curcin is important for its application in clinical medicine. The segment encoding the mature protein of curcin was inserted into Escherichia

Lathyrane diterpenoids from Jatropha podagrica and their antitumor activities in human osteosarcoma cells

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Two new lathyrane-type diterpenoids, jatropodagins A and B (1 and 2), and five known analogues (3-7), were isolated from the stems of Jatropha podagrica. Their structures and absolute configurations were elucidated by spectroscopic data and calculated ECD analyses.

Study of the anticancer potential of Yemeni plants used in folk medicine.

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The present work evaluated the anticancer activity of methanol extracts from 24 plants used in Yemeni traditional medicine. To evaluate the in vitro cytotoxic potency of the investigated extracts, an established microtiter plate assay based on cellular staining with crystal violet was used with 5

[Purification and anti-cancer activity of ricin].

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OBJECTIVE To extract and purify ricin from castor beans and to evaluate its anti-cancer activity. METHODS Ricin was purified from castor beans according the modified method of Nicolson and Blaustin. The lectins were extracted in 0.01 mol/L phosphate buffered saline and isolated in the 40% to 80%

Antitumor agents from Jatropha macrorhiza (Euphorbiaceae) III: acetylaleuritolic acid.

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The triterpene acetylaleuritolic acid was isolated from Jatropha macrorhiza (Euphorbiaceae) and showed tumor-inhibitory properties toward the P-388 lymphocytic leukemia test system. The compound was identified by means of IR, PMR, and mass spectrometry and by its transformation into the known methyl

Studies on the antitumor lectins isolated from the seeds of Ricinus communis (castor bean).

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Three toxic proteins and one agglutinin were purified from the seeds of Ricinus communis by a simple and fast method using Sepharose 4-B affinity chromatography followed by Sephadex G-100 gel filtration. The weakly adsorbed ricins A and B were retarded and eluted with the buffer from the affinity

Antitumor Activity of Diterpenoids from Jatropha gossypiifolia: Cell Cycle Arrest and Apoptosis-Inducing Activity in RKO Colon Cancer Cells.

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Nine new minor diterpenoids, jatrogossones A-I (1-9), and six known analogues (10-15) were separated from an extract of the branches and leaves of Jatropha gosspiifolia. Compounds 4-6 and 10, possessing a 5/11 fused-ring skeleton, and 8, 9, and 13, with a 5/9/5 fused-ring skeleton, represent rare

Anticancer activity of five forest crops used in African folklore: antiproliferative and pro-apoptotic effects.

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Acalypha wilkesiana, Caesalpinia bonduc, Jatropha multifida, Momordica charantia and Picralima nitida used in African folklore for treating cancer were investigated. All extracts except J. multifida resulted in no significant alteration in cell cycle distribution and apoptosis in MCF-7 and BT-20.

A novel anticancer diterpenoid from Jatropha gossypifolia.

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Jatropha gossypifolia root bark is used in ethnomedicine for bacterial infections and cancer of the lungs. Phytochemical investigation resulted in the isolation and characterization of a potent anticancer and novel lathyrane diterpenoid compound, abiodone, with an unusual methylene group. The

Antitumor agents from Jatropha macrorhiza (Euphorbiaceae). II. Isolation and characterization of jatrophatrione.

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Antitumor agents from Jatropha macrorhiza (Euphorbiaceae) I: isolation and characterization of jatropham.

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Irritant and antineoplastic principles of some species of the genus jatropha (euphorbiaceae).

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Alkannins and shikonins: a new class of wound healing agents.

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Alkannins and Shikonins (A/S) are chiral-pairs of naturally occurring isohexenylnaphthazarins. They are found in the external layer of the roots of at least a hundred and fifty species that belong mainly to the genera Alkanna, Lithospermum, Echium, Onosma and Arnebia of the Boraginaceae family.

The effect of curcin from Jatropha curcas on apoptosis of mouse sarcoma-180 cells.

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Curcin is a ribosome-inactivating protein expressed in the endosperm of Jatropha curcas. Curcin can inhibit growth of mouse sarcoma-180 cells. At a curcin concentration of 100 μg/ml, mouse sarcoma-180 cell growth was inhibited by over 40% after seven days of incubation. Acridine orange staining and

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